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Statistics

Number of enzymes: 1
Total Number of designed primers: 8
Number of PGTM primers:     1
Number of PMTM primers:     7

Enzyme Id:  K12855

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265451     NCBI Gene Symbol: LOC101265451
Gene Aliases
Gene description & Other designations Description:   protein STABILIZED1      Other designations:   protein STABILIZED1
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 62685685 ...... 62689052
CDS Sequence 101265451
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (AACCCT)2agaaattaatccttttgtaacttctcttca(AACCCT)2
Repeat start & end within CDS Repeat start: 30     Repeat end: 83
Forward primer Primer sequence:   TGGTGTTCATCAATCTTCCGA     Tm(°C): 57.306     GC (%): 42.857     Size: 21
Reverse primer Primer sequence:   ACAGTGCTTCCACATCGAGG     Tm(°C): 60.037     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1     End: 168     Product size (bp): 168
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Enzyme Id:  K12855

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265451     NCBI Gene Symbol: LOC101265451
Gene Aliases
Gene description & Other designations Description:   protein STABILIZED1      Other designations:   protein STABILIZED1
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 62685685 ...... 62689052
CDS Sequence 101265451
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (ACTCTA)2
Repeat start & end within CDS Repeat start: 199     Repeat end: 210
Forward primer Primer sequence:   CCTCGATGTGGAAGCACTGT     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CACGAGCCGGACCAATATCA     Tm(°C): 59.896     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 149     End: 360     Product size (bp): 212
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Enzyme Id:  K12855

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265451     NCBI Gene Symbol: LOC101265451
Gene Aliases
Gene description & Other designations Description:   protein STABILIZED1      Other designations:   protein STABILIZED1
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 62685685 ...... 62689052
CDS Sequence 101265451
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TGGGT)2
Repeat start & end within CDS Repeat start: 303     Repeat end: 312
Forward primer Primer sequence:   CCTCGATGTGGAAGCACTGT     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CACGAGCCGGACCAATATCA     Tm(°C): 59.896     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 149     End: 360     Product size (bp): 212
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Enzyme Id:  K12855

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265451     NCBI Gene Symbol: LOC101265451
Gene Aliases
Gene description & Other designations Description:   protein STABILIZED1      Other designations:   protein STABILIZED1
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 62685685 ...... 62689052
CDS Sequence 101265451
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TGGACG)2
Repeat start & end within CDS Repeat start: 417     Repeat end: 428
Forward primer Primer sequence:   CCTCGATGTGGAAGCACTGT     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CTTTCTCCTCCGCATCCTCC     Tm(°C): 59.894     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 149     End: 477     Product size (bp): 329
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Enzyme Id:  K12855

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265451     NCBI Gene Symbol: LOC101265451
Gene Aliases
Gene description & Other designations Description:   protein STABILIZED1      Other designations:   protein STABILIZED1
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 62685685 ...... 62689052
CDS Sequence 101265451
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (AAG)3
Repeat start & end within CDS Repeat start: 778     Repeat end: 786
Forward primer Primer sequence:   GATGTGGGGTTGTTTGCGTC     Tm(°C): 60.04     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACGTGCTCTTTTTCCTGCCT     Tm(°C): 60.179     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 519     End: 853     Product size (bp): 335
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Enzyme Id:  K12855

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265451     NCBI Gene Symbol: LOC101265451
Gene Aliases
Gene description & Other designations Description:   protein STABILIZED1      Other designations:   protein STABILIZED1
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 62685685 ...... 62689052
CDS Sequence 101265451
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TGCTGA)2
Repeat start & end within CDS Repeat start: 1842     Repeat end: 1853
Forward primer Primer sequence:   GGGCTTTGCAGAGAGAAGGT     Tm(°C): 59.963     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCAAGTGATTCCCTCGTGCC     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1684     End: 1990     Product size (bp): 307
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Enzyme Id:  K12855

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265451     NCBI Gene Symbol: LOC101265451
Gene Aliases
Gene description & Other designations Description:   protein STABILIZED1      Other designations:   protein STABILIZED1
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 62685685 ...... 62689052
CDS Sequence 101265451
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GA)4acttggaaatgtggat(GAGGA)2
Repeat start & end within CDS Repeat start: 2263     Repeat end: 2296
Forward primer Primer sequence:   TTGGAGCGGGTTTGGATGAA     Tm(°C): 59.889     GC (%): 50     Size: 20
Reverse primer Primer sequence:   AGAACTGCTCGAGCTTTGCT     Tm(°C): 59.965     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 2229     End: 2506     Product size (bp): 278
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Enzyme Id:  K12855

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265451     NCBI Gene Symbol: LOC101265451
Gene Aliases
Gene description & Other designations Description:   protein STABILIZED1      Other designations:   protein STABILIZED1
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 62685685 ...... 62689052
CDS Sequence 101265451
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AGCAAAGCTCGAGCAGTTCT     Tm(°C): 59.965     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CACATCGCTTCAGCACATCG     Tm(°C): 59.974     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 2487     End: 2913     Product size (bp): 427
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