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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     1
Number of PMTM primers:     2

Enzyme Id:  K12848

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101244419     NCBI Gene Symbol: LOC101244419
Gene Aliases
Gene description & Other designations Description:   zinc finger matrin-type protein 2      Other designations:   zinc finger matrin-type protein 2
Chromosome, Strand & Exon count Chromosome:   1     Strand:   plus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_015438.3      Gene Start and end within genomic accession: 90272291 ...... 90278544
CDS Sequence 101244419
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AGAGA)2
Repeat start & end within CDS Repeat start: 61     Repeat end: 70
Forward primer Primer sequence:   CAGCAATCATGTTGTTGGAGT     Tm(°C): 57.071     GC (%): 42.857     Size: 21
Reverse primer Primer sequence:   TCTCTCCACCCGCATAGACA     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 11     End: 359     Product size (bp): 349
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Enzyme Id:  K12848

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101244419     NCBI Gene Symbol: LOC101244419
Gene Aliases
Gene description & Other designations Description:   zinc finger matrin-type protein 2      Other designations:   zinc finger matrin-type protein 2
Chromosome, Strand & Exon count Chromosome:   1     Strand:   plus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_015438.3      Gene Start and end within genomic accession: 90272291 ...... 90278544
CDS Sequence 101244419
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CAG)3(AGGAAG)2*aaaggaaacgtttacgtcgagaaaagaagaaag(AGA)3
Repeat start & end within CDS Repeat start: 460     Repeat end: 520
Forward primer Primer sequence:   TGTCTATGCGGGTGGAGAGA     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCCGAATCCCATCATAGCTGC     Tm(°C): 59.998     GC (%): 52.381     Size: 21
Primer start, end within sequence and product size Start: 340     End: 584     Product size (bp): 245
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Enzyme Id:  K12848

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101244419     NCBI Gene Symbol: LOC101244419
Gene Aliases
Gene description & Other designations Description:   zinc finger matrin-type protein 2      Other designations:   zinc finger matrin-type protein 2
Chromosome, Strand & Exon count Chromosome:   1     Strand:   plus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_015438.3      Gene Start and end within genomic accession: 90272291 ...... 90278544
CDS Sequence 101244419
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GAAGAGGAGGCTGATGGTCG     Tm(°C): 59.896     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TCTCTCCACCCGCATAGACA     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 96     End: 359     Product size (bp): 264
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