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Statistics

Number of enzymes: 1
Total Number of designed primers: 7
Number of PGTM primers:     1
Number of PMTM primers:     6

Enzyme Id:  K12820
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101263245     NCBI Gene Symbol: LOC101263245
Gene Aliases
Gene description & Other designations Description:   probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2      Other designations:   probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2
Chromosome, Strand & Exon count Chromosome:   1     Strand:   minus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_015438.3      Gene Start and end within genomic accession: 78200688 ...... 78206499
CDS Sequence 101263245
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AGACC)2
Repeat start & end within CDS Repeat start: 622     Repeat end: 631
Forward primer Primer sequence:   CTGTCTCTCGTCGTGTTGCT     Tm(°C): 60.04     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGCTTCAAGTGTGGCACTCA     Tm(°C): 60.107     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 373     End: 665     Product size (bp): 293
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Enzyme Id:  K12820
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101263245     NCBI Gene Symbol: LOC101263245
Gene Aliases
Gene description & Other designations Description:   probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2      Other designations:   probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2
Chromosome, Strand & Exon count Chromosome:   1     Strand:   minus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_015438.3      Gene Start and end within genomic accession: 78200688 ...... 78206499
CDS Sequence 101263245
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (GA)4
Repeat start & end within CDS Repeat start: 757     Repeat end: 764
Forward primer Primer sequence:   TGAGTGCCACACTTGAAGCA     Tm(°C): 60.107     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TCTCCTCTTCTCCGGTGAGG     Tm(°C): 60.034     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 646     End: 855     Product size (bp): 210
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Enzyme Id:  K12820
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101263245     NCBI Gene Symbol: LOC101263245
Gene Aliases
Gene description & Other designations Description:   probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2      Other designations:   probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2
Chromosome, Strand & Exon count Chromosome:   1     Strand:   minus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_015438.3      Gene Start and end within genomic accession: 78200688 ...... 78206499
CDS Sequence 101263245
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CCA)3
Repeat start & end within CDS Repeat start: 985     Repeat end: 993
Forward primer Primer sequence:   GTAGGCCCTGTTAAAGCGGT     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GTTCTTCCAGCACGTCCTGA     Tm(°C): 59.966     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 909     End: 1201     Product size (bp): 293
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Enzyme Id:  K12820
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101263245     NCBI Gene Symbol: LOC101263245
Gene Aliases
Gene description & Other designations Description:   probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2      Other designations:   probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2
Chromosome, Strand & Exon count Chromosome:   1     Strand:   minus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_015438.3      Gene Start and end within genomic accession: 78200688 ...... 78206499
CDS Sequence 101263245
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAT)3
Repeat start & end within CDS Repeat start: 1423     Repeat end: 1431
Forward primer Primer sequence:   GGACGTGCTGGAAGAACTCA     Tm(°C): 59.966     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCATCTGAGGGTCGAGAGGA     Tm(°C): 60.106     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 1185     End: 1488     Product size (bp): 304
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Enzyme Id:  K12820
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101263245     NCBI Gene Symbol: LOC101263245
Gene Aliases
Gene description & Other designations Description:   probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2      Other designations:   probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2
Chromosome, Strand & Exon count Chromosome:   1     Strand:   minus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_015438.3      Gene Start and end within genomic accession: 78200688 ...... 78206499
CDS Sequence 101263245
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TCGGT)2
Repeat start & end within CDS Repeat start: 1620     Repeat end: 1629
Forward primer Primer sequence:   TCCTCTCGACCCTCAGATGG     Tm(°C): 60.106     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TGAACCTGGCCATTATGCGA     Tm(°C): 59.746     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1469     End: 1788     Product size (bp): 320
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Enzyme Id:  K12820
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101263245     NCBI Gene Symbol: LOC101263245
Gene Aliases
Gene description & Other designations Description:   probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2      Other designations:   probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2
Chromosome, Strand & Exon count Chromosome:   1     Strand:   minus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_015438.3      Gene Start and end within genomic accession: 78200688 ...... 78206499
CDS Sequence 101263245
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (TA)4
Repeat start & end within CDS Repeat start: 1977     Repeat end: 1984
Forward primer Primer sequence:   ATTGCCTGGATCACAAGCCA     Tm(°C): 59.96     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TAACCGCTCAAGCACTCGTT     Tm(°C): 59.967     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1948     End: 2123     Product size (bp): 176
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Enzyme Id:  K12820
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101263245     NCBI Gene Symbol: LOC101263245
Gene Aliases
Gene description & Other designations Description:   probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2      Other designations:   probable pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH2
Chromosome, Strand & Exon count Chromosome:   1     Strand:   minus     Exon count:   7
Gene Location within genomic sequence Genomic accession No. NC_015438.3      Gene Start and end within genomic accession: 78200688 ...... 78206499
CDS Sequence 101263245
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GCATGACGGCTTCGATCAAC     Tm(°C): 59.973     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGCAACACGACGAGAGACAG     Tm(°C): 60.04     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 94     End: 392     Product size (bp): 299
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