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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     1
Number of PMTM primers:     2

Enzyme Id:  K13071

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   543677     NCBI Gene Symbol: LOC543677
Gene Aliases
Gene description & Other designations Description:   lethal leaf spot 1-like protein      Other designations:   lethal leaf spot 1-like protein|LLS1-like protein
Chromosome, Strand & Exon count Chromosome:   11     Strand:   minus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 52495422 ...... 52499145
CDS Sequence 543677
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CAA)4cagctact(GAA)3(AGAAG)2*
Repeat start & end within CDS Repeat start: 152     Repeat end: 186
Forward primer Primer sequence:   TCCTCTTAGAGTAGCTGCACCT     Tm(°C): 60.025     GC (%): 50     Size: 22
Reverse primer Primer sequence:   TCGGGTCGAGATCTTCCACT     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 128     End: 303     Product size (bp): 176
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Enzyme Id:  K13071

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   543677     NCBI Gene Symbol: LOC543677
Gene Aliases
Gene description & Other designations Description:   lethal leaf spot 1-like protein      Other designations:   lethal leaf spot 1-like protein|LLS1-like protein
Chromosome, Strand & Exon count Chromosome:   11     Strand:   minus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 52495422 ...... 52499145
CDS Sequence 543677
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TCG)3
Repeat start & end within CDS Repeat start: 238     Repeat end: 246
Forward primer Primer sequence:   GCTGCACCTCCAACAACAAC     Tm(°C): 60.249     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCGGGTCGAGATCTTCCACT     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 141     End: 303     Product size (bp): 163
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Enzyme Id:  K13071

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   543677     NCBI Gene Symbol: LOC543677
Gene Aliases
Gene description & Other designations Description:   lethal leaf spot 1-like protein      Other designations:   lethal leaf spot 1-like protein|LLS1-like protein
Chromosome, Strand & Exon count Chromosome:   11     Strand:   minus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 52495422 ...... 52499145
CDS Sequence 543677
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AGATGGAGGCATCTGGACCT     Tm(°C): 60.03     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CTGGTCTTTCCTGGTGCCAT     Tm(°C): 59.961     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 823     End: 1015     Product size (bp): 193
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