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Statistics

Number of enzymes: 1
Total Number of designed primers: 15
Number of PGTM primers:     2
Number of PMTM primers:     13

Enzyme Id:  K12821
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101256011     NCBI Gene Symbol: LOC101256011
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 40A      Other designations:   pre-mRNA-processing protein 40A
Chromosome, Strand & Exon count Chromosome:   7     Strand:   minus     Exon count:   29
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 21367272 ...... 21400197
CDS Sequence 101256011
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GGTCAA)2
Repeat start & end within CDS Repeat start: 205     Repeat end: 216
Forward primer Primer sequence:   ACAGCAAGGCCAGCATTTTG     Tm(°C): 59.966     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TGACGGTGTTCCATGACCTG     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 116     End: 287     Product size (bp): 172
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Enzyme Id:  K12821
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101256011     NCBI Gene Symbol: LOC101256011
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 40A      Other designations:   pre-mRNA-processing protein 40A
Chromosome, Strand & Exon count Chromosome:   7     Strand:   minus     Exon count:   29
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 21367272 ...... 21400197
CDS Sequence 101256011
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TCA)3
Repeat start & end within CDS Repeat start: 400     Repeat end: 408
Forward primer Primer sequence:   GAACACCGTCATCTCAGGCA     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCTGCTGAGAAGCTGTGCTC     Tm(°C): 60.038     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 277     End: 579     Product size (bp): 303
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Enzyme Id:  K12821
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101256011     NCBI Gene Symbol: LOC101256011
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 40A      Other designations:   pre-mRNA-processing protein 40A
Chromosome, Strand & Exon count Chromosome:   7     Strand:   minus     Exon count:   29
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 21367272 ...... 21400197
CDS Sequence 101256011
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (AG)4
Repeat start & end within CDS Repeat start: 690     Repeat end: 697
Forward primer Primer sequence:   CGTTGGAGCTGATGACACCT     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AACAGCAGGCGTAACTGGAA     Tm(°C): 59.892     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 667     End: 995     Product size (bp): 329
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Enzyme Id:  K12821
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101256011     NCBI Gene Symbol: LOC101256011
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 40A      Other designations:   pre-mRNA-processing protein 40A
Chromosome, Strand & Exon count Chromosome:   7     Strand:   minus     Exon count:   29
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 21367272 ...... 21400197
CDS Sequence 101256011
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (AAAGGA)2
Repeat start & end within CDS Repeat start: 2307     Repeat end: 2318
Forward primer Primer sequence:   TGGCTTATCAGGCTGTTGCA     Tm(°C): 59.962     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TGCAAGCCTTTGACGCTTTT     Tm(°C): 59.541     GC (%): 45     Size: 20
Primer start, end within sequence and product size Start: 2050     End: 2351     Product size (bp): 302
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Enzyme Id:  K12821
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101256011     NCBI Gene Symbol: LOC101256011
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 40A      Other designations:   pre-mRNA-processing protein 40A
Chromosome, Strand & Exon count Chromosome:   7     Strand:   minus     Exon count:   29
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 21367272 ...... 21400197
CDS Sequence 101256011
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AAAAG)2
Repeat start & end within CDS Repeat start: 2520     Repeat end: 2529
Forward primer Primer sequence:   CTCAAGGGCAGTCTTTGAGGA     Tm(°C): 59.651     GC (%): 52.381     Size: 21
Reverse primer Primer sequence:   ATGGTGTCGCTTCCGATGTT     Tm(°C): 60.036     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 2471     End: 2771     Product size (bp): 301
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Enzyme Id:  K12821
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101256011     NCBI Gene Symbol: LOC101256011
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 40A      Other designations:   pre-mRNA-processing protein 40A
Chromosome, Strand & Exon count Chromosome:   7     Strand:   minus     Exon count:   29
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 21367272 ...... 21400197
CDS Sequence 101256011
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AAGAG)2
Repeat start & end within CDS Repeat start: 2582     Repeat end: 2591
Forward primer Primer sequence:   CGCAAACGTGAGGAGGAAAA     Tm(°C): 59.058     GC (%): 50     Size: 20
Reverse primer Primer sequence:   ATGGTGTCGCTTCCGATGTT     Tm(°C): 60.036     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 2532     End: 2771     Product size (bp): 240
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Enzyme Id:  K12821
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101256011     NCBI Gene Symbol: LOC101256011
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 40A      Other designations:   pre-mRNA-processing protein 40A
Chromosome, Strand & Exon count Chromosome:   7     Strand:   minus     Exon count:   29
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 21367272 ...... 21400197
CDS Sequence 101256011
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (GA)4
Repeat start & end within CDS Repeat start: 2893     Repeat end: 2900
Forward primer Primer sequence:   AACATCGGAAGCGACACCAT     Tm(°C): 60.036     GC (%): 50     Size: 20
Reverse primer Primer sequence:   AGCTCTTCATGCCCACCATT     Tm(°C): 59.666     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 2752     End: 3046     Product size (bp): 295
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Enzyme Id:  K12821
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101256011     NCBI Gene Symbol: LOC101256011
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 40A      Other designations:   pre-mRNA-processing protein 40A
Chromosome, Strand & Exon count Chromosome:   7     Strand:   minus     Exon count:   29
