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Statistics

Number of enzymes: 1
Total Number of designed primers: 2
Number of PGTM primers:     1
Number of PMTM primers:     1
Number of Failed designed PMTM primers: 1

Enzyme Id:  K05580

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668095     NCBI Gene Symbol: ndhI
Gene Aliases CP95_p011
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit I      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 121934 ...... 122449
CDS Sequence 18668095
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GAATT)2
Repeat start & end within CDS Repeat start: 400     Repeat end: 409
Forward primer Primer sequence:   CCGCGGTCGAATCCATTTTG     Tm(°C): 60.248     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGTAAACGACCCAAAGCAATT     Tm(°C): 57.346     GC (%): 42.857     Size: 21
Primer start, end within sequence and product size Start: 158     End: 436     Product size (bp): 279
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Enzyme Id:  K05580

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668095     NCBI Gene Symbol: ndhI
Gene Aliases CP95_p011
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit I      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 121934 ...... 122449
CDS Sequence 18668095
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Mono     Repeat sequence: (A)12
Repeat start & end within CDS Repeat start: 488     Repeat end: 499
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K05580

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668095     NCBI Gene Symbol: ndhI
Gene Aliases CP95_p011
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit I      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 121934 ...... 122449
CDS Sequence 18668095
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CAAACAGTACGAGCTGCAAGG     Tm(°C): 59.805     GC (%): 52.381     Size: 21
Reverse primer Primer sequence:   CAAAATGGATTCGACCGCGG     Tm(°C): 60.248     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 39     End: 177     Product size (bp): 139
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