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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     2
Number of PMTM primers:     1
Number of Failed designed PMTM primers: 2

Enzyme Id:  K13600

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101261422     NCBI Gene Symbol: LOC101261422
Gene Aliases
Gene description & Other designations Description:   chlorophyllide a oxygenase; chloroplastic      Other designations:   chlorophyllide a oxygenase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   6     Strand:   minus     Exon count:   9
Gene Location within genomic sequence Genomic accession No. NC_015443.3      Gene Start and end within genomic accession: 38400173 ...... 38404124
CDS Sequence 101261422
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GATATG)2
Repeat start & end within CDS Repeat start: 951     Repeat end: 962
Forward primer Primer sequence:   TCCGGAACACATGTGCACAT     Tm(°C): 60.251     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TGTATGGGTGAAAGGCGCAT     Tm(°C): 60.035     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 769     End: 1115     Product size (bp): 347
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Enzyme Id:  K13600

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101261422     NCBI Gene Symbol: LOC101261422
Gene Aliases
Gene description & Other designations Description:   chlorophyllide a oxygenase; chloroplastic      Other designations:   chlorophyllide a oxygenase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   6     Strand:   minus     Exon count:   9
Gene Location within genomic sequence Genomic accession No. NC_015443.3      Gene Start and end within genomic accession: 38400173 ...... 38404124
CDS Sequence 101261422
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AGCAA)2
Repeat start & end within CDS Repeat start: 1581     Repeat end: 1590
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K13600

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101261422     NCBI Gene Symbol: LOC101261422
Gene Aliases
Gene description & Other designations Description:   chlorophyllide a oxygenase; chloroplastic      Other designations:   chlorophyllide a oxygenase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   6     Strand:   minus     Exon count:   9
Gene Location within genomic sequence Genomic accession No. NC_015443.3      Gene Start and end within genomic accession: 38400173 ...... 38404124
CDS Sequence 101261422
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CCTGCACGAAAAGGTTGTGG     Tm(°C): 59.969     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAGAAACCCGCACCTCAGAT     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 308     End: 453     Product size (bp): 146
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Enzyme Id:  K13600

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101244441     NCBI Gene Symbol: LOC101244441
Gene Aliases
Gene description & Other designations Description:   chlorophyllide a oxygenase; chloroplastic-like      Other designations:   chlorophyllide a oxygenase; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   11     Strand:   minus     Exon count:   9
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 5644034 ...... 5648371
CDS Sequence 101244441
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TGCTAC)2
Repeat start & end within CDS Repeat start: 12     Repeat end: 23
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K13600

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101244441     NCBI Gene Symbol: LOC101244441
Gene Aliases
Gene description & Other designations Description:   chlorophyllide a oxygenase; chloroplastic-like      Other designations:   chlorophyllide a oxygenase; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   11     Strand:   minus     Exon count:   9
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 5644034 ...... 5648371
CDS Sequence 101244441
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CCGACGGGAAGTGTGAGAAA     Tm(°C): 59.967     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGTGGATCATTTCCTGGCCA     Tm(°C): 60.033     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 865     End: 979     Product size (bp): 115
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