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Statistics

Number of enzymes: 1
Total Number of designed primers: 7
Number of PGTM primers:     2
Number of PMTM primers:     5
Number of Failed designed PMTM primers: 1

Enzyme Id:  K15402
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321361     NCBI Gene Symbol: LOC103321361
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 86B1-like      Other designations:   cytochrome P450 86B1-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 14370884 ...... 14373378
CDS Sequence 103321361
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (CT)6
Repeat start & end within CDS Repeat start: 258     Repeat end: 269
Forward primer Primer sequence:   ATGATCACCCCTCCTCCTCC     Tm(°C): 60.105     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TGAAGCTGCTCAAGGAAGGG     Tm(°C): 59.963     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 30     End: 372     Product size (bp): 343
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Enzyme Id:  K15402
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321361     NCBI Gene Symbol: LOC103321361
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 86B1-like      Other designations:   cytochrome P450 86B1-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 14370884 ...... 14373378
CDS Sequence 103321361
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (AAT)3tc(TGG)3
Repeat start & end within CDS Repeat start: 952     Repeat end: 971
Forward primer Primer sequence:   GATACGCTTTGTGACCCCCA     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCCCTCCCAGCCAGTATGAA     Tm(°C): 59.956     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 797     End: 1111     Product size (bp): 315
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Enzyme Id:  K15402
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321361     NCBI Gene Symbol: LOC103321361
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 86B1-like      Other designations:   cytochrome P450 86B1-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 14370884 ...... 14373378
CDS Sequence 103321361
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (TCA)3gatcacgaccgtcgatctcatgccaacgatgg(TGA)3
Repeat start & end within CDS Repeat start: 1216     Repeat end: 1265
Forward primer Primer sequence:   TGCAGGATAGTGGGTGCAAG     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CTCAGGCTTGTAGTCCCTGC     Tm(°C): 60.108     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 1194     End: 1481     Product size (bp): 288
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Enzyme Id:  K15402
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321361     NCBI Gene Symbol: LOC103321361
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 86B1-like      Other designations:   cytochrome P450 86B1-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 14370884 ...... 14373378
CDS Sequence 103321361
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CTC)3tcattcaaacaaaatgatcacc(CCT)8acttctaatctcacct(GCCT
C)2
Repeat start & end within CDS Repeat start: 9     Repeat end: 89
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K15402
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321361     NCBI Gene Symbol: LOC103321361
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 86B1-like      Other designations:   cytochrome P450 86B1-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 14370884 ...... 14373378
CDS Sequence 103321361
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TTCCTTGTTCGAGCTGGTCC     Tm(°C): 59.965     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGGGGTCACAAAGCGTATCA     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 581     End: 815     Product size (bp): 235
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Enzyme Id:  K15402
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321926     NCBI Gene Symbol: LOC103321926
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 86B1-like      Other designations:   cytochrome P450 86B1-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 19216291 ...... 19222423
CDS Sequence 103321926
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TTTTA)2
Repeat start & end within CDS Repeat start: 951     Repeat end: 960
Forward primer Primer sequence:   CACCCAAAAGCGCTTTTCGA     Tm(°C): 59.97     GC (%): 50     Size: 20
Reverse primer Primer sequence:   GGGTAGAGCCTCAGCGATTC     Tm(°C): 59.967     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 902     End: 1189     Product size (bp): 288
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Enzyme Id:  K15402
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321926     NCBI Gene Symbol: LOC103321926
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 86B1-like      Other designations:   cytochrome P450 86B1-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 19216291 ...... 19222423
CDS Sequence 103321926
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (TG)4gggaagaagtttgcttacatgcagatgaagatggtg(GCT)3
Repeat start & end within CDS Repeat start: 1418     Repeat end: 1470
Forward primer Primer sequence:   GGGAGGTGGGGTTCTCTACT     Tm(°C): 59.957     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GCCCTAGGCTTGAGAGTCAC     Tm(°C): 59.823     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 1259     End: 1579     Product size (bp): 321
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Enzyme Id:  K15402
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321926     NCBI Gene Symbol: LOC103321926
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 86B1-like      Other designations:   cytochrome P450 86B1-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 19216291 ...... 19222423
CDS Sequence 103321926
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TCATGTTGCCGCCTTTTGTG     Tm(°C): 59.968     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TCGAAAAGCGCTTTTGGGTG     Tm(°C): 59.97     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 688     End: 921     Product size (bp): 234
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