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Statistics

Number of enzymes: 1
Total Number of designed primers: 24
Number of PGTM primers:     3
Number of PMTM primers:     21

Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103344052     NCBI Gene Symbol: LOC103344052
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103344052
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CA)4attccgaaccaattactcacttttcatgccaatctatgac(TA)5gaacc
atggacaaatcttcgaatgctctctacgaaacttcactca(AG)4
Repeat start & end within CDS Repeat start: 754     Repeat end: 864
Forward primer Primer sequence:   ACACCCTCGTACCACTCCTT     Tm(°C): 60.179     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGAGGCCAGGGAAGCAAATC     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 729     End: 953     Product size (bp): 225
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103344052     NCBI Gene Symbol: LOC103344052
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103344052
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TAGCT)2
Repeat start & end within CDS Repeat start: 1299     Repeat end: 1308
Forward primer Primer sequence:   AGCAGAGGAAAAGGGTGTCA     Tm(°C): 58.86     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CCTGGATACCCTTCTGGCAC     Tm(°C): 59.819     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 1172     End: 1422     Product size (bp): 251
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103344052     NCBI Gene Symbol: LOC103344052
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103344052
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (TTGCTT)2tgtgccagaagggtatccaggtag(TTACA)2
Repeat start & end within CDS Repeat start: 1391     Repeat end: 1436
Forward primer Primer sequence:   CACAAAGGTTGCTTACGCCG     Tm(°C): 60.385     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CATTGCTGGTGGGTAGTGGT     Tm(°C): 59.962     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1370     End: 1652     Product size (bp): 283
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103344052     NCBI Gene Symbol: LOC103344052
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103344052
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAA)3
Repeat start & end within CDS Repeat start: 1546     Repeat end: 1554
Forward primer Primer sequence:   GTGCCAGAAGGGTATCCAGG     Tm(°C): 59.819     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TGCACTATCCCTGCTATGCG     Tm(°C): 59.966     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1403     End: 1744     Product size (bp): 342
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103344052     NCBI Gene Symbol: LOC103344052
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103344052
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TCCACAGCACCTGGTTGTTT     Tm(°C): 60.034     GC (%): 50     Size: 20
Reverse primer Primer sequence:   AACTCCACAGGACCAGCATG     Tm(°C): 59.963     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 85     End: 418     Product size (bp): 334
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331413     NCBI Gene Symbol: LOC103331413
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11684545 ...... 11690773
CDS Sequence 103331413
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GAAAGA)2
Repeat start & end within CDS Repeat start: 120     Repeat end: 131
Forward primer Primer sequence:   CTGGCCATGGACTCCTCTTG     Tm(°C): 60.107     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TCCAAAGTGGCAGCTGTTGA     Tm(°C): 60.107     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 29     End: 366     Product size (bp): 338
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331413     NCBI Gene Symbol: LOC103331413
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11684545 ...... 11690773
CDS Sequence 103331413
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (CA)4
Repeat start & end within CDS Repeat start: 754     Repeat end: 761
Forward primer Primer sequence:   TGTACACCCCATCGTTCCAC     Tm(°C): 59.678     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGCATCACGGACCACAATGT     Tm(°C): 59.963     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 724     End: 1018     Product size (bp): 295
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331413     NCBI Gene Symbol: LOC103331413
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11684545 ...... 11690773
CDS Sequence 103331413
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TTG)3
Repeat start & end within CDS Repeat start: 1043     Repeat end: 1051
Forward primer Primer sequence:   ACATTGTGGTCCGTGATGCT     Tm(°C): 59.963     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CTGTTCAGCTCCTCACCCTG     Tm(°C): 60.037     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 999     End: 1237     Product size (bp): 239
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331413     NCBI Gene Symbol: LOC103331413
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11684545 ...... 11690773
CDS Sequence 103331413
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TAGCT)2
Repeat start & end within CDS Repeat start: 1299     Repeat end: 1308
Forward primer Primer sequence:   CAGGGTGAGGAGCTGAACAG     Tm(°C): 60.037     GC (%): 60     Size: 20
Reverse primer Primer sequence:   CCTGGATACCCTTCTGGCAC     Tm(°C): 59.819     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 1218     End: 1422     Product size (bp): 205
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331413     NCBI Gene Symbol: LOC103331413
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11684545 ...... 11690773
CDS Sequence 103331413
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAA)3
Repeat start & end within CDS Repeat start: 1546     Repeat end: 1554
Forward primer Primer sequence:   GTGCCAGAAGGGTATCCAGG     Tm(°C): 59.819     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TCACCCTTCTTGGCAACCAA     Tm(°C): 59.74     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1403     End: 1713     Product size (bp): 311
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331413     NCBI Gene Symbol: LOC103331413
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   plus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11684545 ...... 11690773
CDS Sequence 103331413
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GGCCCAGTGGAGTACCTCTA     Tm(°C): 60.032     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GCTATGTGCTCCGCAAATGG     Tm(°C): 59.97     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 405     End: 547     Product size (bp): 143
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331414     NCBI Gene Symbol: LOC103331414
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11734285 ...... 11742085
CDS Sequence 103331414
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (CA)4
Repeat start & end within CDS Repeat start: 754     Repeat end: 761
Forward primer Primer sequence:   ACACCCTCGTACCACTCCTT     Tm(°C): 60.179     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAAGGAAGCAAATCCGAGCG     Tm(°C): 59.902     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 729     End: 947     Product size (bp): 219
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331414     NCBI Gene Symbol: LOC103331414
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11734285 ...... 11742085
CDS Sequence 103331414
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (AG)4
