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Statistics

Number of enzymes: 1
Total Number of designed primers: 1
Number of PGTM primers:     1
Number of Failed designed PMTM primers: 1

Enzyme Id:  K05576

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668093     NCBI Gene Symbol: ndhE
Gene Aliases CP95_p013
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 4L      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 120444 ...... 120749
CDS Sequence 18668093
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tetra     Repeat sequence: (ATCA)3
Repeat start & end within CDS Repeat start: 277     Repeat end: 288
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K05576

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668093     NCBI Gene Symbol: ndhE
Gene Aliases CP95_p013
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 4L      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 120444 ...... 120749
CDS Sequence 18668093
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GCATGTACTTGTTTTGAGTGCCT     Tm(°C): 59.995     GC (%): 43.478     Size: 23
Reverse primer Primer sequence:   ATAGCCGGACCAATAGCTGC     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 11     End: 238     Product size (bp): 228
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