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Statistics

Number of enzymes: 1
Total Number of designed primers: 5
Number of PGTM primers:     1
Number of PMTM primers:     4

Enzyme Id:  K08247
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103318942     NCBI Gene Symbol: LOC103318942
Gene Aliases
Gene description & Other designations Description:   methionine S-methyltransferase      Other designations:   methionine S-methyltransferase
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 446280 ...... 453973
CDS Sequence 103318942
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TTGAAA)2
Repeat start & end within CDS Repeat start: 962     Repeat end: 973
Forward primer Primer sequence:   ATTCTCCAGGCTGCAGACAC     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GTTGGCGAAGTTGACAGCTG     Tm(°C): 60.041     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 924     End: 1113     Product size (bp): 190
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Enzyme Id:  K08247
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103318942     NCBI Gene Symbol: LOC103318942
Gene Aliases
Gene description & Other designations Description:   methionine S-methyltransferase      Other designations:   methionine S-methyltransferase
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 446280 ...... 453973
CDS Sequence 103318942
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GGT)3
Repeat start & end within CDS Repeat start: 1599     Repeat end: 1607
Forward primer Primer sequence:   GATGAACATCTGACCCGCCA     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGACTCCATTGGAACCTGGG     Tm(°C): 60.034     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 1437     End: 1749     Product size (bp): 313
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Enzyme Id:  K08247
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103318942     NCBI Gene Symbol: LOC103318942
Gene Aliases
Gene description & Other designations Description:   methionine S-methyltransferase      Other designations:   methionine S-methyltransferase
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 446280 ...... 453973
CDS Sequence 103318942
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GTG)3
Repeat start & end within CDS Repeat start: 3002     Repeat end: 3010
Forward primer Primer sequence:   AGGAGATCTGCGGGATGCTA     Tm(°C): 60.179     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGCAATGGTGAACCGACAGT     Tm(°C): 59.891     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 2894     End: 3221     Product size (bp): 328
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Enzyme Id:  K08247
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103318942     NCBI Gene Symbol: LOC103318942
Gene Aliases
Gene description & Other designations Description:   methionine S-methyltransferase      Other designations:   methionine S-methyltransferase
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 446280 ...... 453973
CDS Sequence 103318942
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CTGGA)2
Repeat start & end within CDS Repeat start: 3183     Repeat end: 3192
Forward primer Primer sequence:   AGGAGATCTGCGGGATGCTA     Tm(°C): 60.179     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGCAATGGTGAACCGACAGT     Tm(°C): 59.891     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 2894     End: 3221     Product size (bp): 328
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Enzyme Id:  K08247
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103318942     NCBI Gene Symbol: LOC103318942
Gene Aliases
Gene description & Other designations Description:   methionine S-methyltransferase      Other designations:   methionine S-methyltransferase
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   12
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 446280 ...... 453973
CDS Sequence 103318942
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   ATACGGCGCCTTGAGATCAG     Tm(°C): 59.967     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCACAACCAAGCTCAGCAAC     Tm(°C): 59.969     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 74     End: 391     Product size (bp): 318
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