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Statistics

Number of enzymes: 1
Total Number of designed primers: 7
Number of PGTM primers:     1
Number of PMTM primers:     6
Number of Failed designed PMTM primers: 1

Enzyme Id:  K12852

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101252197     NCBI Gene Symbol: LOC101252197
Gene Aliases
Gene description & Other designations Description:   110 kDa U5 small nuclear ribonucleoprotein component CLO      Other designations:   110 kDa U5 small nuclear ribonucleoprotein component CLO
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 1950568 ...... 1956946
CDS Sequence 101252197
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GATGAA)2
Repeat start & end within CDS Repeat start: 277     Repeat end: 288
Forward primer Primer sequence:   ATGGATGGCTTGCCACTCAA     Tm(°C): 59.96     GC (%): 50     Size: 20
Reverse primer Primer sequence:   GGTGTGTTTGTTCCACCAGC     Tm(°C): 59.898     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 154     End: 489     Product size (bp): 336
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Enzyme Id:  K12852

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101252197     NCBI Gene Symbol: LOC101252197
Gene Aliases
Gene description & Other designations Description:   110 kDa U5 small nuclear ribonucleoprotein component CLO      Other designations:   110 kDa U5 small nuclear ribonucleoprotein component CLO
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 1950568 ...... 1956946
CDS Sequence 101252197
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TGA)3
Repeat start & end within CDS Repeat start: 1590     Repeat end: 1598
Forward primer Primer sequence:   GCTGCAGCTAGGAAAGTGGA     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATCAGAACCCATGCACCTGG     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1371     End: 1696     Product size (bp): 326
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Enzyme Id:  K12852

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101252197     NCBI Gene Symbol: LOC101252197
Gene Aliases
Gene description & Other designations Description:   110 kDa U5 small nuclear ribonucleoprotein component CLO      Other designations:   110 kDa U5 small nuclear ribonucleoprotein component CLO
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 1950568 ...... 1956946
CDS Sequence 101252197
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AAAAT)2
Repeat start & end within CDS Repeat start: 2087     Repeat end: 2096
Forward primer Primer sequence:   GTGGAAGTGAAGGTGGCTGA     Tm(°C): 59.891     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATGCCCAAATAGACCGTGCA     Tm(°C): 60.035     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1992     End: 2244     Product size (bp): 253
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Enzyme Id:  K12852

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101252197     NCBI Gene Symbol: LOC101252197
Gene Aliases
Gene description & Other designations Description:   110 kDa U5 small nuclear ribonucleoprotein component CLO      Other designations:   110 kDa U5 small nuclear ribonucleoprotein component CLO
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 1950568 ...... 1956946
CDS Sequence 101252197
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CCAAA)2
Repeat start & end within CDS Repeat start: 2196     Repeat end: 2205
Forward primer Primer sequence:   TTGTGAGCATCGATTGGCCT     Tm(°C): 60.035     GC (%): 50     Size: 20
Reverse primer Primer sequence:   ATGCCCAAATAGACCGTGCA     Tm(°C): 60.035     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 2152     End: 2244     Product size (bp): 93
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Enzyme Id:  K12852

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101252197     NCBI Gene Symbol: LOC101252197
Gene Aliases
Gene description & Other designations Description:   110 kDa U5 small nuclear ribonucleoprotein component CLO      Other designations:   110 kDa U5 small nuclear ribonucleoprotein component CLO
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 1950568 ...... 1956946
CDS Sequence 101252197
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AAGTG)2
Repeat start & end within CDS Repeat start: 2295     Repeat end: 2304
Forward primer Primer sequence:   TGCACGGTCTATTTGGGCAT     Tm(°C): 60.035     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TCATCGCAAAGGGGTCCTTC     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 2225     End: 2386     Product size (bp): 162
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Enzyme Id:  K12852

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101252197     NCBI Gene Symbol: LOC101252197
Gene Aliases
Gene description & Other designations Description:   110 kDa U5 small nuclear ribonucleoprotein component CLO      Other designations:   110 kDa U5 small nuclear ribonucleoprotein component CLO
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 1950568 ...... 1956946
CDS Sequence 101252197
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TCCAA)2
Repeat start & end within CDS Repeat start: 2814     Repeat end: 2823
Forward primer Primer sequence:   CCAAACGCCCATGGATTGTC     Tm(°C): 59.827     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACCATGAACTCACGAGCCAG     Tm(°C): 60.037     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 2549     End: 2845     Product size (bp): 297
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Enzyme Id:  K12852

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101252197     NCBI Gene Symbol: LOC101252197
Gene Aliases
Gene description & Other designations Description:   110 kDa U5 small nuclear ribonucleoprotein component CLO      Other designations:   110 kDa U5 small nuclear ribonucleoprotein component CLO
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 1950568 ...... 1956946
CDS Sequence 101252197
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GGAAGA)2cgatgagctgcctgataggag(TGA)3gagagctgtatc(TGA)3
Repeat start & end within CDS Repeat start: 75     Repeat end: 137
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K12852

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101252197     NCBI Gene Symbol: LOC101252197
Gene Aliases
Gene description & Other designations Description:   110 kDa U5 small nuclear ribonucleoprotein component CLO      Other designations:   110 kDa U5 small nuclear ribonucleoprotein component CLO
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 1950568 ...... 1956946
CDS Sequence 101252197
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CCAGGTGCATGGGTTCTGAT     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGGCCAATCGATGCTCACAA     Tm(°C): 60.035     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1677     End: 2171     Product size (bp): 495
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