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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     1
Number of PMTM primers:     2

Enzyme Id:  K05579

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668097     NCBI Gene Symbol: ndhH
Gene Aliases CP95_p009
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 7      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 124753 ...... 125934
CDS Sequence 18668097
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (AGAGAA)2
Repeat start & end within CDS Repeat start: 406     Repeat end: 417
Forward primer Primer sequence:   ATGGCGGATATTGGTGCACA     Tm(°C): 60.107     GC (%): 50     Size: 20
Reverse primer Primer sequence:   ATTCTCATACCGGTGGCAGC     Tm(°C): 60.179     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 363     End: 457     Product size (bp): 95
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Enzyme Id:  K05579

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668097     NCBI Gene Symbol: ndhH
Gene Aliases CP95_p009
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 7      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 124753 ...... 125934
CDS Sequence 18668097
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GGAAT)2
Repeat start & end within CDS Repeat start: 903     Repeat end: 912
Forward primer Primer sequence:   TGGAAGGAATTCCAGGGGGA     Tm(°C): 60.179     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TTTGGGGCTTCGACTCTCAC     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 835     End: 1000     Product size (bp): 166
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Enzyme Id:  K05579

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668097     NCBI Gene Symbol: ndhH
Gene Aliases CP95_p009
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 7      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 124753 ...... 125934
CDS Sequence 18668097
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   ACCACCCATCAATGCATGGT     Tm(°C): 59.958     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TGTGCACCAATATCCGCCAT     Tm(°C): 60.107     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 52     End: 382     Product size (bp): 331
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