image


Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     1
Number of PMTM primers:     2

Enzyme Id:  K20495
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321679     NCBI Gene Symbol: LOC103321679
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 704B1      Other designations:   cytochrome P450 704B1
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 17017790 ...... 17019976
CDS Sequence 103321679
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CGT)3
Repeat start & end within CDS Repeat start: 234     Repeat end: 242
Forward primer Primer sequence:   GGATGCACGATTGGCTTGTC     Tm(°C): 59.901     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCTCCAAAGCTCTCCATCCG     Tm(°C): 60.179     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 190     End: 404     Product size (bp): 215
JBrowse View      JBrowse

Enzyme Id:  K20495
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321679     NCBI Gene Symbol: LOC103321679
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 704B1      Other designations:   cytochrome P450 704B1
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 17017790 ...... 17019976
CDS Sequence 103321679
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (TCCCAT)2gtagctgaaaagctatattcggagctcaaaaccttggaagaagatc
gagc(AAGAG)2
Repeat start & end within CDS Repeat start: 997     Repeat end: 1068
Forward primer Primer sequence:   GCAACTACTCTCTCCTGGGC     Tm(°C): 59.823     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GGACTGCAGGGTACAAACGA     Tm(°C): 59.966     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 960     End: 1221     Product size (bp): 262
JBrowse View      JBrowse

Enzyme Id:  K20495
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321679     NCBI Gene Symbol: LOC103321679
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 704B1      Other designations:   cytochrome P450 704B1
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 17017790 ...... 17019976
CDS Sequence 103321679
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TCGTTTGTACCCTGCAGTCC     Tm(°C): 59.966     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGTAAGCTTCAAGCCATGCG     Tm(°C): 59.901     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1202     End: 1589     Product size (bp): 388
JBrowse View      JBrowse