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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     3
Number of PMTM primers:     1
Number of Failed designed PMTM primers: 2

Enzyme Id:  K17991

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103318709     NCBI Gene Symbol: LOC103318709
Gene Aliases
Gene description & Other designations Description:   peroxygenase      Other designations:   peroxygenase
Chromosome, Strand & Exon count Chromosome:   LG1     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024126.1      Gene Start and end within genomic accession: 25825145 ...... 25826719
CDS Sequence 103318709
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TTTCAT)2
Repeat start & end within CDS Repeat start: 300     Repeat end: 311
Forward primer Primer sequence:   AGGGCTATGACTGCTCCTGA     Tm(°C): 60.032     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGCCCGGTGTATGTTTTCGA     Tm(°C): 59.965     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 123     End: 404     Product size (bp): 282
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Enzyme Id:  K17991

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103318709     NCBI Gene Symbol: LOC103318709
Gene Aliases
Gene description & Other designations Description:   peroxygenase      Other designations:   peroxygenase
Chromosome, Strand & Exon count Chromosome:   LG1     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024126.1      Gene Start and end within genomic accession: 25825145 ...... 25826719
CDS Sequence 103318709
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TCGAAAACATACACCGGGCA     Tm(°C): 59.965     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TCGAACAAGCTTCCGTCGAA     Tm(°C): 59.969     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 385     End: 679     Product size (bp): 295
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Enzyme Id:  K17991

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321432     NCBI Gene Symbol: LOC103321432
Gene Aliases
Gene description & Other designations Description:   probable peroxygenase 4      Other designations:   probable peroxygenase 4
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 16160203 ...... 16162770
CDS Sequence 103321432
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TTC)3
Repeat start & end within CDS Repeat start: 6     Repeat end: 14
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K17991

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321432     NCBI Gene Symbol: LOC103321432
Gene Aliases
Gene description & Other designations Description:   probable peroxygenase 4      Other designations:   probable peroxygenase 4
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 16160203 ...... 16162770
CDS Sequence 103321432
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GGAGAG)2
Repeat start & end within CDS Repeat start: 549     Repeat end: 560
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K17991

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321432     NCBI Gene Symbol: LOC103321432
Gene Aliases
Gene description & Other designations Description:   probable peroxygenase 4      Other designations:   probable peroxygenase 4
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 16160203 ...... 16162770
CDS Sequence 103321432
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TTTCGTGCAATTGGCTGTGG     Tm(°C): 59.968     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CATAGACACCGGAGTCGCTC     Tm(°C): 59.97     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 129     End: 294     Product size (bp): 166
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Enzyme Id:  K17991

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103323925     NCBI Gene Symbol: LOC103323925
Gene Aliases
Gene description & Other designations Description:   peroxygenase      Other designations:   peroxygenase
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 39105792 ...... 39108075
CDS Sequence 103323925
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CAATCTCATCGCTGCCGTTG     Tm(°C): 59.973     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCCATCAAAGCAGCGCCTAA     Tm(°C): 60.036     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 278     End: 662     Product size (bp): 385
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