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Statistics

Number of enzymes: 1
Total Number of designed primers: 13
Number of PGTM primers:     1
Number of PMTM primers:     12

Enzyme Id:  K12822
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253282     NCBI Gene Symbol: LOC101253282
Gene Aliases
Gene description & Other designations Description:   RNA-binding protein 25      Other designations:   RNA-binding protein 25
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_015442.3      Gene Start and end within genomic accession: 433930 ...... 439581
CDS Sequence 101253282
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (TGGTGT)2agctcctatgttctcgcctctt(CCTAAC)2tt(GCA)3
Repeat start & end within CDS Repeat start: 204     Repeat end: 260
Forward primer Primer sequence:   TTACTCCGGTACACCTCCGT     Tm(°C): 59.962     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCACAGAATTCACTCCGGGA     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 149     End: 312     Product size (bp): 164
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Enzyme Id:  K12822
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253282     NCBI Gene Symbol: LOC101253282
Gene Aliases
Gene description & Other designations Description:   RNA-binding protein 25      Other designations:   RNA-binding protein 25
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_015442.3      Gene Start and end within genomic accession: 433930 ...... 439581
CDS Sequence 101253282
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TATGC)2
Repeat start & end within CDS Repeat start: 391     Repeat end: 400
Forward primer Primer sequence:   TCCCGGAGTGAATTCTGTGC     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGCCATCTGTGTGTACGGTG     Tm(°C): 60.037     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 293     End: 527     Product size (bp): 235
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Enzyme Id:  K12822
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253282     NCBI Gene Symbol: LOC101253282
Gene Aliases
Gene description & Other designations Description:   RNA-binding protein 25      Other designations:   RNA-binding protein 25
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_015442.3      Gene Start and end within genomic accession: 433930 ...... 439581
CDS Sequence 101253282
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GCCAG)2
Repeat start & end within CDS Repeat start: 631     Repeat end: 640
Forward primer Primer sequence:   CACCGTACACACAGATGGCT     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TTAATGCACGGAGAACCCCC     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 508     End: 855     Product size (bp): 348
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Enzyme Id:  K12822
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253282     NCBI Gene Symbol: LOC101253282
Gene Aliases
Gene description & Other designations Description:   RNA-binding protein 25      Other designations:   RNA-binding protein 25
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_015442.3      Gene Start and end within genomic accession: 433930 ...... 439581
CDS Sequence 101253282
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TGGTC)2
Repeat start & end within CDS Repeat start: 1140     Repeat end: 1149
Forward primer Primer sequence:   GGGGGTTCTCCGTGCATTAA     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCAAGGCCTCTTTGTCAGCT     Tm(°C): 60.251     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 836     End: 1185     Product size (bp): 350
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Enzyme Id:  K12822
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253282     NCBI Gene Symbol: LOC101253282
Gene Aliases
Gene description & Other designations Description:   RNA-binding protein 25      Other designations:   RNA-binding protein 25
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_015442.3      Gene Start and end within genomic accession: 433930 ...... 439581
CDS Sequence 101253282
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (CCACCT)3
Repeat start & end within CDS Repeat start: 1234     Repeat end: 1251
Forward primer Primer sequence:   GCTGACAAAGAGGCCTTGGA     Tm(°C): 60.251     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GTCCGGTGAGCTAATCTCGG     Tm(°C): 59.969     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 1167     End: 1409     Product size (bp): 243
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Enzyme Id:  K12822
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253282     NCBI Gene Symbol: LOC101253282
Gene Aliases
Gene description & Other designations Description:   RNA-binding protein 25      Other designations:   RNA-binding protein 25
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_015442.3      Gene Start and end within genomic accession: 433930 ...... 439581
CDS Sequence 101253282
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (ATG)3
Repeat start & end within CDS Repeat start: 1346     Repeat end: 1354
Forward primer Primer sequence:   GCTGACAAAGAGGCCTTGGA     Tm(°C): 60.251     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GTCCGGTGAGCTAATCTCGG     Tm(°C): 59.969     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 1167     End: 1409     Product size (bp): 243
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Enzyme Id:  K12822
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253282     NCBI Gene Symbol: LOC101253282
Gene Aliases
Gene description & Other designations Description:   RNA-binding protein 25      Other designations:   RNA-binding protein 25
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_015442.3      Gene Start and end within genomic accession: 433930 ...... 439581
