PlantPathMarks Database

Statistics

Number of enzymes: 1

Total Number of designed primers: 20

Number of PGTM primers: 2

Number of PMTM primers: 18

Number of Failed designed PMTM primers: 2

Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101255099 NCBI Gene Symbol: LOC101255099
Gene Aliases
Gene description & Other designations	Description: protein RDM16 Other designations: protein RDM16\|U4/U6 small nuclear ribonucleoprotein Prp3
Chromosome, Strand & Exon count	Chromosome: 5 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015442.3 Gene Start and end within genomic accession: 65520331 ...... 65525115
CDS Sequence	101255099
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Penta Repeat sequence: (AAGAA)2
Repeat start & end within CDS	Repeat start: 485 Repeat end: 494
Forward primer	Primer sequence: ACCTGAGAGTTGGCGATGTG Tm(°C): 60.037 GC (%): 55 Size: 20
Reverse primer	Primer sequence: CCTGTCCATCGTCATAGGGC Tm(°C): 59.966 GC (%): 60 Size: 20
Primer start, end within sequence and product size	Start: 430 End: 561 Product size (bp): 132
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101255099 NCBI Gene Symbol: LOC101255099
Gene Aliases
Gene description & Other designations	Description: protein RDM16 Other designations: protein RDM16\|U4/U6 small nuclear ribonucleoprotein Prp3
Chromosome, Strand & Exon count	Chromosome: 5 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015442.3 Gene Start and end within genomic accession: 65520331 ...... 65525115
CDS Sequence	101255099
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Tri Repeat sequence: (CCA)3
Repeat start & end within CDS	Repeat start: 961 Repeat end: 969
Forward primer	Primer sequence: ACAAGGGTGCCAACTCTACG Tm(°C): 59.965 GC (%): 55 Size: 20
Reverse primer	Primer sequence: CATGAGGCAAGCTACTCGGT Tm(°C): 59.823 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 871 End: 1077 Product size (bp): 207
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101255099 NCBI Gene Symbol: LOC101255099
Gene Aliases
Gene description & Other designations	Description: protein RDM16 Other designations: protein RDM16\|U4/U6 small nuclear ribonucleoprotein Prp3
Chromosome, Strand & Exon count	Chromosome: 5 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015442.3 Gene Start and end within genomic accession: 65520331 ...... 65525115
CDS Sequence	101255099
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Penta Repeat sequence: (CACAG)2
Repeat start & end within CDS	Repeat start: 1089 Repeat end: 1098
Forward primer	Primer sequence: ACAAGGGTGCCAACTCTACG Tm(°C): 59.965 GC (%): 55 Size: 20
Reverse primer	Primer sequence: CGCACGCTTTATCGCTTCAT Tm(°C): 59.698 GC (%): 50 Size: 20
Primer start, end within sequence and product size	Start: 871 End: 1121 Product size (bp): 251
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101255099 NCBI Gene Symbol: LOC101255099
Gene Aliases
Gene description & Other designations	Description: protein RDM16 Other designations: protein RDM16\|U4/U6 small nuclear ribonucleoprotein Prp3
Chromosome, Strand & Exon count	Chromosome: 5 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015442.3 Gene Start and end within genomic accession: 65520331 ...... 65525115
CDS Sequence	101255099
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Hexa Repeat sequence: (GGCAAA)2
Repeat start & end within CDS	Repeat start: 1575 Repeat end: 1586
Forward primer	Primer sequence: TGATCCGAGGATGGGCATTG Tm(°C): 59.891 GC (%): 55 Size: 20
Reverse primer	Primer sequence: GAGCTTCCCACCACTCAACA Tm(°C): 59.891 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 1409 End: 1689 Product size (bp): 281
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101255099 NCBI Gene Symbol: LOC101255099
Gene Aliases
Gene description & Other designations	Description: protein RDM16 Other designations: protein RDM16\|U4/U6 small nuclear ribonucleoprotein Prp3
