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Statistics

Number of enzymes: 1
Total Number of designed primers: 5
Number of PGTM primers:     2
Number of PMTM primers:     3

Enzyme Id:  K15398

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103342770     NCBI Gene Symbol: LOC103342770
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 86A8      Other designations:   cytochrome P450 86A8
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103342770
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GCTTC)2
Repeat start & end within CDS Repeat start: 620     Repeat end: 629
Forward primer Primer sequence:   ATCAAGCTGAGGTTCTGCCC     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CGTTGTAGTGAGGCTTCGGT     Tm(°C): 60.039     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 462     End: 664     Product size (bp): 203
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Enzyme Id:  K15398

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103342770     NCBI Gene Symbol: LOC103342770
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 86A8      Other designations:   cytochrome P450 86A8
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103342770
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GGCTTG)2(TGGAA)2*
Repeat start & end within CDS Repeat start: 704     Repeat end: 723
Forward primer Primer sequence:   ATCAAGCTGAGGTTCTGCCC     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCAGCAACTCGAGCTTACGG     Tm(°C): 60.109     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 462     End: 801     Product size (bp): 340
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Enzyme Id:  K15398

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103342770     NCBI Gene Symbol: LOC103342770
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 86A8      Other designations:   cytochrome P450 86A8
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103342770
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TGTTTCAGAGGAAGACGGCC     Tm(°C): 59.965     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGGCAGAACCTCAGCTTGAT     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 382     End: 481     Product size (bp): 100
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Enzyme Id:  K15398

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103337535     NCBI Gene Symbol: LOC103337535
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 86A22      Other designations:   cytochrome P450 86A22
Chromosome, Strand & Exon count Chromosome:   LG7     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024132.1      Gene Start and end within genomic accession: 9137290 ...... 9139377
CDS Sequence 103337535
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AAGCC)2
Repeat start & end within CDS Repeat start: 506     Repeat end: 515
Forward primer Primer sequence:   CACTGGAATTCACCACCCGA     Tm(°C): 59.964     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GAAAGCCAACCCGCAAATGT     Tm(°C): 59.967     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 403     End: 575     Product size (bp): 173
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Enzyme Id:  K15398

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103337535     NCBI Gene Symbol: LOC103337535
Gene Aliases
Gene description & Other designations Description:   cytochrome P450 86A22      Other designations:   cytochrome P450 86A22
Chromosome, Strand & Exon count Chromosome:   LG7     Strand:   minus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_024132.1      Gene Start and end within genomic accession: 9137290 ...... 9139377
CDS Sequence 103337535
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CACGTACCAGACCTGCACTT     Tm(°C): 59.966     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GAAAGCCAACCCGCAAATGT     Tm(°C): 59.967     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 185     End: 575     Product size (bp): 391
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