CDS Sequence 101253282
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (AAAGG)2gaactcgaaaggtat(GA)4acgtgaacaagaacgagcca(AG)4aa
g(GA)4
Repeat start & end within CDS Repeat start: 1464     Repeat end: 1535
Forward primer Primer sequence:   CGAGATTAGCTCACCGGACC     Tm(°C): 59.969     GC (%): 60     Size: 20
Reverse primer Primer sequence:   CATTTCCGTTCCTGAGCCCT     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1391     End: 1666     Product size (bp): 276
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Enzyme Id:  K12822
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253282     NCBI Gene Symbol: LOC101253282
Gene Aliases
Gene description & Other designations Description:   RNA-binding protein 25      Other designations:   RNA-binding protein 25
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_015442.3      Gene Start and end within genomic accession: 433930 ...... 439581
CDS Sequence 101253282
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (AG)5
Repeat start & end within CDS Repeat start: 1624     Repeat end: 1633
Forward primer Primer sequence:   CGAGATTAGCTCACCGGACC     Tm(°C): 59.969     GC (%): 60     Size: 20
Reverse primer Primer sequence:   CATTTCCGTTCCTGAGCCCT     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1391     End: 1666     Product size (bp): 276
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Enzyme Id:  K12822
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253282     NCBI Gene Symbol: LOC101253282
Gene Aliases
Gene description & Other designations Description:   RNA-binding protein 25      Other designations:   RNA-binding protein 25
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_015442.3      Gene Start and end within genomic accession: 433930 ...... 439581
CDS Sequence 101253282
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GAAAA)2cacaggactagtgac(GAGGA)2aaagacggc(AG)4aaggaagatg
acttggctgacaggttta(AAG)4agattgctgaggct(AAGAG)2cagacgagg
aacatcagaaaaaagaac(AGG)3
Repeat start & end within CDS Repeat start: 1710     Repeat end: 1861
Forward primer Primer sequence:   AGGGCTCAGGAACGGAAATG     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAACTCCTTCACCCTGGCTT     Tm(°C): 59.889     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1647     End: 1992     Product size (bp): 346
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Enzyme Id:  K12822
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253282     NCBI Gene Symbol: LOC101253282
Gene Aliases
Gene description & Other designations Description:   RNA-binding protein 25      Other designations:   RNA-binding protein 25
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_015442.3      Gene Start and end within genomic accession: 433930 ...... 439581
CDS Sequence 101253282
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TTTAA)2
Repeat start & end within CDS Repeat start: 1966     Repeat end: 1975
Forward primer Primer sequence:   AGGGCTCAGGAACGGAAATG     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGTGCAACTCCTTCACCCTG     Tm(°C): 60.179     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1647     End: 1996     Product size (bp): 350
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Enzyme Id:  K12822
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253282     NCBI Gene Symbol: LOC101253282
Gene Aliases
Gene description & Other designations Description:   RNA-binding protein 25      Other designations:   RNA-binding protein 25
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_015442.3      Gene Start and end within genomic accession: 433930 ...... 439581
CDS Sequence 101253282
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GGATGA)2ggatactca(AAAAG)2
Repeat start & end within CDS Repeat start: 2139     Repeat end: 2169
Forward primer Primer sequence:   AGAATCCGTGCGTGCAGTTA     Tm(°C): 60.038     GC (%): 50     Size: 20
Reverse primer Primer sequence:   ATCCCTCTGGCTGGATCTGT     Tm(°C): 60.03     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 2012     End: 2360     Product size (bp): 349
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Enzyme Id:  K12822
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253282     NCBI Gene Symbol: LOC101253282
Gene Aliases
Gene description & Other designations Description:   RNA-binding protein 25      Other designations:   RNA-binding protein 25
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_015442.3      Gene Start and end within genomic accession: 433930 ...... 439581
CDS Sequence 101253282
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAG)3
Repeat start & end within CDS Repeat start: 2626     Repeat end: 2634
Forward primer Primer sequence:   CGAACCAGGGATGACGGTAG     Tm(°C): 59.898     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TCTCCAGCATCTCAGTTGCC     Tm(°C): 59.749     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 2388     End: 2700     Product size (bp): 313
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Enzyme Id:  K12822
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253282     NCBI Gene Symbol: LOC101253282
Gene Aliases
Gene description & Other designations Description:   RNA-binding protein 25      Other designations:   RNA-binding protein 25
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_015442.3      Gene Start and end within genomic accession: 433930 ...... 439581
CDS Sequence 101253282
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GATGAAGAATCCGTGCGTGC     Tm(°C): 59.973     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ATCCCTCTGGCTGGATCTGT     Tm(°C): 60.03     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 2007     End: 2360     Product size (bp): 354
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