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Statistics

Number of enzymes: 1
Total Number of designed primers: 7
Number of PGTM primers:     1
Number of PMTM primers:     6

Enzyme Id:  K15403

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327706     NCBI Gene Symbol: LOC103327706
Gene Aliases
Gene description & Other designations Description:   protein HOTHEAD      Other designations:   protein HOTHEAD
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 3396072 ...... 3400431
CDS Sequence 103327706
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AAGGG)2
Repeat start & end within CDS Repeat start: 68     Repeat end: 77
Forward primer Primer sequence:   CTTGGTTTGGGCAGTGAAGC     Tm(°C): 59.967     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GAAAGTGCTTGCACGCTTGA     Tm(°C): 59.971     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 5     End: 125     Product size (bp): 121
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Enzyme Id:  K15403

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327706     NCBI Gene Symbol: LOC103327706
Gene Aliases
Gene description & Other designations Description:   protein HOTHEAD      Other designations:   protein HOTHEAD
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 3396072 ...... 3400431
CDS Sequence 103327706
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GCTAGG)2
Repeat start & end within CDS Repeat start: 379     Repeat end: 390
Forward primer Primer sequence:   CTACTTCTGCCTCCCAAGCC     Tm(°C): 60.107     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GTGACGACGACCGAATCTGT     Tm(°C): 60.11     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 331     End: 665     Product size (bp): 335
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Enzyme Id:  K15403

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327706     NCBI Gene Symbol: LOC103327706
Gene Aliases
Gene description & Other designations Description:   protein HOTHEAD      Other designations:   protein HOTHEAD
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 3396072 ...... 3400431
CDS Sequence 103327706
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TGGGA)2
Repeat start & end within CDS Repeat start: 461     Repeat end: 470
Forward primer Primer sequence:   GCTAGGGCTAGGGTTTTGGG     Tm(°C): 60.107     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GAGGGTTCCCAGATGACAGC     Tm(°C): 60.107     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 378     End: 699     Product size (bp): 322
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Enzyme Id:  K15403

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327706     NCBI Gene Symbol: LOC103327706
Gene Aliases
Gene description & Other designations Description:   protein HOTHEAD      Other designations:   protein HOTHEAD
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 3396072 ...... 3400431
CDS Sequence 103327706
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GTC)3aca(CTG)3
Repeat start & end within CDS Repeat start: 656     Repeat end: 676
Forward primer Primer sequence:   AGGTGGCTCTAAGGGACAGT     Tm(°C): 59.883     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCACTCTGTGGCTTAGCAGC     Tm(°C): 60.037     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 544     End: 844     Product size (bp): 301
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Enzyme Id:  K15403

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327706     NCBI Gene Symbol: LOC103327706
Gene Aliases
Gene description & Other designations Description:   protein HOTHEAD      Other designations:   protein HOTHEAD
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 3396072 ...... 3400431
CDS Sequence 103327706
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CAAAG)2
Repeat start & end within CDS Repeat start: 1169     Repeat end: 1178
Forward primer Primer sequence:   TCATGTCCGCTGAGATTGGG     Tm(°C): 59.821     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TTGAAGATCGTACGGGTGGC     Tm(°C): 60.109     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1129     End: 1367     Product size (bp): 239
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Enzyme Id:  K15403

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327706     NCBI Gene Symbol: LOC103327706
Gene Aliases
Gene description & Other designations Description:   protein HOTHEAD      Other designations:   protein HOTHEAD
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 3396072 ...... 3400431
CDS Sequence 103327706
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (ATG)3
Repeat start & end within CDS Repeat start: 1699     Repeat end: 1707
Forward primer Primer sequence:   GCTCATGTAGGCACTGTGGT     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACCAGCTGCTCTTCCCAATC     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1581     End: 1760     Product size (bp): 180
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Enzyme Id:  K15403

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327706     NCBI Gene Symbol: LOC103327706
Gene Aliases
Gene description & Other designations Description:   protein HOTHEAD      Other designations:   protein HOTHEAD
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 3396072 ...... 3400431
CDS Sequence 103327706
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CTTGGTTTGGGCAGTGAAGC     Tm(°C): 59.967     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GAAAGTGCTTGCACGCTTGA     Tm(°C): 59.971     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 5     End: 125     Product size (bp): 121
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