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Statistics

Number of enzymes: 1
Total Number of designed primers: 2
Number of PGTM primers:     1
Number of PMTM primers:     1

Enzyme Id:  K05581

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668032     NCBI Gene Symbol: ndhJ
Gene Aliases CP95_p064
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit J      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 50627 ...... 51103
CDS Sequence 18668032
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TATATG)2
Repeat start & end within CDS Repeat start: 128     Repeat end: 139
Forward primer Primer sequence:   AGGGTCGTTTGTCTGCTTGG     Tm(°C): 60.535     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCGCACTTTTCCAAACCCAG     Tm(°C): 59.968     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 4     End: 321     Product size (bp): 318
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Enzyme Id:  K05581

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668032     NCBI Gene Symbol: ndhJ
Gene Aliases CP95_p064
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit J      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 50627 ...... 51103
CDS Sequence 18668032
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TTTGCCCCAAGGAGGAATCC     Tm(°C): 59.959     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACGTTTCAGGCGTGGATGAT     Tm(°C): 60.036     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 264     End: 389     Product size (bp): 126
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