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Statistics

Number of enzymes: 1
Total Number of designed primers: 2
Number of PGTM primers:     1
Number of PMTM primers:     1

Enzyme Id:  K13600

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108219296     NCBI Gene Symbol: LOC108219296
Gene Aliases DCAR_014388
Gene description & Other designations Description:   chlorophyllide a oxygenase; chloroplastic      Other designations:   chlorophyllide a oxygenase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   4     Strand:   plus     Exon count:   9
Gene Location within genomic sequence Genomic accession No. NC_030384.1      Gene Start and end within genomic accession: 22247362 ...... 22251995
CDS Sequence 108219296
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (ACAAG)2
Repeat start & end within CDS Repeat start: 1340     Repeat end: 1349
Forward primer Primer sequence:   GGAACACGGGCTCCTCTTAG     Tm(°C): 59.824     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GGGAGCAAAATCCAGGGACA     Tm(°C): 59.96     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1049     End: 1382     Product size (bp): 334
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Enzyme Id:  K13600

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108219296     NCBI Gene Symbol: LOC108219296
Gene Aliases DCAR_014388
Gene description & Other designations Description:   chlorophyllide a oxygenase; chloroplastic      Other designations:   chlorophyllide a oxygenase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   4     Strand:   plus     Exon count:   9
Gene Location within genomic sequence Genomic accession No. NC_030384.1      Gene Start and end within genomic accession: 22247362 ...... 22251995
CDS Sequence 108219296
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GGGTTGGTGCTGCTGTAGAT     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCTCGCAAGTGCCATCTGTT     Tm(°C): 59.965     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 439     End: 876     Product size (bp): 438
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