image


Statistics

Number of enzymes: 1
Total Number of designed primers: 17
Number of PGTM primers:     3
Number of PMTM primers:     14
Number of Failed designed PMTM primers: 1

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101244270     NCBI Gene Symbol: LOC101244270
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 45-like      Other designations:   pre-mRNA-processing protein 45-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 101244270
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CCAAA)2
Repeat start & end within CDS Repeat start: 44     Repeat end: 53
Forward primer Primer sequence:   GCTGTCGAGCGCATTGC     Tm(°C): 59.94     GC (%): 64.706     Size: 17
Reverse primer Primer sequence:   TGGACGCCATGATGCTCTTT     Tm(°C): 60.035     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 26     End: 197     Product size (bp): 172
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101244270     NCBI Gene Symbol: LOC101244270
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 45-like      Other designations:   pre-mRNA-processing protein 45-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 101244270
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GTCGA)2
Repeat start & end within CDS Repeat start: 127     Repeat end: 136
Forward primer Primer sequence:   AGCAGAAAGGACCACGAGTG     Tm(°C): 59.966     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGGACGCCATGATGCTCTTT     Tm(°C): 60.035     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 91     End: 197     Product size (bp): 107
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101244270     NCBI Gene Symbol: LOC101244270
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 45-like      Other designations:   pre-mRNA-processing protein 45-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 101244270
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (CCCGCC)2
Repeat start & end within CDS Repeat start: 726     Repeat end: 737
Forward primer Primer sequence:   GCTGCGCAGAAACCAAAGAA     Tm(°C): 59.969     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TCTACCATCCGCAGCCAATC     Tm(°C): 59.893     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 534     End: 872     Product size (bp): 339
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101244270     NCBI Gene Symbol: LOC101244270
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 45-like      Other designations:   pre-mRNA-processing protein 45-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 101244270
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (GC)4
Repeat start & end within CDS Repeat start: 1234     Repeat end: 1241
Forward primer Primer sequence:   AGAGGAAGTTCAGCAGCGTC     Tm(°C): 60.038     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCCATGTTCTTCTTGCGCAG     Tm(°C): 59.83     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 950     End: 1288     Product size (bp): 339
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101244270     NCBI Gene Symbol: LOC101244270
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 45-like      Other designations:   pre-mRNA-processing protein 45-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 101244270
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TGA)3
Repeat start & end within CDS Repeat start: 1518     Repeat end: 1526
Forward primer Primer sequence:   CGCGACTTTTCAACCAGAGC     Tm(°C): 60.111     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGTGATCGTGACGAGCGTAT     Tm(°C): 59.971     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1399     End: 1712     Product size (bp): 314
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101244270     NCBI Gene Symbol: LOC101244270
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 45-like      Other designations:   pre-mRNA-processing protein 45-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 101244270
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CTTTT)2
Repeat start & end within CDS Repeat start: 1820     Repeat end: 1829
Forward primer Primer sequence:   ATACGCTCGTCACGATCACC     Tm(°C): 59.971     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGCTGCCCATGAACCAATCT     Tm(°C): 59.96     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 1693     End: 1929     Product size (bp): 237
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101244270     NCBI Gene Symbol: LOC101244270
Gene Aliases
Gene description & Other designations Description:   pre-mRNA-processing protein 45-like      Other designations:   pre-mRNA-processing protein 45-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 101244270
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GGATTTAATCCCGTTGCGCC     Tm(°C): 59.97     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCCATCTGATTGGCTGGTGT     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 404     End: 616     Product size (bp): 213
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101256945     NCBI Gene Symbol: LOC101256945
Gene Aliases
Gene description & Other designations Description:   SNW/SKI-interacting protein A      Other designations:   SNW/SKI-interacting protein A
Chromosome, Strand & Exon count Chromosome:   12     Strand:   plus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_015449.3      Gene Start and end within genomic accession: 1799812 ...... 1803280
CDS Sequence 101256945
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CAGAA)2agccagagaagcagttgcaatgcggtcaaaggttcagaaaga(GAT)
3gaaagaaaaggagaagaaagagatagaacttcgggagttggccc(GCAAG)2
Repeat start & end within CDS Repeat start: 911     Repeat end: 1025
Forward primer Primer sequence:   TCCCCGTCCAGTTACAGTGA     Tm(°C): 60.179     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AAGGTACATGTGCAGCAGCT     Tm(°C): 59.963     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 728     End: 1071     Product size (bp): 344
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101256945     NCBI Gene Symbol: LOC101256945
Gene Aliases
Gene description & Other designations Description:   SNW/SKI-interacting protein A      Other designations:   SNW/SKI-interacting protein A
Chromosome, Strand & Exon count Chromosome:   12     Strand:   plus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_015449.3      Gene Start and end within genomic accession: 1799812 ...... 1803280
CDS Sequence 101256945
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GAGAGG)3(GGAGA)2*
Repeat start & end within CDS Repeat start: 1204     Repeat end: 1224
Forward primer Primer sequence:   TGCCCAAAGAATCAAGGGGG     Tm(°C): 60.252     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GAGTGGGCTGAGCGGTAAAT     Tm(°C): 60.108     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1132     End: 1452     Product size (bp): 321
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101256945     NCBI Gene Symbol: LOC101256945
Gene Aliases
