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Statistics

Number of enzymes: 1
Total Number of designed primers: 16
Number of PGTM primers:     2
Number of PMTM primers:     14

Enzyme Id:  K12813
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253458     NCBI Gene Symbol: LOC101253458
Gene Aliases DHX16
Gene description & Other designations Description:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1      Other designations:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1|DEAH (Asp-Glu-Ala-His) box polypeptide 16|putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Chromosome, Strand & Exon count Chromosome:   12     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_015449.3      Gene Start and end within genomic accession: 7217951 ...... 7247171
CDS Sequence 101253458
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (GAAGAC)2
Repeat start & end within CDS Repeat start: 52     Repeat end: 63
Forward primer Primer sequence:   AAAGAGGAATACGGCCGGTG     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCTCCCAAGCTTGTTGTGGA     Tm(°C): 60.179     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 32     End: 342     Product size (bp): 311
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Enzyme Id:  K12813
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253458     NCBI Gene Symbol: LOC101253458
Gene Aliases DHX16
Gene description & Other designations Description:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1      Other designations:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1|DEAH (Asp-Glu-Ala-His) box polypeptide 16|putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Chromosome, Strand & Exon count Chromosome:   12     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_015449.3      Gene Start and end within genomic accession: 7217951 ...... 7247171
CDS Sequence 101253458
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (AGA)3
Repeat start & end within CDS Repeat start: 149     Repeat end: 157
Forward primer Primer sequence:   CCGGTGAAGACGAAGACGAA     Tm(°C): 60.04     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCTCCCAAGCTTGTTGTGGA     Tm(°C): 60.179     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 46     End: 342     Product size (bp): 297
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Enzyme Id:  K12813
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253458     NCBI Gene Symbol: LOC101253458
Gene Aliases DHX16
Gene description & Other designations Description:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1      Other designations:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1|DEAH (Asp-Glu-Ala-His) box polypeptide 16|putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Chromosome, Strand & Exon count Chromosome:   12     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_015449.3      Gene Start and end within genomic accession: 7217951 ...... 7247171
CDS Sequence 101253458
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AAATC)2
Repeat start & end within CDS Repeat start: 345     Repeat end: 354
Forward primer Primer sequence:   CCACAACAAGCTTGGGAGGA     Tm(°C): 60.179     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TTGAGTTGTCTTCCCGGAGC     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 324     End: 632     Product size (bp): 309
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Enzyme Id:  K12813
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253458     NCBI Gene Symbol: LOC101253458
Gene Aliases DHX16
Gene description & Other designations Description:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1      Other designations:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1|DEAH (Asp-Glu-Ala-His) box polypeptide 16|putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Chromosome, Strand & Exon count Chromosome:   12     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_015449.3      Gene Start and end within genomic accession: 7217951 ...... 7247171
CDS Sequence 101253458
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (ATCAA)2ctataatt(GATTCA)2(GATGTT)2
Repeat start & end within CDS Repeat start: 436     Repeat end: 477
Forward primer Primer sequence:   CCACAACAAGCTTGGGAGGA     Tm(°C): 60.179     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TTGAGTTGTCTTCCCGGAGC     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 324     End: 632     Product size (bp): 309
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Enzyme Id:  K12813
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253458     NCBI Gene Symbol: LOC101253458
Gene Aliases DHX16
Gene description & Other designations Description:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1      Other designations:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1|DEAH (Asp-Glu-Ala-His) box polypeptide 16|putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Chromosome, Strand & Exon count Chromosome:   12     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_015449.3      Gene Start and end within genomic accession: 7217951 ...... 7247171
CDS Sequence 101253458
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TATTC)2
Repeat start & end within CDS Repeat start: 778     Repeat end: 787
Forward primer Primer sequence:   CTGCGCGTGTTTCACATGAA     Tm(°C): 60.041     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CATCAAGGGTGGCGCTAGAT     Tm(°C): 59.893     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 727     End: 1011     Product size (bp): 285
