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Statistics

Number of enzymes: 1
Total Number of designed primers: 2
Number of PGTM primers:     1
Number of PMTM primers:     1

Enzyme Id:  K13071

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108192785     NCBI Gene Symbol: LOC108192785
Gene Aliases DCAR_022680
Gene description & Other designations Description:   pheophorbide a oxygenase; chloroplastic      Other designations:   pheophorbide a oxygenase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   6     Strand:   plus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_030386.1      Gene Start and end within genomic accession: 8270438 ...... 8276503
CDS Sequence 108192785
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CATCTT)2cgtcttcat(CAA)3atcccccccaagacacta(AAG)3
Repeat start & end within CDS Repeat start: 155     Repeat end: 211
Forward primer Primer sequence:   CCCAGCCAAATCCCTCTTGT     Tm(°C): 59.96     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATCACGGTTGAGCAGCTGAA     Tm(°C): 59.965     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 61     End: 320     Product size (bp): 260
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Enzyme Id:  K13071

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108192785     NCBI Gene Symbol: LOC108192785
Gene Aliases DCAR_022680
Gene description & Other designations Description:   pheophorbide a oxygenase; chloroplastic      Other designations:   pheophorbide a oxygenase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   6     Strand:   plus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_030386.1      Gene Start and end within genomic accession: 8270438 ...... 8276503
CDS Sequence 108192785
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TTCAGCTGCTCAACCGTGAT     Tm(°C): 59.965     GC (%): 50     Size: 20
Reverse primer Primer sequence:   ATCCTCGTACAAGACCCGGA     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 301     End: 484     Product size (bp): 184
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