Gene description & Other designations Description:   SNW/SKI-interacting protein A      Other designations:   SNW/SKI-interacting protein A
Chromosome, Strand & Exon count Chromosome:   12     Strand:   plus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_015449.3      Gene Start and end within genomic accession: 1799812 ...... 1803280
CDS Sequence 101256945
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CGGGTGCAAAGGAGAGGATT     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACCTTTGACCGCATTGCAAC     Tm(°C): 59.968     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 601     End: 952     Product size (bp): 352
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101262885     NCBI Gene Symbol: LOC101262885
Gene Aliases
Gene description & Other designations Description:   SNW/SKI-interacting protein-like      Other designations:   SNW/SKI-interacting protein-like
Chromosome, Strand & Exon count Chromosome:   11     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 8034375 ...... 8036425
CDS Sequence 101262885
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TACGA)2
Repeat start & end within CDS Repeat start: 67     Repeat end: 76
Forward primer Primer sequence:   TGCCTGTTACTGTGGACGAA     Tm(°C): 59.246     GC (%): 50     Size: 20
Reverse primer Primer sequence:   GGGTGGCTCCATAGGGTCTA     Tm(°C): 60.105     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 31     End: 362     Product size (bp): 332
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101262885     NCBI Gene Symbol: LOC101262885
Gene Aliases
Gene description & Other designations Description:   SNW/SKI-interacting protein-like      Other designations:   SNW/SKI-interacting protein-like
Chromosome, Strand & Exon count Chromosome:   11     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 8034375 ...... 8036425
CDS Sequence 101262885
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (AGA)3
Repeat start & end within CDS Repeat start: 176     Repeat end: 184
Forward primer Primer sequence:   GCGAGACAAGCCGAAAACTC     Tm(°C): 59.836     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGGTGGCTCCATAGGGTCTA     Tm(°C): 60.105     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 78     End: 362     Product size (bp): 285
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101262885     NCBI Gene Symbol: LOC101262885
Gene Aliases
Gene description & Other designations Description:   SNW/SKI-interacting protein-like      Other designations:   SNW/SKI-interacting protein-like
Chromosome, Strand & Exon count Chromosome:   11     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 8034375 ...... 8036425
CDS Sequence 101262885
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (AGC)3
Repeat start & end within CDS Repeat start: 299     Repeat end: 307
Forward primer Primer sequence:   GCGAGACAAGCCGAAAACTC     Tm(°C): 59.836     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGGTGGCTCCATAGGGTCTA     Tm(°C): 60.105     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 78     End: 362     Product size (bp): 285
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101262885     NCBI Gene Symbol: LOC101262885
Gene Aliases
Gene description & Other designations Description:   SNW/SKI-interacting protein-like      Other designations:   SNW/SKI-interacting protein-like
Chromosome, Strand & Exon count Chromosome:   11     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 8034375 ...... 8036425
CDS Sequence 101262885
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CTC)3
Repeat start & end within CDS Repeat start: 425     Repeat end: 433
Forward primer Primer sequence:   TGGAGCCACCCAAGTTCAAG     Tm(°C): 60.179     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCCAACTCCTGAAGCTCCAT     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 352     End: 700     Product size (bp): 349
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101262885     NCBI Gene Symbol: LOC101262885
Gene Aliases
Gene description & Other designations Description:   SNW/SKI-interacting protein-like      Other designations:   SNW/SKI-interacting protein-like
Chromosome, Strand & Exon count Chromosome:   11     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 8034375 ...... 8036425
CDS Sequence 101262885
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (AG)4
Repeat start & end within CDS Repeat start: 659     Repeat end: 666
Forward primer Primer sequence:   GCATTCTCCTCCTCGTCCTG     Tm(°C): 59.896     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GCCAACTCCTGAAGCTCCAT     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 419     End: 700     Product size (bp): 282
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101262885     NCBI Gene Symbol: LOC101262885
Gene Aliases
Gene description & Other designations Description:   SNW/SKI-interacting protein-like      Other designations:   SNW/SKI-interacting protein-like
Chromosome, Strand & Exon count Chromosome:   11     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 8034375 ...... 8036425
CDS Sequence 101262885
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GA)4agataagcaaggagcgacatc(AGGAG)2
Repeat start & end within CDS Repeat start: 771     Repeat end: 809
Forward primer Primer sequence:   ATGGAGCTTCAGGAGTTGGC     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCATCCCAAGAGCGACCTTT     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 681     End: 906     Product size (bp): 226
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101262885     NCBI Gene Symbol: LOC101262885
Gene Aliases
Gene description & Other designations Description:   SNW/SKI-interacting protein-like      Other designations:   SNW/SKI-interacting protein-like
Chromosome, Strand & Exon count Chromosome:   11     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 8034375 ...... 8036425
CDS Sequence 101262885
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TTTGG)2
Repeat start & end within CDS Repeat start: 1198     Repeat end: 1207
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
JBrowse View      JBrowse

Enzyme Id:  K06063
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   101262885     NCBI Gene Symbol: LOC101262885
Gene Aliases
Gene description & Other designations Description:   SNW/SKI-interacting protein-like      Other designations:   SNW/SKI-interacting protein-like
Chromosome, Strand & Exon count Chromosome:   11     Strand:   plus     Exon count:   1
Gene Location within genomic sequence Genomic accession No. NC_015448.3      Gene Start and end within genomic accession: 8034375 ...... 8036425
CDS Sequence 101262885
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   ATGGAGCTTCAGGAGTTGGC     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCATCCCAAGAGCGACCTTT     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 681     End: 906     Product size (bp): 226
JBrowse View      JBrowse