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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     1
Number of PMTM primers:     2

Enzyme Id:  K12586
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322034     NCBI Gene Symbol: LOC103322034
Gene Aliases
Gene description & Other designations Description:   exosome complex component RRP43      Other designations:   exosome complex component RRP43
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 20473213 ...... 20477790
CDS Sequence 103322034
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TGGATG)2
Repeat start & end within CDS Repeat start: 41     Repeat end: 52
Forward primer Primer sequence:   GATGCTTCTGGGGATGTGCT     Tm(°C): 60.107     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAGGCCTAACAGTTGGGGAG     Tm(°C): 60.035     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 12     End: 327     Product size (bp): 316
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Enzyme Id:  K12586
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322034     NCBI Gene Symbol: LOC103322034
Gene Aliases
Gene description & Other designations Description:   exosome complex component RRP43      Other designations:   exosome complex component RRP43
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 20473213 ...... 20477790
CDS Sequence 103322034
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (ATG)3ggaa(AGT)3
Repeat start & end within CDS Repeat start: 554     Repeat end: 575
Forward primer Primer sequence:   CGGTTGCTGCCTTTTCTCAC     Tm(°C): 60.04     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGAAGCTCCCTCGCTCTTTG     Tm(°C): 60.037     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 508     End: 856     Product size (bp): 349
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Enzyme Id:  K12586
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103322034     NCBI Gene Symbol: LOC103322034
Gene Aliases
Gene description & Other designations Description:   exosome complex component RRP43      Other designations:   exosome complex component RRP43
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 20473213 ...... 20477790
CDS Sequence 103322034
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AGACGCCTGTTTCCTCTTCG     Tm(°C): 60.038     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAGGCCTAACAGTTGGGGAG     Tm(°C): 60.035     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 57     End: 327     Product size (bp): 271
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