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Enzyme Id:  K12813
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253458     NCBI Gene Symbol: LOC101253458
Gene Aliases DHX16
Gene description & Other designations Description:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1      Other designations:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1|DEAH (Asp-Glu-Ala-His) box polypeptide 16|putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Chromosome, Strand & Exon count Chromosome:   12     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_015449.3      Gene Start and end within genomic accession: 7217951 ...... 7247171
CDS Sequence 101253458
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (ACTTC)2agattcatgcaactcaaccacag(GGTGAT)2
Repeat start & end within CDS Repeat start: 1135     Repeat end: 1179
Forward primer Primer sequence:   ATCTAGCGCCACCCTTGATG     Tm(°C): 59.893     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CTCCTGCCCCGTCAAAAAGA     Tm(°C): 60.251     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 992     End: 1205     Product size (bp): 214
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Enzyme Id:  K12813
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253458     NCBI Gene Symbol: LOC101253458
Gene Aliases DHX16
Gene description & Other designations Description:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1      Other designations:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1|DEAH (Asp-Glu-Ala-His) box polypeptide 16|putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Chromosome, Strand & Exon count Chromosome:   12     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_015449.3      Gene Start and end within genomic accession: 7217951 ...... 7247171
CDS Sequence 101253458
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TTGCT)2
Repeat start & end within CDS Repeat start: 1988     Repeat end: 1997
Forward primer Primer sequence:   CTCTGGATCCGATGCTGTCC     Tm(°C): 59.967     GC (%): 60     Size: 20
Reverse primer Primer sequence:   CCCTTTCAAGCCGACCTTCT     Tm(°C): 59.963     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1804     End: 2124     Product size (bp): 321
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Enzyme Id:  K12813
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101253458     NCBI Gene Symbol: LOC101253458
Gene Aliases DHX16
Gene description & Other designations Description:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1      Other designations:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1|DEAH (Asp-Glu-Ala-His) box polypeptide 16|putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Chromosome, Strand & Exon count Chromosome:   12     Strand:   minus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_015449.3      Gene Start and end within genomic accession: 7217951 ...... 7247171
CDS Sequence 101253458
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GTGGCTCCTATCTCGAAGGC     Tm(°C): 59.967     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GACAGCATCGGATCCAGAGG     Tm(°C): 59.967     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 1479     End: 1822     Product size (bp): 344
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Enzyme Id:  K12813
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265017     NCBI Gene Symbol: LOC101265017
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1-like      Other designations:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1-like
Chromosome, Strand & Exon count Chromosome:   3     Strand:   minus     Exon count:   28
Gene Location within genomic sequence Genomic accession No. NC_015440.3      Gene Start and end within genomic accession: 66592694 ...... 66610538
CDS Sequence 101265017
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (TGA)3agatgacaatattgtcggtgtggaaagta(GTTCC)2
Repeat start & end within CDS Repeat start: 300     Repeat end: 347
Forward primer Primer sequence:   AAGGGAGGCTGCTATGTTGG     Tm(°C): 59.743     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AACTTTCTAGTCCCGGCAGC     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 245     End: 592     Product size (bp): 348
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Enzyme Id:  K12813
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265017     NCBI Gene Symbol: LOC101265017
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1-like      Other designations:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1-like
Chromosome, Strand & Exon count Chromosome:   3     Strand:   minus     Exon count:   28
Gene Location within genomic sequence Genomic accession No. NC_015440.3      Gene Start and end within genomic accession: 66592694 ...... 66610538
CDS Sequence 101265017
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GAA)3atacttcgggaccaa(AG)4
Repeat start & end within CDS Repeat start: 508     Repeat end: 539
Forward primer Primer sequence:   GGTTGAGACCCATGGAGACG     Tm(°C): 60.108     GC (%): 60     Size: 20
Reverse primer Primer sequence:   AACTTTCTAGTCCCGGCAGC     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 395     End: 592     Product size (bp): 198
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Enzyme Id:  K12813
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265017     NCBI Gene Symbol: LOC101265017
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1-like      Other designations:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1-like