Repeat start & end within CDS Repeat start: 857     Repeat end: 864
Forward primer Primer sequence:   ACACCCTCGTACCACTCCTT     Tm(°C): 60.179     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAAGGAAGCAAATCCGAGCG     Tm(°C): 59.902     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 729     End: 947     Product size (bp): 219
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331414     NCBI Gene Symbol: LOC103331414
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11734285 ...... 11742085
CDS Sequence 103331414
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TAGCT)2
Repeat start & end within CDS Repeat start: 1299     Repeat end: 1308
Forward primer Primer sequence:   AGCAGAGGAAAAGGGTGTCA     Tm(°C): 58.86     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CAAGCCACTTTCCATGCCAC     Tm(°C): 60.038     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1172     End: 1490     Product size (bp): 319
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331414     NCBI Gene Symbol: LOC103331414
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11734285 ...... 11742085
CDS Sequence 103331414
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TTGCTT)2
Repeat start & end within CDS Repeat start: 1391     Repeat end: 1402
Forward primer Primer sequence:   CACAAAGGTTGCTTACGCCG     Tm(°C): 60.385     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAAGCCACTTTCCATGCCAC     Tm(°C): 60.038     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1370     End: 1490     Product size (bp): 121
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331414     NCBI Gene Symbol: LOC103331414
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11734285 ...... 11742085
CDS Sequence 103331414
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAA)3
Repeat start & end within CDS Repeat start: 1546     Repeat end: 1554
Forward primer Primer sequence:   GTGGCATGGAAAGTGGCTTG     Tm(°C): 60.038     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGGGTGCGAATTGGGAGAAA     Tm(°C): 59.889     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1471     End: 1608     Product size (bp): 138
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331441     NCBI Gene Symbol: LOC103331441
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11852421 ...... 11856695
CDS Sequence 103331441
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CA)4attccgaaccaattactcacttttcatgccaatctatgac(TA)5gaacc
atggacaaatcttcgaatgctctctacgaaacttcactca(AG)4
Repeat start & end within CDS Repeat start: 754     Repeat end: 864
Forward primer Primer sequence:   ACACCCTCGTACCACTCCTT     Tm(°C): 60.179     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGAGGCCAGGGAAGCAAATC     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 729     End: 953     Product size (bp): 225
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331441     NCBI Gene Symbol: LOC103331441
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11852421 ...... 11856695
CDS Sequence 103331441
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TAGCT)2
Repeat start & end within CDS Repeat start: 1299     Repeat end: 1308
Forward primer Primer sequence:   AGCAGAGGAAAAGGGTGTCA     Tm(°C): 58.86     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CCTGGATACCCTTCTGGCAC     Tm(°C): 59.819     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 1172     End: 1422     Product size (bp): 251
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331441     NCBI Gene Symbol: LOC103331441
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11852421 ...... 11856695
CDS Sequence 103331441
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (TTGCTT)2tgtgccagaagggtatccaggtag(TTACA)2
Repeat start & end within CDS Repeat start: 1391     Repeat end: 1436
Forward primer Primer sequence:   CACAAAGGTTGCTTACGCCG     Tm(°C): 60.385     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CATTGCTGGTGGGTAGTGGT     Tm(°C): 59.962     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1370     End: 1652     Product size (bp): 283
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331441     NCBI Gene Symbol: LOC103331441
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11852421 ...... 11856695
CDS Sequence 103331441
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAA)3
Repeat start & end within CDS Repeat start: 1546     Repeat end: 1554
Forward primer Primer sequence:   GTGCCAGAAGGGTATCCAGG     Tm(°C): 59.819     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TGCACTATCCCTGCTATGCG     Tm(°C): 59.966     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1403     End: 1744     Product size (bp): 342
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331464     NCBI Gene Symbol: LOC103331464
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   9
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11843420 ...... 11846881
CDS Sequence 103331464
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (CA)4
Repeat start & end within CDS Repeat start: 667     Repeat end: 674
Forward primer Primer sequence:   GCTCATTCCGAACTGGCTCT     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CATTTTGATGTGTGGGGCCG     Tm(°C): 60.109     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 587     End: 886     Product size (bp): 300
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331464     NCBI Gene Symbol: LOC103331464
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   9
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11843420 ...... 11846881
CDS Sequence 103331464
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (AG)4
Repeat start & end within CDS Repeat start: 770     Repeat end: 777
Forward primer Primer sequence:   GCTCATTCCGAACTGGCTCT     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CATTTTGATGTGTGGGGCCG     Tm(°C): 60.109     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 587     End: 886     Product size (bp): 300
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331464     NCBI Gene Symbol: LOC103331464
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   9
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11843420 ...... 11846881
CDS Sequence 103331464
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAA)3
Repeat start & end within CDS Repeat start: 1459     Repeat end: 1467
Forward primer Primer sequence:   AGAAGGGTATCCAGGTCGCT     Tm(°C): 60.032     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATGGGTGTCCTCATTGCTGG     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1321     End: 1576     Product size (bp): 256
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Enzyme Id:  K15404
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331464     NCBI Gene Symbol: LOC103331464
Gene Aliases
Gene description & Other designations Description:   protein ECERIFERUM 1-like      Other designations:   protein ECERIFERUM 1-like
Chromosome, Strand & Exon count Chromosome:   LG5     Strand:   minus     Exon count:   9
Gene Location within genomic sequence Genomic accession No. NC_024130.1      Gene Start and end within genomic accession: 11843420 ...... 11846881
CDS Sequence 103331464
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AGAAGGGTATCCAGGTCGCT     Tm(°C): 60.032     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATGGGTGTCCTCATTGCTGG     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1321     End: 1576     Product size (bp): 256
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