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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     1
Number of PMTM primers:     2
Number of Failed designed PMTM primers: 1

Enzyme Id:  K08991

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   PHAVU_005G033000g     NCBI Gene Symbol: PHAVU_005G033000g
Gene Aliases PHAVU_005G033000g
Gene description & Other designations Description:   hypothetical protein      Other designations:   hypothetical protein
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   16
Gene Location within genomic sequence Genomic accession No. NC_023755.1      Gene Start and end within genomic accession: 3037611 ...... 3050454
CDS Sequence PHAVU_005G033000g
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TCAAG)2
Repeat start & end within CDS Repeat start: 186     Repeat end: 195
Forward primer Primer sequence:   GTCTGTAGCTCCCAAACCCC     Tm(°C): 60.035     GC (%): 60     Size: 20
Reverse primer Primer sequence:   CCTTTCCCTTTTTCTGGCGC     Tm(°C): 60.038     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 141     End: 457     Product size (bp): 317
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Enzyme Id:  K08991

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   PHAVU_005G033000g     NCBI Gene Symbol: PHAVU_005G033000g
Gene Aliases PHAVU_005G033000g
Gene description & Other designations Description:   hypothetical protein      Other designations:   hypothetical protein
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   16
Gene Location within genomic sequence Genomic accession No. NC_023755.1      Gene Start and end within genomic accession: 3037611 ...... 3050454
CDS Sequence PHAVU_005G033000g
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GGAAA)2
Repeat start & end within CDS Repeat start: 452     Repeat end: 461
Forward primer Primer sequence:   TGCCAATTGCGCCAGAAAAA     Tm(°C): 59.894     GC (%): 45     Size: 20
Reverse primer Primer sequence:   AGGCTTCTTTTGCTGGGTCA     Tm(°C): 59.814     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 430     End: 758     Product size (bp): 329
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Enzyme Id:  K08991

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   PHAVU_005G033000g     NCBI Gene Symbol: PHAVU_005G033000g
Gene Aliases PHAVU_005G033000g
Gene description & Other designations Description:   hypothetical protein      Other designations:   hypothetical protein
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   16
Gene Location within genomic sequence Genomic accession No. NC_023755.1      Gene Start and end within genomic accession: 3037611 ...... 3050454
CDS Sequence PHAVU_005G033000g
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GGTGC)2
Repeat start & end within CDS Repeat start: 18     Repeat end: 27
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K08991

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   PHAVU_005G033000g     NCBI Gene Symbol: PHAVU_005G033000g
Gene Aliases PHAVU_005G033000g
Gene description & Other designations Description:   hypothetical protein      Other designations:   hypothetical protein
Chromosome, Strand & Exon count Chromosome:   5     Strand:   minus     Exon count:   16
Gene Location within genomic sequence Genomic accession No. NC_023755.1      Gene Start and end within genomic accession: 3037611 ...... 3050454
CDS Sequence PHAVU_005G033000g
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TTCATCTGACCACCCCATGC     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCCAACTGGCAATCGTCGAA     Tm(°C): 59.966     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1055     End: 1289     Product size (bp): 235
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