Chromosome, Strand & Exon count	Chromosome: 5 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015442.3 Gene Start and end within genomic accession: 65520331 ...... 65525115
CDS Sequence	101255099
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Penta Repeat sequence: (TAACT)2
Repeat start & end within CDS	Repeat start: 1731 Repeat end: 1740
Forward primer	Primer sequence: TGTTGAGTGGTGGGAAGCTC Tm(°C): 59.891 GC (%): 55 Size: 20
Reverse primer	Primer sequence: TTTGTGGGATCCTGAGTGGC Tm(°C): 59.962 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 1670 End: 2008 Product size (bp): 339
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101255099 NCBI Gene Symbol: LOC101255099
Gene Aliases
Gene description & Other designations	Description: protein RDM16 Other designations: protein RDM16\|U4/U6 small nuclear ribonucleoprotein Prp3
Chromosome, Strand & Exon count	Chromosome: 5 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015442.3 Gene Start and end within genomic accession: 65520331 ...... 65525115
CDS Sequence	101255099
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Compound Repeat sequence: (GA)4aaagcaggaaatgatacgtcagggcctgttagaacc(ACCAAA)2
Repeat start & end within CDS	Repeat start: 1894 Repeat end: 1949
Forward primer	Primer sequence: GGAACACCCCCGTCCTATTG Tm(°C): 60.107 GC (%): 60 Size: 20
Reverse primer	Primer sequence: TTTGTGGGATCCTGAGTGGC Tm(°C): 59.962 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 1778 End: 2008 Product size (bp): 231
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101255099 NCBI Gene Symbol: LOC101255099
Gene Aliases
Gene description & Other designations	Description: protein RDM16 Other designations: protein RDM16\|U4/U6 small nuclear ribonucleoprotein Prp3
Chromosome, Strand & Exon count	Chromosome: 5 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015442.3 Gene Start and end within genomic accession: 65520331 ...... 65525115
CDS Sequence	101255099
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Tri Repeat sequence: (TGC)3
Repeat start & end within CDS	Repeat start: 2031 Repeat end: 2039
Forward primer	Primer sequence: GCCACTCAGGATCCCACAAA Tm(°C): 59.962 GC (%): 55 Size: 20
Reverse primer	Primer sequence: TCCCAAGATAACCGCACACC Tm(°C): 60.036 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 1989 End: 2261 Product size (bp): 273
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101255099 NCBI Gene Symbol: LOC101255099
Gene Aliases
Gene description & Other designations	Description: protein RDM16 Other designations: protein RDM16\|U4/U6 small nuclear ribonucleoprotein Prp3
Chromosome, Strand & Exon count	Chromosome: 5 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015442.3 Gene Start and end within genomic accession: 65520331 ...... 65525115
CDS Sequence	101255099
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Penta Repeat sequence: (AAAGA)2
Repeat start & end within CDS	Repeat start: 2110 Repeat end: 2119
Forward primer	Primer sequence: GCCACTCAGGATCCCACAAA Tm(°C): 59.962 GC (%): 55 Size: 20
Reverse primer	Primer sequence: TCCCAAGATAACCGCACACC Tm(°C): 60.036 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 1989 End: 2261 Product size (bp): 273
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101255099 NCBI Gene Symbol: LOC101255099
Gene Aliases
Gene description & Other designations	Description: protein RDM16 Other designations: protein RDM16\|U4/U6 small nuclear ribonucleoprotein Prp3
Chromosome, Strand & Exon count	Chromosome: 5 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015442.3 Gene Start and end within genomic accession: 65520331 ...... 65525115
CDS Sequence	101255099
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Compound Repeat sequence: (GCT)3gttaagaaagaagacgatgac(GAAGAT)2aagcctctcaacaa(GT)4
Repeat start & end within CDS	Repeat start: 2344 Repeat end: 2407
