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Statistics

Number of enzymes: 1
Total Number of designed primers: 4
Number of PGTM primers:     1
Number of PMTM primers:     3

Enzyme Id:  K05575
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668091     NCBI Gene Symbol: ndhD
Gene Aliases CP95_p015
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 4      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 118316 ...... 119818
CDS Sequence 18668091
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TATATG)2
Repeat start & end within CDS Repeat start: 108     Repeat end: 119
Forward primer Primer sequence:   TTGCCAATATTCGCGGGTTC     Tm(°C): 59.264     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CGGGAATCTCGAGTAACCGG     Tm(°C): 59.97     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 36     End: 334     Product size (bp): 299
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Enzyme Id:  K05575
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668091     NCBI Gene Symbol: ndhD
Gene Aliases CP95_p015
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 4      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 118316 ...... 119818
CDS Sequence 18668091
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GCGCT)2
Repeat start & end within CDS Repeat start: 1025     Repeat end: 1034
Forward primer Primer sequence:   CAGGACTCAACGGAGCCATT     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCGCCCATTTCGTCGAGATA     Tm(°C): 59.968     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 976     End: 1096     Product size (bp): 121
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Enzyme Id:  K05575
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668091     NCBI Gene Symbol: ndhD
Gene Aliases CP95_p015
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 4      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 118316 ...... 119818
CDS Sequence 18668091
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TCGATC)2
Repeat start & end within CDS Repeat start: 1393     Repeat end: 1404
Forward primer Primer sequence:   TCTTGCATTACCGGGCATGA     Tm(°C): 59.746     GC (%): 50     Size: 20
Reverse primer Primer sequence:   AGCTTCGACCTTGTCAACTGA     Tm(°C): 59.587     GC (%): 47.619     Size: 21
Primer start, end within sequence and product size Start: 1154     End: 1475     Product size (bp): 322
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Enzyme Id:  K05575
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668091     NCBI Gene Symbol: ndhD
Gene Aliases CP95_p015
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 4      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 118316 ...... 119818
CDS Sequence 18668091
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CAGGACTCAACGGAGCCATT     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCGCCCATTTCGTCGAGATA     Tm(°C): 59.968     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 976     End: 1096     Product size (bp): 121
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