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Statistics

Number of enzymes: 1
Total Number of designed primers: 7
Number of PGTM primers:     1
Number of PMTM primers:     6
Number of Failed designed PMTM primers: 2

Enzyme Id:  K05577
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668087     NCBI Gene Symbol: ndhF
Gene Aliases CP95_p018
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 5      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 112207 ...... 114444
CDS Sequence 18668087
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TTA)3
Repeat start & end within CDS Repeat start: 565     Repeat end: 573
Forward primer Primer sequence:   TGGTTCACACGACCTAGTGC     Tm(°C): 59.967     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TTGGGCGGATTTTGCAACTG     Tm(°C): 59.967     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 492     End: 728     Product size (bp): 237
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Enzyme Id:  K05577
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668087     NCBI Gene Symbol: ndhF
Gene Aliases CP95_p018
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 5      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 112207 ...... 114444
CDS Sequence 18668087
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AGCTT)2
Repeat start & end within CDS Repeat start: 952     Repeat end: 961
Forward primer Primer sequence:   CAGTTGCAAAATCCGCCCAA     Tm(°C): 59.967     GC (%): 50     Size: 20
Reverse primer Primer sequence:   AGCAACTCGATAAGACCCCA     Tm(°C): 58.431     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 709     End: 1025     Product size (bp): 317
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Enzyme Id:  K05577
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668087     NCBI Gene Symbol: ndhF
Gene Aliases CP95_p018
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 5      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 112207 ...... 114444
CDS Sequence 18668087
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TAAAAT)2
Repeat start & end within CDS Repeat start: 1944     Repeat end: 1955
Forward primer Primer sequence:   TCACAAATGCAACCTTTTCCAT     Tm(°C): 57.314     GC (%): 36.364     Size: 22
Reverse primer Primer sequence:   CGCAATACCGACTCCATTCG     Tm(°C): 59.147     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1813     End: 2123     Product size (bp): 311
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Enzyme Id:  K05577
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668087     NCBI Gene Symbol: ndhF
Gene Aliases CP95_p018
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 5      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 112207 ...... 114444
CDS Sequence 18668087
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TTA)3
Repeat start & end within CDS Repeat start: 2212     Repeat end: 2220
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K05577
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668087     NCBI Gene Symbol: ndhF
Gene Aliases CP95_p018
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 5      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 112207 ...... 114444
CDS Sequence 18668087
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TGGTTCACACGACCTAGTGC     Tm(°C): 59.967     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TTGGGCGGATTTTGCAACTG     Tm(°C): 59.967     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 492     End: 728     Product size (bp): 237
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Enzyme Id:  K05577
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103323521     NCBI Gene Symbol: LOC103323521
Gene Aliases
Gene description & Other designations Description:   NAD(P)H-quinone oxidoreductase subunit 5; chloroplastic      Other designations:   NAD(P)H-quinone oxidoreductase subunit 5; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 35637180 ...... 35640264
CDS Sequence 103323521
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TTA)3
Repeat start & end within CDS Repeat start: 565     Repeat end: 573
Forward primer Primer sequence:   TGGTTCACACGACCTAGTGC     Tm(°C): 59.967     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TTGGGCGGATTTTGCAACTG     Tm(°C): 59.967     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 492     End: 728     Product size (bp): 237
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Enzyme Id:  K05577
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103323521     NCBI Gene Symbol: LOC103323521
Gene Aliases
Gene description & Other designations Description:   NAD(P)H-quinone oxidoreductase subunit 5; chloroplastic      Other designations:   NAD(P)H-quinone oxidoreductase subunit 5; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 35637180 ...... 35640264
CDS Sequence 103323521
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AGCTT)2
Repeat start & end within CDS Repeat start: 952     Repeat end: 961
Forward primer Primer sequence:   CAGTTGCAAAATCCGCCCAA     Tm(°C): 59.967     GC (%): 50     Size: 20
Reverse primer Primer sequence:   AGCAACTCGATAAGACCCCA     Tm(°C): 58.431     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 709     End: 1025     Product size (bp): 317
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Enzyme Id:  K05577
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103323521     NCBI Gene Symbol: LOC103323521
Gene Aliases
Gene description & Other designations Description:   NAD(P)H-quinone oxidoreductase subunit 5; chloroplastic      Other designations:   NAD(P)H-quinone oxidoreductase subunit 5; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 35637180 ...... 35640264
CDS Sequence 103323521
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TAAAAT)2
Repeat start & end within CDS Repeat start: 1944     Repeat end: 1955
Forward primer Primer sequence:   TCACAAATGCAACCTTTTCCAT     Tm(°C): 57.314     GC (%): 36.364     Size: 22
Reverse primer Primer sequence:   CGCAATACCGACTCCATTCG     Tm(°C): 59.147     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1813     End: 2123     Product size (bp): 311
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Enzyme Id:  K05577
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103323521     NCBI Gene Symbol: LOC103323521
Gene Aliases
Gene description & Other designations Description:   NAD(P)H-quinone oxidoreductase subunit 5; chloroplastic      Other designations:   NAD(P)H-quinone oxidoreductase subunit 5; chloroplastic
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 35637180 ...... 35640264
CDS Sequence 103323521
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (AAT)3
Repeat start & end within CDS Repeat start: 2157     Repeat end: 2165
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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