Chromosome, Strand & Exon count Chromosome:   3     Strand:   minus     Exon count:   28
Gene Location within genomic sequence Genomic accession No. NC_015440.3      Gene Start and end within genomic accession: 66592694 ...... 66610538
CDS Sequence 101265017
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAA)3
Repeat start & end within CDS Repeat start: 622     Repeat end: 630
Forward primer Primer sequence:   GCTGCCGGGACTAGAAAGTT     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCTCACGTTCCTCTGCTTCT     Tm(°C): 60.038     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 573     End: 807     Product size (bp): 235
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Enzyme Id:  K12813
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265017     NCBI Gene Symbol: LOC101265017
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1-like      Other designations:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1-like
Chromosome, Strand & Exon count Chromosome:   3     Strand:   minus     Exon count:   28
Gene Location within genomic sequence Genomic accession No. NC_015440.3      Gene Start and end within genomic accession: 66592694 ...... 66610538
CDS Sequence 101265017
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GCTGA)2
Repeat start & end within CDS Repeat start: 1951     Repeat end: 1960
Forward primer Primer sequence:   ACTTGGACGCTGCAGTAGTC     Tm(°C): 60.038     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCGTCCGAGGGTTGTAAGAC     Tm(°C): 60.109     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 1798     End: 2145     Product size (bp): 348
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Enzyme Id:  K12813
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265017     NCBI Gene Symbol: LOC101265017
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1-like      Other designations:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1-like
Chromosome, Strand & Exon count Chromosome:   3     Strand:   minus     Exon count:   28
Gene Location within genomic sequence Genomic accession No. NC_015440.3      Gene Start and end within genomic accession: 66592694 ...... 66610538
CDS Sequence 101265017
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (CT)4
Repeat start & end within CDS Repeat start: 2326     Repeat end: 2333
Forward primer Primer sequence:   GTCTTACAACCCTCGGACGG     Tm(°C): 60.109     GC (%): 60     Size: 20
Reverse primer Primer sequence:   AGTCAACTCGCCAAGCTTGT     Tm(°C): 60.179     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 2126     End: 2459     Product size (bp): 334
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Enzyme Id:  K12813
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265017     NCBI Gene Symbol: LOC101265017
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1-like      Other designations:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1-like
Chromosome, Strand & Exon count Chromosome:   3     Strand:   minus     Exon count:   28
Gene Location within genomic sequence Genomic accession No. NC_015440.3      Gene Start and end within genomic accession: 66592694 ...... 66610538
CDS Sequence 101265017
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (ACAAGT)2
Repeat start & end within CDS Repeat start: 2528     Repeat end: 2539
Forward primer Primer sequence:   ACAAGCTTGGCGAGTTGACT     Tm(°C): 60.179     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CTCGGATGTCCCTTGCTCTC     Tm(°C): 59.896     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 2440     End: 2781     Product size (bp): 342
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Enzyme Id:  K12813
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265017     NCBI Gene Symbol: LOC101265017
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1-like      Other designations:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1-like
Chromosome, Strand & Exon count Chromosome:   3     Strand:   minus     Exon count:   28
Gene Location within genomic sequence Genomic accession No. NC_015440.3      Gene Start and end within genomic accession: 66592694 ...... 66610538
CDS Sequence 101265017
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AATTG)2
Repeat start & end within CDS Repeat start: 2814     Repeat end: 2823
Forward primer Primer sequence:   GAGAGCAAGGGACATCCGAG     Tm(°C): 59.896     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TTCAGTAACCTGCCGCATGT     Tm(°C): 59.964     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 2762     End: 3044     Product size (bp): 283
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Enzyme Id:  K12813
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101265017     NCBI Gene Symbol: LOC101265017
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1-like      Other designations:   pre-mRNA-splicing factor ATP-dependent RNA helicase DEAH1-like
Chromosome, Strand & Exon count Chromosome:   3     Strand:   minus     Exon count:   28
Gene Location within genomic sequence Genomic accession No. NC_015440.3      Gene Start and end within genomic accession: 66592694 ...... 66610538
CDS Sequence 101265017
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GCTGCCGGGACTAGAAAGTT     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GATCCCTGTATCGCTGCGAA     Tm(°C): 59.968     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 573     End: 960     Product size (bp): 388
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