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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     2
Number of PMTM primers:     2

Enzyme Id:  K12844

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101243803     NCBI Gene Symbol: LOC101243803
Gene Aliases
Gene description & Other designations Description:   U4/U6 small nuclear ribonucleoprotein Prp31 homolog      Other designations:   U4/U6 small nuclear ribonucleoprotein Prp31 homolog
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 66490355 ...... 66492429
CDS Sequence 101243803
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAA)3
Repeat start & end within CDS Repeat start: 144     Repeat end: 152
Forward primer Primer sequence:   GGCGTCAACTACTAGTGGCA     Tm(°C): 59.754     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACCACCCAGACTCCTCACAT     Tm(°C): 60.179     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 53     End: 305     Product size (bp): 253
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Enzyme Id:  K12844

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101243803     NCBI Gene Symbol: LOC101243803
Gene Aliases
Gene description & Other designations Description:   U4/U6 small nuclear ribonucleoprotein Prp31 homolog      Other designations:   U4/U6 small nuclear ribonucleoprotein Prp31 homolog
Chromosome, Strand & Exon count Chromosome:   7     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 66490355 ...... 66492429
CDS Sequence 101243803
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AAGCAGAGAGGTGGTGTTCG     Tm(°C): 59.966     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAGCCTGACCAAGCATACCA     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 579     End: 732     Product size (bp): 154
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Enzyme Id:  K12844

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101261054     NCBI Gene Symbol: LOC101261054
Gene Aliases
Gene description & Other designations Description:   U4/U6 small nuclear ribonucleoprotein Prp31      Other designations:   U4/U6 small nuclear ribonucleoprotein Prp31
Chromosome, Strand & Exon count Chromosome:   8     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_015445.3      Gene Start and end within genomic accession: 62360782 ...... 62364892
CDS Sequence 101261054
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GAAAT)2
Repeat start & end within CDS Repeat start: 434     Repeat end: 443
Forward primer Primer sequence:   ACCCGATTGATTATGCCCGT     Tm(°C): 59.528     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TGCACTCCCAACTACAGCAG     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 397     End: 677     Product size (bp): 281
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Enzyme Id:  K12844

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101261054     NCBI Gene Symbol: LOC101261054
Gene Aliases
Gene description & Other designations Description:   U4/U6 small nuclear ribonucleoprotein Prp31      Other designations:   U4/U6 small nuclear ribonucleoprotein Prp31
Chromosome, Strand & Exon count Chromosome:   8     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_015445.3      Gene Start and end within genomic accession: 62360782 ...... 62364892
CDS Sequence 101261054
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TGCTGTAGTTGGGAGTGCAG     Tm(°C): 59.965     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGATCGAGTCAACACGTGCA     Tm(°C): 59.969     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 659     End: 924     Product size (bp): 266
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