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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     2
Number of PMTM primers:     1

Enzyme Id:  K12873

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101250862     NCBI Gene Symbol: LOC101250862
Gene Aliases
Gene description & Other designations Description:   protein BUD31 homolog-like      Other designations:   protein BUD31 homolog 1|protein BUD31 homolog-like
Chromosome, Strand & Exon count Chromosome:   11     Strand:   minus     Exon count:   4
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 53073625 ...... 53078308
CDS Sequence 101250862
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CGTGT)2
Repeat start & end within CDS Repeat start: 353     Repeat end: 362
Forward primer Primer sequence:   AGGTTGCACATCAGAGGAGC     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAGCAGTTTCCCTGAGGTGT     Tm(°C): 59.891     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 151     End: 387     Product size (bp): 237
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Enzyme Id:  K12873

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101250862     NCBI Gene Symbol: LOC101250862
Gene Aliases
Gene description & Other designations Description:   protein BUD31 homolog-like      Other designations:   protein BUD31 homolog 1|protein BUD31 homolog-like
Chromosome, Strand & Exon count Chromosome:   11     Strand:   minus     Exon count:   4
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 53073625 ...... 53078308
CDS Sequence 101250862
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AGGTTGCACATCAGAGGAGC     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACACGACACGCACAAGTAGT     Tm(°C): 59.898     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 151     End: 361     Product size (bp): 211
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Enzyme Id:  K12873

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101264965     NCBI Gene Symbol: LOC101264965
Gene Aliases
Gene description & Other designations Description:   protein BUD31 homolog 2      Other designations:   protein BUD31 homolog 2
Chromosome, Strand & Exon count Chromosome:   9     Strand:   minus     Exon count:   4
Gene Location within genomic sequence Genomic accession No. NC_015446.3      Gene Start and end within genomic accession: 2673488 ...... 2676773
CDS Sequence 101264965
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GCTGTTTGAGGTGCATGCAA     Tm(°C): 59.968     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TACAGCCGCAATGGACACAT     Tm(°C): 60.036     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 301     End: 414     Product size (bp): 114
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