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Statistics

Number of enzymes: 1
Total Number of designed primers: 11
Number of PGTM primers:     2
Number of PMTM primers:     9

Enzyme Id:  K20769
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103334520     NCBI Gene Symbol: LOC103334520
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 94A1-like      Other designations:   cytochrome P450 94A1-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 4173854 ...... 4175796
CDS Sequence 103334520
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TTC)3
Repeat start & end within CDS Repeat start: 52     Repeat end: 60
Forward primer Primer sequence:   CCCTGGTCCTCCTTCCCTTA     Tm(°C): 59.956     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TTGGGGAGCTTGGTGTTGTT     Tm(°C): 60.033     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 28     End: 118     Product size (bp): 91
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Enzyme Id:  K20769
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103334520     NCBI Gene Symbol: LOC103334520
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 94A1-like      Other designations:   cytochrome P450 94A1-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 4173854 ...... 4175796
CDS Sequence 103334520
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CAATT)2
Repeat start & end within CDS Repeat start: 516     Repeat end: 525
Forward primer Primer sequence:   AAAAAGGCAACACCATCCGC     Tm(°C): 59.967     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CAGGTAAGCCGGATCGTACC     Tm(°C): 59.968     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 313     End: 605     Product size (bp): 293
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Enzyme Id:  K20769
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103334520     NCBI Gene Symbol: LOC103334520
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 94A1-like      Other designations:   cytochrome P450 94A1-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 4173854 ...... 4175796
CDS Sequence 103334520
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TCTCC)2
Repeat start & end within CDS Repeat start: 837     Repeat end: 846
Forward primer Primer sequence:   AGGCACTAGAGTCCGTGGAT     Tm(°C): 60.033     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGAACCATGTGAGAGCTGCC     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 817     End: 951     Product size (bp): 135
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Enzyme Id:  K20769
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103334520     NCBI Gene Symbol: LOC103334520
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 94A1-like      Other designations:   cytochrome P450 94A1-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 4173854 ...... 4175796
CDS Sequence 103334520
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GGACG)2
Repeat start & end within CDS Repeat start: 1155     Repeat end: 1164
Forward primer Primer sequence:   GGCAGCTCTCACATGGTTCT     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CTCTCTCTCCAGCCACCTCT     Tm(°C): 60.033     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 932     End: 1277     Product size (bp): 346
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Enzyme Id:  K20769
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103334520     NCBI Gene Symbol: LOC103334520
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 94A1-like      Other designations:   cytochrome P450 94A1-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 4173854 ...... 4175796
CDS Sequence 103334520
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (GA)4
Repeat start & end within CDS Repeat start: 1270     Repeat end: 1277
Forward primer Primer sequence:   GGGACGAAGGTGAAGAAGGG     Tm(°C): 60.036     GC (%): 60     Size: 20
Reverse primer Primer sequence:   CCGGGACCACCTTAAACCTC     Tm(°C): 60.036     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 1158     End: 1434     Product size (bp): 277
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Enzyme Id:  K20769
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103334520     NCBI Gene Symbol: LOC103334520
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 94A1-like      Other designations:   cytochrome P450 94A1-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 4173854 ...... 4175796
CDS Sequence 103334520
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AGCCC)2
Repeat start & end within CDS Repeat start: 1452     Repeat end: 1461
Forward primer Primer sequence:   AGAGGTGGCTGGAGAGAGAG     Tm(°C): 60.033     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TTTTTCCGGTGAGGTAGGCC     Tm(°C): 59.963     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1258     End: 1488     Product size (bp): 231
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Enzyme Id:  K20769
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103334520     NCBI Gene Symbol: LOC103334520
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 94A1-like      Other designations:   cytochrome P450 94A1-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 4173854 ...... 4175796
CDS Sequence 103334520
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CAGCAACAACACCAAGCTCC     Tm(°C): 59.969     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGATGGAGAGCTTCGGAGGA     Tm(°C): 60.033     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 95     End: 212     Product size (bp): 118
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Enzyme Id:  K20769
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103334522     NCBI Gene Symbol: LOC103334522
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 94A1-like      Other designations:   cytochrome P450 94A1-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 4192351 ...... 4194209
CDS Sequence 103334522
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CAATT)2
Repeat start & end within CDS Repeat start: 516     Repeat end: 525
Forward primer Primer sequence:   CCAAGTCTCCAGCCACGAAT     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAGGTAAGCCGGGTCGTATC     Tm(°C): 59.968     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 401     End: 605     Product size (bp): 205
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Enzyme Id:  K20769
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103334522     NCBI Gene Symbol: LOC103334522
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 94A1-like      Other designations:   cytochrome P450 94A1-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 4192351 ...... 4194209
CDS Sequence 103334522
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TCTCC)2
Repeat start & end within CDS Repeat start: 837     Repeat end: 846
Forward primer Primer sequence:   ACGAAGGCTCTAGAGTCCGT     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGAACCATGTGAGAGCTGCC     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 813     End: 951     Product size (bp): 139
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Enzyme Id:  K20769
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103334522     NCBI Gene Symbol: LOC103334522
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 94A1-like      Other designations:   cytochrome P450 94A1-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 4192351 ...... 4194209
CDS Sequence 103334522
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (GA)4
Repeat start & end within CDS Repeat start: 1270     Repeat end: 1277
Forward primer Primer sequence:   GGGACGAAGGTGAAGAAGGG     Tm(°C): 60.036     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TGCAGAAACGCCATCTCCTT     Tm(°C): 59.963     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1158     End: 1384     Product size (bp): 227
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Enzyme Id:  K20769
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103334522     NCBI Gene Symbol: LOC103334522
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 94A1-like      Other designations:   cytochrome P450 94A1-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 4192351 ...... 4194209
CDS Sequence 103334522
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CTTAACCTTGGCCTCCCTGG     Tm(°C): 60.034     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GAGCTTGGTGTTGTTGCTGG     Tm(°C): 59.969     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 14     End: 113     Product size (bp): 100
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