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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     1
Number of PMTM primers:     3

Enzyme Id:  K14085
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340601     NCBI Gene Symbol: LOC103340601
Gene Aliases
Gene description & Other designations Description:   aldehyde dehydrogenase family 7 member A1      Other designations:   aldehyde dehydrogenase family 7 member A1
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   plus     Exon count:   15
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 13410594 ...... 13414791
CDS Sequence 103340601
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TGCAT)2
Repeat start & end within CDS Repeat start: 526     Repeat end: 535
Forward primer Primer sequence:   GGACTGAGCCGACAACTGAA     Tm(°C): 59.966     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAGCTCCCCCACAGAAAGAG     Tm(°C): 60.036     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 384     End: 675     Product size (bp): 292
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Enzyme Id:  K14085
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340601     NCBI Gene Symbol: LOC103340601
Gene Aliases
Gene description & Other designations Description:   aldehyde dehydrogenase family 7 member A1      Other designations:   aldehyde dehydrogenase family 7 member A1
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   plus     Exon count:   15
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 13410594 ...... 13414791
CDS Sequence 103340601
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (GA)4
Repeat start & end within CDS Repeat start: 1041     Repeat end: 1048
Forward primer Primer sequence:   GGGCCAGTACATACTCAGGC     Tm(°C): 59.893     GC (%): 60     Size: 20
Reverse primer Primer sequence:   ACCATGTGGCCCAATCCATT     Tm(°C): 59.957     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1017     End: 1331     Product size (bp): 315
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Enzyme Id:  K14085
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340601     NCBI Gene Symbol: LOC103340601
Gene Aliases
Gene description & Other designations Description:   aldehyde dehydrogenase family 7 member A1      Other designations:   aldehyde dehydrogenase family 7 member A1
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   plus     Exon count:   15
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 13410594 ...... 13414791
CDS Sequence 103340601
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TGAAC)2
Repeat start & end within CDS Repeat start: 1242     Repeat end: 1251
Forward primer Primer sequence:   GATCCTTACAGGCGGATCGG     Tm(°C): 60.038     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TCACTTCCTGCTTCACGACC     Tm(°C): 59.966     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1091     End: 1438     Product size (bp): 348
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Enzyme Id:  K14085
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103340601     NCBI Gene Symbol: LOC103340601
Gene Aliases
Gene description & Other designations Description:   aldehyde dehydrogenase family 7 member A1      Other designations:   aldehyde dehydrogenase family 7 member A1
Chromosome, Strand & Exon count Chromosome:   LG8     Strand:   plus     Exon count:   15
Gene Location within genomic sequence Genomic accession No. NC_024133.1      Gene Start and end within genomic accession: 13410594 ...... 13414791
CDS Sequence 103340601
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GCATCTGGGTAGGCTTGTGT     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TTCAGTTGTCGGCTCAGTCC     Tm(°C): 59.966     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 287     End: 403     Product size (bp): 117
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