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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     1
Number of PMTM primers:     3

Enzyme Id:  K12850

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101266210     NCBI Gene Symbol: LOC101266210
Gene Aliases
Gene description & Other designations Description:   pre-mRNA splicing factor SR-like 1      Other designations:   pre-mRNA splicing factor SR-like 1|pre-mRNA-splicing factor 38B
Chromosome, Strand & Exon count Chromosome:   4     Strand:   plus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_015441.3      Gene Start and end within genomic accession: 4824642 ...... 4832773
CDS Sequence 101266210
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TTCCTG)2
Repeat start & end within CDS Repeat start: 473     Repeat end: 484
Forward primer Primer sequence:   TCTCTCCTGGATCCAGTGGG     Tm(°C): 60.032     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TTTCGAGACTGGCAACTGCT     Tm(°C): 59.893     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 376     End: 513     Product size (bp): 138
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Enzyme Id:  K12850

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101266210     NCBI Gene Symbol: LOC101266210
Gene Aliases
Gene description & Other designations Description:   pre-mRNA splicing factor SR-like 1      Other designations:   pre-mRNA splicing factor SR-like 1|pre-mRNA-splicing factor 38B
Chromosome, Strand & Exon count Chromosome:   4     Strand:   plus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_015441.3      Gene Start and end within genomic accession: 4824642 ...... 4832773
CDS Sequence 101266210
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CCGGGA)2(CAGGGA)6aagaactagggacagagaacgt(GATAGG)4tatagg
gaccaggaaagagaaagggataggggcagggatcgg(GATAGA)2
Repeat start & end within CDS Repeat start: 798     Repeat end: 945
Forward primer Primer sequence:   ATGGTTCAAGACGTTCCCCC     Tm(°C): 59.962     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GTCTCTATCCCTTCCCCGGT     Tm(°C): 60.106     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 715     End: 1025     Product size (bp): 311
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Enzyme Id:  K12850

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101266210     NCBI Gene Symbol: LOC101266210
Gene Aliases
Gene description & Other designations Description:   pre-mRNA splicing factor SR-like 1      Other designations:   pre-mRNA splicing factor SR-like 1|pre-mRNA-splicing factor 38B
Chromosome, Strand & Exon count Chromosome:   4     Strand:   plus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_015441.3      Gene Start and end within genomic accession: 4824642 ...... 4832773
CDS Sequence 101266210
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (CAGGAG)2
Repeat start & end within CDS Repeat start: 1080     Repeat end: 1091
Forward primer Primer sequence:   AAAGGGATAGGGGCAGGGAT     Tm(°C): 60.028     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATCCCTAGGTGGAGTTCGCT     Tm(°C): 60.032     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 910     End: 1172     Product size (bp): 263
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Enzyme Id:  K12850

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101266210     NCBI Gene Symbol: LOC101266210
Gene Aliases
Gene description & Other designations Description:   pre-mRNA splicing factor SR-like 1      Other designations:   pre-mRNA splicing factor SR-like 1|pre-mRNA-splicing factor 38B
Chromosome, Strand & Exon count Chromosome:   4     Strand:   plus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_015441.3      Gene Start and end within genomic accession: 4824642 ...... 4832773
CDS Sequence 101266210
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AAAGGGATAGGGGCAGGGAT     Tm(°C): 60.028     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATCCCTAGGTGGAGTTCGCT     Tm(°C): 60.032     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 910     End: 1172     Product size (bp): 263
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