Forward primer	Primer sequence: GGTGTGCGGTTATCTTGGGA Tm(°C): 60.036 GC (%): 55 Size: 20
Reverse primer	Primer sequence: TCAGTCCTGCACTCGTGAAC Tm(°C): 59.968 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 2242 End: 2473 Product size (bp): 232
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101255099 NCBI Gene Symbol: LOC101255099
Gene Aliases
Gene description & Other designations	Description: protein RDM16 Other designations: protein RDM16\|U4/U6 small nuclear ribonucleoprotein Prp3
Chromosome, Strand & Exon count	Chromosome: 5 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015442.3 Gene Start and end within genomic accession: 65520331 ...... 65525115
CDS Sequence	101255099
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Tri Repeat sequence: (GCT)3
Repeat start & end within CDS	Repeat start: 2476 Repeat end: 2484
Forward primer	Primer sequence: GGTGTGCGGTTATCTTGGGA Tm(°C): 60.036 GC (%): 55 Size: 20
Reverse primer	Primer sequence: AACCCCAGCATCAGCAAAGA Tm(°C): 59.887 GC (%): 50 Size: 20
Primer start, end within sequence and product size	Start: 2242 End: 2510 Product size (bp): 269
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101255099 NCBI Gene Symbol: LOC101255099
Gene Aliases
Gene description & Other designations	Description: protein RDM16 Other designations: protein RDM16\|U4/U6 small nuclear ribonucleoprotein Prp3
Chromosome, Strand & Exon count	Chromosome: 5 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015442.3 Gene Start and end within genomic accession: 65520331 ...... 65525115
CDS Sequence	101255099
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Compound Repeat sequence: (GA)4aaaatcgagtaagagagaaagaggatccagggacgg(CGA)4
Repeat start & end within CDS	Repeat start: 4 Repeat end: 59
Forward primer	Primer sequence: None Tm(°C): GC (%): Size:
Reverse primer	Primer sequence: None Tm(°C): GC (%): Size:
Primer start, end within sequence and product size	Start: End: Product size (bp):
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101255099 NCBI Gene Symbol: LOC101255099
Gene Aliases
Gene description & Other designations	Description: protein RDM16 Other designations: protein RDM16\|U4/U6 small nuclear ribonucleoprotein Prp3
Chromosome, Strand & Exon count	Chromosome: 5 Strand: plus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015442.3 Gene Start and end within genomic accession: 65520331 ...... 65525115
CDS Sequence	101255099
	Marker Information
Marker Type	PGTM
Repeat type & sequence	Repeat type: Repeat sequence:
Repeat start & end within CDS	Repeat start: Repeat end:
Forward primer	Primer sequence: CACGATCGCGAGGATGAGAA Tm(°C): 59.971 GC (%): 55 Size: 20
Reverse primer	Primer sequence: CCTGTCCATCGTCATAGGGC Tm(°C): 59.966 GC (%): 60 Size: 20
Primer start, end within sequence and product size	Start: 162 End: 561 Product size (bp): 400
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101258563 NCBI Gene Symbol: LOC101258563
Gene Aliases
Gene description & Other designations	Description: protein RDM16-like Other designations: protein RDM16-like
Chromosome, Strand & Exon count	Chromosome: 6 Strand: minus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015443.3 Gene Start and end within genomic accession: 26773771 ...... 26786689
CDS Sequence	101258563
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Compound Repeat sequence: (TCA)3ccatcgttccaagcatcggtcggaagacggacatcaccgatct(GA)4
Repeat start & end within CDS	Repeat start: 84 Repeat end: 143
Forward primer	Primer sequence: CAAACACCGCAGTTCCAAGG Tm(°C): 59.969 GC (%): 55 Size: 20
Reverse primer	Primer sequence: CCTACCATTGTCGTCCTCGG Tm(°C): 59.898 GC (%): 60 Size: 20
Primer start, end within sequence and product size	Start: 38 End: 308 Product size (bp): 271
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101258563 NCBI Gene Symbol: LOC101258563
