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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     2
Number of PMTM primers:     1

Enzyme Id:  K08099

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108206898     NCBI Gene Symbol: LOC108206898
Gene Aliases DCAR_005858
Gene description & Other designations Description:   chlorophyllase-1; chloroplastic-like      Other designations:   chlorophyllase-1; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_030382.1      Gene Start and end within genomic accession: 19001405 ...... 19003462
CDS Sequence 108206898
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CTGGA)2
Repeat start & end within CDS Repeat start: 313     Repeat end: 322
Forward primer Primer sequence:   TGCGGAGACCAGGAGTTAGA     Tm(°C): 59.961     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CTGTGACCTCCGACTGCAAT     Tm(°C): 60.037     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 276     End: 397     Product size (bp): 122
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Enzyme Id:  K08099

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108206898     NCBI Gene Symbol: LOC108206898
Gene Aliases DCAR_005858
Gene description & Other designations Description:   chlorophyllase-1; chloroplastic-like      Other designations:   chlorophyllase-1; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_030382.1      Gene Start and end within genomic accession: 19001405 ...... 19003462
CDS Sequence 108206898
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   ATTGCAGTCGGAGGTCACAG     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCAACACTAGGTTCCTCCCC     Tm(°C): 60.035     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 378     End: 877     Product size (bp): 500
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Enzyme Id:  K08099

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108193833     NCBI Gene Symbol: LOC108193833
Gene Aliases DCAR_024190
Gene description & Other designations Description:   chlorophyllase-2; chloroplastic      Other designations:   chlorophyllase-2; chloroplastic
Chromosome, Strand & Exon count Chromosome:   7     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_030387.1      Gene Start and end within genomic accession: 10535350 ...... 10536964
CDS Sequence 108193833
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GGTTGCTGGACCGGATACAA     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACGTATGTCAGCACTGGTGG     Tm(°C): 60.037     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 293     End: 562     Product size (bp): 270
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