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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     1
Number of PMTM primers:     3

Enzyme Id:  K12846
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265655     NCBI Gene Symbol: LOC101265655
Gene Aliases
Gene description & Other designations Description:   U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein      Other designations:   U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein
Chromosome, Strand & Exon count Chromosome:   7     Strand:   minus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 8632083 ...... 8640204
CDS Sequence 101265655
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GAGACC)4
Repeat start & end within CDS Repeat start: 29     Repeat end: 52
Forward primer Primer sequence:   CGGACCGTGATCGAACTCG     Tm(°C): 60.583     GC (%): 63.158     Size: 19
Reverse primer Primer sequence:   TTGTCCCTTTCTCGGTCACG     Tm(°C): 59.967     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 4     End: 94     Product size (bp): 91
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Enzyme Id:  K12846
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265655     NCBI Gene Symbol: LOC101265655
Gene Aliases
Gene description & Other designations Description:   U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein      Other designations:   U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein
Chromosome, Strand & Exon count Chromosome:   7     Strand:   minus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 8632083 ...... 8640204
CDS Sequence 101265655
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (CACCGC)2
Repeat start & end within CDS Repeat start: 229     Repeat end: 240
Forward primer Primer sequence:   GCATACTCGCTCCCGTACTC     Tm(°C): 60.04     GC (%): 60     Size: 20
Reverse primer Primer sequence:   CCCCACTCCCACTCACATTC     Tm(°C): 60.035     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 188     End: 483     Product size (bp): 296
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Enzyme Id:  K12846
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265655     NCBI Gene Symbol: LOC101265655
Gene Aliases
Gene description & Other designations Description:   U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein      Other designations:   U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein
Chromosome, Strand & Exon count Chromosome:   7     Strand:   minus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 8632083 ...... 8640204
CDS Sequence 101265655
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (AGGGAC)2agacgcagagaagtttcggattttgtggatgggatagccaaagaac
(AACAGA)2agaaaaaga(ACAAGG)2agaatgt(GAGTGG)2gggtgttggtga
aggaggagaagagatgatggatgaaga(TGAGAT)2
Repeat start & end within CDS Repeat start: 373     Repeat end: 533
Forward primer Primer sequence:   CCCTCAGCTGAAAGTCCTCC     Tm(°C): 59.748     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GCTGGCGTTTGGTGACTTTT     Tm(°C): 59.898     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 306     End: 633     Product size (bp): 328
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Enzyme Id:  K12846
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265655     NCBI Gene Symbol: LOC101265655
Gene Aliases
Gene description & Other designations Description:   U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein      Other designations:   U4/U6.U5 small nuclear ribonucleoprotein 27 kDa protein
Chromosome, Strand & Exon count Chromosome:   7     Strand:   minus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 8632083 ...... 8640204
CDS Sequence 101265655
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CGTGACCGAGAAAGGGACAA     Tm(°C): 59.967     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCCCACTCCCACTCACATTC     Tm(°C): 60.035     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 75     End: 483     Product size (bp): 409
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