Gene Aliases
Gene description & Other designations	Description: protein RDM16-like Other designations: protein RDM16-like
Chromosome, Strand & Exon count	Chromosome: 6 Strand: minus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015443.3 Gene Start and end within genomic accession: 26773771 ...... 26786689
CDS Sequence	101258563
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Compound Repeat sequence: (GAT)3ggagattggagggataaggatgagg(AGAGA)2gaaaaggaggtttgag ggtaaagcaa(AGG)4
Repeat start & end within CDS	Repeat start: 322 Repeat end: 403
Forward primer	Primer sequence: CGAGGACGACAATGGTAGGG Tm(°C): 59.898 GC (%): 60 Size: 20
Reverse primer	Primer sequence: GCAATGGAACCAACAGCCTG Tm(°C): 60.038 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 290 End: 505 Product size (bp): 216
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101258563 NCBI Gene Symbol: LOC101258563
Gene Aliases
Gene description & Other designations	Description: protein RDM16-like Other designations: protein RDM16-like
Chromosome, Strand & Exon count	Chromosome: 6 Strand: minus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015443.3 Gene Start and end within genomic accession: 26773771 ...... 26786689
CDS Sequence	101258563
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Hexa Repeat sequence: (AGCAAA)2
Repeat start & end within CDS	Repeat start: 729 Repeat end: 740
Forward primer	Primer sequence: CAGGCTGTTGGTTCCATTGC Tm(°C): 60.038 GC (%): 55 Size: 20
Reverse primer	Primer sequence: TGGCCTTTTGAGTCCACTCC Tm(°C): 59.89 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 486 End: 780 Product size (bp): 295
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101258563 NCBI Gene Symbol: LOC101258563
Gene Aliases
Gene description & Other designations	Description: protein RDM16-like Other designations: protein RDM16-like
Chromosome, Strand & Exon count	Chromosome: 6 Strand: minus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015443.3 Gene Start and end within genomic accession: 26773771 ...... 26786689
CDS Sequence	101258563
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Hexa Repeat sequence: (AACAAG)2
Repeat start & end within CDS	Repeat start: 799 Repeat end: 810
Forward primer	Primer sequence: CTGGCAAGAGTGGGAGCTTA Tm(°C): 59.385 GC (%): 55 Size: 20
Reverse primer	Primer sequence: TTTTGAGCTGTGAGCCCCTT Tm(°C): 59.814 GC (%): 50 Size: 20
Primer start, end within sequence and product size	Start: 694 End: 1006 Product size (bp): 313
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101258563 NCBI Gene Symbol: LOC101258563
Gene Aliases
Gene description & Other designations	Description: protein RDM16-like Other designations: protein RDM16-like
Chromosome, Strand & Exon count	Chromosome: 6 Strand: minus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015443.3 Gene Start and end within genomic accession: 26773771 ...... 26786689
CDS Sequence	101258563
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Penta Repeat sequence: (GTAAT)2
Repeat start & end within CDS	Repeat start: 1637 Repeat end: 1646
Forward primer	Primer sequence: CAAGCACAGTTGACCAAGGC Tm(°C): 59.969 GC (%): 55 Size: 20
Reverse primer	Primer sequence: TGGCCAATCTTCGCTGAGTT Tm(°C): 59.963 GC (%): 50 Size: 20
Primer start, end within sequence and product size	Start: 1470 End: 1797 Product size (bp): 328
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101258563 NCBI Gene Symbol: LOC101258563
Gene Aliases
Gene description & Other designations	Description: protein RDM16-like Other designations: protein RDM16-like
Chromosome, Strand & Exon count	Chromosome: 6 Strand: minus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015443.3 Gene Start and end within genomic accession: 26773771 ...... 26786689
CDS Sequence	101258563
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Tri Repeat sequence: (TGT)4
