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Statistics

Number of enzymes: 1
Total Number of designed primers: 12
Number of PGTM primers:     2
Number of PMTM primers:     10

Enzyme Id:  K21995
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340815     NCBI Gene Symbol: LOC103340815
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 77A3      Other designations:   cytochrome P450 77A3
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 14592812 ...... 14595218
CDS Sequence 103340815
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GCAGT)2
Repeat start & end within CDS Repeat start: 444     Repeat end: 453
Forward primer Primer sequence:   CCAGCTCCGATCTCATCCAC     Tm(°C): 59.967     GC (%): 60     Size: 20
Reverse primer Primer sequence:   ATGGTAAGCCTGCAGTTGCT     Tm(°C): 59.961     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 253     End: 562     Product size (bp): 310
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Enzyme Id:  K21995
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340815     NCBI Gene Symbol: LOC103340815
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 77A3      Other designations:   cytochrome P450 77A3
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 14592812 ...... 14595218
CDS Sequence 103340815
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (ATG)3
Repeat start & end within CDS Repeat start: 649     Repeat end: 657
Forward primer Primer sequence:   GGGGCAAAAATCTCCGAGGA     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AACCGTTGAGCTCTTCCAGG     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 591     End: 892     Product size (bp): 302
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Enzyme Id:  K21995
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340815     NCBI Gene Symbol: LOC103340815
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 77A3      Other designations:   cytochrome P450 77A3
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 14592812 ...... 14595218
CDS Sequence 103340815
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAG)3
Repeat start & end within CDS Repeat start: 735     Repeat end: 743
Forward primer Primer sequence:   GGGGCAAAAATCTCCGAGGA     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AACCGTTGAGCTCTTCCAGG     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 591     End: 892     Product size (bp): 302
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Enzyme Id:  K21995
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340815     NCBI Gene Symbol: LOC103340815
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 77A3      Other designations:   cytochrome P450 77A3
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 14592812 ...... 14595218
CDS Sequence 103340815
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (AGG)3
Repeat start & end within CDS Repeat start: 1049     Repeat end: 1057
Forward primer Primer sequence:   GAGTGGGCTCTGCTCCATTT     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CTCAGTGACCCATGCAGTGT     Tm(°C): 59.964     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 966     End: 1235     Product size (bp): 270
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Enzyme Id:  K21995
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340815     NCBI Gene Symbol: LOC103340815
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 77A3      Other designations:   cytochrome P450 77A3
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 14592812 ...... 14595218
CDS Sequence 103340815
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GTGTTTGGGGCTCGTGTTTC     Tm(°C): 59.97     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGATGAGATCGGAGCTGGTG     Tm(°C): 59.967     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 29     End: 270     Product size (bp): 242
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Enzyme Id:  K21995
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331162     NCBI Gene Symbol: LOC103331162
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 77A3-like      Other designations:   cytochrome P450 77A3-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 8659234 ...... 8661359
CDS Sequence 103331162
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (TC)4
Repeat start & end within CDS Repeat start: 28     Repeat end: 35
Forward primer Primer sequence:   ACCCTCTCTTTCTCCTCTGCT     Tm(°C): 59.921     GC (%): 52.381     Size: 21
Reverse primer Primer sequence:   AGGTTTCCAACAACAGGCCA     Tm(°C): 60.033     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 6     End: 169     Product size (bp): 164
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Enzyme Id:  K21995
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331162     NCBI Gene Symbol: LOC103331162
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 77A3-like      Other designations:   cytochrome P450 77A3-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 8659234 ...... 8661359
CDS Sequence 103331162
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (AAATCC)2
Repeat start & end within CDS Repeat start: 106     Repeat end: 117
Forward primer Primer sequence:   CGGGCTGATCTTCTTGCTCA     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGGTTTCCAACAACAGGCCA     Tm(°C): 60.033     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 80     End: 169     Product size (bp): 90
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Enzyme Id:  K21995
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331162     NCBI Gene Symbol: LOC103331162
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 77A3-like      Other designations:   cytochrome P450 77A3-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 8659234 ...... 8661359
CDS Sequence 103331162
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (ACCCG)2
Repeat start & end within CDS Repeat start: 353     Repeat end: 362
Forward primer Primer sequence:   GTCCACGAAGCCTTGATCGA     Tm(°C): 60.109     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AAGCGTGCGGTTTTCAGAAC     Tm(°C): 59.971     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 297     End: 580     Product size (bp): 284
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Enzyme Id:  K21995
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331162     NCBI Gene Symbol: LOC103331162
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 77A3-like      Other designations:   cytochrome P450 77A3-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 8659234 ...... 8661359
CDS Sequence 103331162
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (AGGCCA)2
Repeat start & end within CDS Repeat start: 533     Repeat end: 544
Forward primer Primer sequence:   TCGAGAAAACGCCATGGACA     Tm(°C): 59.966     GC (%): 50     Size: 20
Reverse primer Primer sequence:   AAGCGTGCGGTTTTCAGAAC     Tm(°C): 59.971     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 488     End: 580     Product size (bp): 93
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Enzyme Id:  K21995
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331162     NCBI Gene Symbol: LOC103331162
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 77A3-like      Other designations:   cytochrome P450 77A3-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 8659234 ...... 8661359
CDS Sequence 103331162
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TCCGA)2
Repeat start & end within CDS Repeat start: 1261     Repeat end: 1270
Forward primer Primer sequence:   ATGCAGTTACTGAGCCCACC     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CATATCCTTCTCCCCACGCC     Tm(°C): 59.965     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 1159     End: 1372     Product size (bp): 214
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Enzyme Id:  K21995
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331162     NCBI Gene Symbol: LOC103331162
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 77A3-like      Other designations:   cytochrome P450 77A3-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 8659234 ...... 8661359
CDS Sequence 103331162
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GAGTG)2
Repeat start & end within CDS Repeat start: 1438     Repeat end: 1447
Forward primer Primer sequence:   ATGCAGTTACTGAGCCCACC     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACTCCAACTTCCCGGCAAAA     Tm(°C): 60.106     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1159     End: 1491     Product size (bp): 333
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Enzyme Id:  K21995
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331162     NCBI Gene Symbol: LOC103331162
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 77A3-like      Other designations:   cytochrome P450 77A3-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 8659234 ...... 8661359
CDS Sequence 103331162
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GTTCTGAAAACCGCACGCTT     Tm(°C): 59.971     GC (%): 50     Size: 20
Reverse primer Primer sequence:   AATCGTCGATTCTCGGGTCG     Tm(°C): 59.971     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 561     End: 702     Product size (bp): 142
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