Gene Location within genomic sequence Genomic accession No. NC_015444.3      Gene Start and end within genomic accession: 21367272 ...... 21400197
CDS Sequence 101256011
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   ACAGCAAGGCCAGCATTTTG     Tm(°C): 59.966     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TGACGGTGTTCCATGACCTG     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 116     End: 287     Product size (bp): 172
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Enzyme Id:  K12821
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101267083     NCBI Gene Symbol: LOC101267083
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 40A      Other designations:   pre-mRNA-processing protein 40A
Chromosome, Strand & Exon count Chromosome:   11     Strand:   plus     Exon count:   29
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 33151776 ...... 33172874
CDS Sequence 101267083
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GATTG)2
Repeat start & end within CDS Repeat start: 580     Repeat end: 589
Forward primer Primer sequence:   GAACTAGCAGCTCCGTTGGT     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AATCAGAGGCGTGCGAATCA     Tm(°C): 60.108     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 510     End: 834     Product size (bp): 325
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Enzyme Id:  K12821
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101267083     NCBI Gene Symbol: LOC101267083
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 40A      Other designations:   pre-mRNA-processing protein 40A
Chromosome, Strand & Exon count Chromosome:   11     Strand:   plus     Exon count:   29
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 33151776 ...... 33172874
CDS Sequence 101267083
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAG)3
Repeat start & end within CDS Repeat start: 1855     Repeat end: 1863
Forward primer Primer sequence:   AGCTGATGAAAGATGCCCCC     Tm(°C): 60.106     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGCTCATCCACGACATCTGC     Tm(°C): 60.179     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1772     End: 2086     Product size (bp): 315
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Enzyme Id:  K12821
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101267083     NCBI Gene Symbol: LOC101267083
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 40A      Other designations:   pre-mRNA-processing protein 40A
Chromosome, Strand & Exon count Chromosome:   11     Strand:   plus     Exon count:   29
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 33151776 ...... 33172874
CDS Sequence 101267083
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (ATCAAA)2
Repeat start & end within CDS Repeat start: 1993     Repeat end: 2004
Forward primer Primer sequence:   GAGAGGGAAGAGGAGGAGCA     Tm(°C): 60.033     GC (%): 60     Size: 20
Reverse primer Primer sequence:   AATCGTCAAGCGTCCAGGTT     Tm(°C): 59.965     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1845     End: 2178     Product size (bp): 334
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Enzyme Id:  K12821
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101267083     NCBI Gene Symbol: LOC101267083
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 40A      Other designations:   pre-mRNA-processing protein 40A
Chromosome, Strand & Exon count Chromosome:   11     Strand:   plus     Exon count:   29
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 33151776 ...... 33172874
CDS Sequence 101267083
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (GA)4
Repeat start & end within CDS Repeat start: 2272     Repeat end: 2279
Forward primer Primer sequence:   CTGACATCAACCTGGACGCT     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCCGGTCCTTCTCTTTTGCC     Tm(°C): 59.964     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 2151     End: 2484     Product size (bp): 334
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Enzyme Id:  K12821
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101267083     NCBI Gene Symbol: LOC101267083
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 40A      Other designations:   pre-mRNA-processing protein 40A
Chromosome, Strand & Exon count Chromosome:   11     Strand:   plus     Exon count:   29
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 33151776 ...... 33172874
CDS Sequence 101267083
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (AAAAG)2gcaaaagagaaggaccggaagcgtcaagaggataaggcaagaaaaga
g(AAGGA)2aagatagagaa(AAG)3gaaaaacatagaagggataaacacagagg
ggataagagcaggaa(AG)4
Repeat start & end within CDS Repeat start: 2457     Repeat end: 2596
Forward primer Primer sequence:   CGTCAAAATGGGAGGACTGC     Tm(°C): 59.195     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCTCCTTGTCACTGTCTCTCT     Tm(°C): 57.483     GC (%): 47.619     Size: 21
Primer start, end within sequence and product size Start: 2359     End: 2682     Product size (bp): 324
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Enzyme Id:  K12821
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101267083     NCBI Gene Symbol: LOC101267083
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 40A      Other designations:   pre-mRNA-processing protein 40A
Chromosome, Strand & Exon count Chromosome:   11     Strand:   plus     Exon count:   29
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 33151776 ...... 33172874
CDS Sequence 101267083
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GATGAC)2
Repeat start & end within CDS Repeat start: 2917     Repeat end: 2928
Forward primer Primer sequence:   CGCAAGCGGCATAGACATTC     Tm(°C): 60.042     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACTCCGTCTGTGTAGGTCCA     Tm(°C): 59.888     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 2892     End: 3011     Product size (bp): 120
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Enzyme Id:  K12821
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101267083     NCBI Gene Symbol: LOC101267083
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 40A      Other designations:   pre-mRNA-processing protein 40A
Chromosome, Strand & Exon count Chromosome:   11     Strand:   plus     Exon count:   29
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 33151776 ...... 33172874
CDS Sequence 101267083
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GAGAGGGAAGAGGAGGAGCA     Tm(°C): 60.033     GC (%): 60     Size: 20
Reverse primer Primer sequence:   AATCGTCAAGCGTCCAGGTT     Tm(°C): 59.965     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1845     End: 2178     Product size (bp): 334
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