Repeat start & end within CDS	Repeat start: 2178 Repeat end: 2189
Forward primer	Primer sequence: TGACTGGGTGTGCAGTGATC Tm(°C): 59.965 GC (%): 55 Size: 20
Reverse primer	Primer sequence: TGCCAGACCAACACACACTT Tm(°C): 60.035 GC (%): 50 Size: 20
Primer start, end within sequence and product size	Start: 2143 End: 2338 Product size (bp): 196
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101258563 NCBI Gene Symbol: LOC101258563
Gene Aliases
Gene description & Other designations	Description: protein RDM16-like Other designations: protein RDM16-like
Chromosome, Strand & Exon count	Chromosome: 6 Strand: minus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015443.3 Gene Start and end within genomic accession: 26773771 ...... 26786689
CDS Sequence	101258563
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Compound Repeat sequence: (TGAAGG)3(TGAAGA)2taagcctctcaacaa(GT)4
Repeat start & end within CDS	Repeat start: 2277 Repeat end: 2329
Forward primer	Primer sequence: TGACTGGGTGTGCAGTGATC Tm(°C): 59.965 GC (%): 55 Size: 20
Reverse primer	Primer sequence: AACTTTACGAGCAGCAGCCT Tm(°C): 59.965 GC (%): 50 Size: 20
Primer start, end within sequence and product size	Start: 2143 End: 2414 Product size (bp): 272
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101258563 NCBI Gene Symbol: LOC101258563
Gene Aliases
Gene description & Other designations	Description: protein RDM16-like Other designations: protein RDM16-like
Chromosome, Strand & Exon count	Chromosome: 6 Strand: minus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015443.3 Gene Start and end within genomic accession: 26773771 ...... 26786689
CDS Sequence	101258563
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Tri Repeat sequence: (GCT)3
Repeat start & end within CDS	Repeat start: 2398 Repeat end: 2406
Forward primer	Primer sequence: AGTGTGTGTTGGTCTGGCAA Tm(°C): 60.035 GC (%): 50 Size: 20
Reverse primer	Primer sequence: ACCCCAGCATCAGCAAAAAC Tm(°C): 59.319 GC (%): 50 Size: 20
Primer start, end within sequence and product size	Start: 2320 End: 2431 Product size (bp): 112
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Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101258563 NCBI Gene Symbol: LOC101258563
Gene Aliases
Gene description & Other designations	Description: protein RDM16-like Other designations: protein RDM16-like
Chromosome, Strand & Exon count	Chromosome: 6 Strand: minus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015443.3 Gene Start and end within genomic accession: 26773771 ...... 26786689
CDS Sequence	101258563
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Hexa Repeat sequence: (GAGAAA)2
Repeat start & end within CDS	Repeat start: 7 Repeat end: 18
Forward primer	Primer sequence: None Tm(°C): GC (%): Size:
Reverse primer	Primer sequence: None Tm(°C): GC (%): Size:
Primer start, end within sequence and product size	Start: End: Product size (bp):
JBrowse View	JBrowse

Enzyme Id: K12843
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 101258563 NCBI Gene Symbol: LOC101258563
Gene Aliases
Gene description & Other designations	Description: protein RDM16-like Other designations: protein RDM16-like
Chromosome, Strand & Exon count	Chromosome: 6 Strand: minus Exon count: 6
Gene Location within genomic sequence	Genomic accession No. NC_015443.3 Gene Start and end within genomic accession: 26773771 ...... 26786689
CDS Sequence	101258563
	Marker Information
Marker Type	PGTM
Repeat type & sequence	Repeat type: Repeat sequence:
Repeat start & end within CDS	Repeat start: Repeat end:
Forward primer	Primer sequence: CAAGCACAGTTGACCAAGGC Tm(°C): 59.969 GC (%): 55 Size: 20
Reverse primer	Primer sequence: CATCCCACCACTCAACGTCA Tm(°C): 59.965 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 1470 End: 1593 Product size (bp): 124
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