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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     1
Number of PMTM primers:     2
Number of Failed designed PMTM primers: 1

Enzyme Id:  K13545

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108192987     NCBI Gene Symbol: LOC108192987
Gene Aliases DCAR_021441
Gene description & Other designations Description:   red chlorophyll catabolite reductase; chloroplastic      Other designations:   red chlorophyll catabolite reductase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   6     Strand:   plus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_030386.1      Gene Start and end within genomic accession: 22926757 ...... 22929158
CDS Sequence 108192987
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (TC)4cctct(TTC)3
Repeat start & end within CDS Repeat start: 59     Repeat end: 80
Forward primer Primer sequence:   TTTCACTCCTCTCCCTCCCG     Tm(°C): 60.615     GC (%): 60     Size: 20
Reverse primer Primer sequence:   ACTCTACATCCTGCGGGAGT     Tm(°C): 60.033     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 33     End: 282     Product size (bp): 250
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Enzyme Id:  K13545

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108192987     NCBI Gene Symbol: LOC108192987
Gene Aliases DCAR_021441
Gene description & Other designations Description:   red chlorophyll catabolite reductase; chloroplastic      Other designations:   red chlorophyll catabolite reductase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   6     Strand:   plus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_030386.1      Gene Start and end within genomic accession: 22926757 ...... 22929158
CDS Sequence 108192987
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CAT)3
Repeat start & end within CDS Repeat start: 142     Repeat end: 150
Forward primer Primer sequence:   TCCTCCTCTACATCACCCGG     Tm(°C): 60.106     GC (%): 60     Size: 20
Reverse primer Primer sequence:   ACTCTACATCCTGCGGGAGT     Tm(°C): 60.033     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 111     End: 282     Product size (bp): 172
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Enzyme Id:  K13545

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108192987     NCBI Gene Symbol: LOC108192987
Gene Aliases DCAR_021441
Gene description & Other designations Description:   red chlorophyll catabolite reductase; chloroplastic      Other designations:   red chlorophyll catabolite reductase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   6     Strand:   plus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_030386.1      Gene Start and end within genomic accession: 22926757 ...... 22929158
CDS Sequence 108192987
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (AGC)3
Repeat start & end within CDS Repeat start: 915     Repeat end: 923
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K13545

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108192987     NCBI Gene Symbol: LOC108192987
Gene Aliases DCAR_021441
Gene description & Other designations Description:   red chlorophyll catabolite reductase; chloroplastic      Other designations:   red chlorophyll catabolite reductase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   6     Strand:   plus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_030386.1      Gene Start and end within genomic accession: 22926757 ...... 22929158
CDS Sequence 108192987
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TCCTCCTCTACATCACCCGG     Tm(°C): 60.106     GC (%): 60     Size: 20
Reverse primer Primer sequence:   ACTCTACATCCTGCGGGAGT     Tm(°C): 60.033     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 111     End: 282     Product size (bp): 172
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