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Statistics

Number of enzymes: 1
Total Number of designed primers: 10
Number of PGTM primers:     1
Number of PMTM primers:     9

Enzyme Id:  K05573

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   107881997     NCBI Gene Symbol: LOC107881997
Gene Aliases
Gene description & Other designations Description:   NAD(P)H-quinone oxidoreductase subunit 2 B; chloroplastic      Other designations:   NAD(P)H-quinone oxidoreductase subunit 2 B; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 107881997
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TTC)3
Repeat start & end within CDS Repeat start: 128     Repeat end: 136
Forward primer Primer sequence:   GCCTTTCATTTGCTTCTCTTCG     Tm(°C): 58.504     GC (%): 45.455     Size: 22
Reverse primer Primer sequence:   CATCGGAACAATAGGGCCGT     Tm(°C): 60.179     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 60     End: 235     Product size (bp): 176
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Enzyme Id:  K05573

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   107881997     NCBI Gene Symbol: LOC107881997
Gene Aliases
Gene description & Other designations Description:   NAD(P)H-quinone oxidoreductase subunit 2 B; chloroplastic      Other designations:   NAD(P)H-quinone oxidoreductase subunit 2 B; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 107881997
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GCTTCA)2gccactcgaattttcgatattcctttttatttctcatcaaacgaat
ggca(TCT)3
Repeat start & end within CDS Repeat start: 826     Repeat end: 896
Forward primer Primer sequence:   ACGAAGGATCTCCCACTCCA     Tm(°C): 59.959     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGACGAATACGCAAGCATACG     Tm(°C): 59.811     GC (%): 52.381     Size: 21
Primer start, end within sequence and product size Start: 769     End: 983     Product size (bp): 215
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Enzyme Id:  K05573

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   107881997     NCBI Gene Symbol: LOC107881997
Gene Aliases
Gene description & Other designations Description:   NAD(P)H-quinone oxidoreductase subunit 2 B; chloroplastic      Other designations:   NAD(P)H-quinone oxidoreductase subunit 2 B; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 107881997
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (CT)4
Repeat start & end within CDS Repeat start: 1184     Repeat end: 1191
Forward primer Primer sequence:   TGGTCTACGTACCGGAACTG     Tm(°C): 58.832     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCTGCCATCCACACCAGAAT     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1115     End: 1278     Product size (bp): 164
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Enzyme Id:  K05573

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   107881997     NCBI Gene Symbol: LOC107881997
Gene Aliases
Gene description & Other designations Description:   NAD(P)H-quinone oxidoreductase subunit 2 B; chloroplastic      Other designations:   NAD(P)H-quinone oxidoreductase subunit 2 B; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 107881997
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CTTCAGCTTCAGCCACTCGA     Tm(°C): 60.038     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCTGCCATCCACACCAGAAT     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 826     End: 1278     Product size (bp): 453
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Enzyme Id:  K05573

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668079     NCBI Gene Symbol: ndhB
Gene Aliases CP95_p021
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 2      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 96712 ...... 98924
CDS Sequence 18668079
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TTC)3
Repeat start & end within CDS Repeat start: 128     Repeat end: 136
Forward primer Primer sequence:   GCCTTTCATTTGCTTCTCTTCG     Tm(°C): 58.504     GC (%): 45.455     Size: 22
Reverse primer Primer sequence:   CATCGGAACAATAGGGCCGT     Tm(°C): 60.179     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 60     End: 235     Product size (bp): 176
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Enzyme Id:  K05573

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668079     NCBI Gene Symbol: ndhB
Gene Aliases CP95_p021
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 2      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 96712 ...... 98924
CDS Sequence 18668079
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GCTTCA)2gccactcgaattttcgatattcctttttatttctcatcaaacgaat
ggca(TCT)3
Repeat start & end within CDS Repeat start: 826     Repeat end: 896
Forward primer Primer sequence:   ACGAAGGATCTCCCACTCCA     Tm(°C): 59.959     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGACGAATACGCAAGCATACG     Tm(°C): 59.811     GC (%): 52.381     Size: 21
Primer start, end within sequence and product size Start: 769     End: 983     Product size (bp): 215
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Enzyme Id:  K05573

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668079     NCBI Gene Symbol: ndhB
Gene Aliases CP95_p021
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 2      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   minus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 96712 ...... 98924
CDS Sequence 18668079
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (CT)4
Repeat start & end within CDS Repeat start: 1184     Repeat end: 1191
Forward primer Primer sequence:   TGGTCTACGTACCGGAACTG     Tm(°C): 58.832     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCTGCCATCCACACCAGAAT     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1115     End: 1278     Product size (bp): 164
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Enzyme Id:  K05573

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668106     NCBI Gene Symbol: ndhB
Gene Aliases CP95_p005
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 2      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   plus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 144587 ...... 146799
CDS Sequence 18668106
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (TTC)3
Repeat start & end within CDS Repeat start: 128     Repeat end: 136
Forward primer Primer sequence:   GCCTTTCATTTGCTTCTCTTCG     Tm(°C): 58.504     GC (%): 45.455     Size: 22
Reverse primer Primer sequence:   CATCGGAACAATAGGGCCGT     Tm(°C): 60.179     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 60     End: 235     Product size (bp): 176
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Enzyme Id:  K05573

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668106     NCBI Gene Symbol: ndhB
Gene Aliases CP95_p005
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 2      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   plus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 144587 ...... 146799
CDS Sequence 18668106
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GCTTCA)2gccactcgaattttcgatattcctttttatttctcatcaaacgaat
ggca(TCT)3
Repeat start & end within CDS Repeat start: 826     Repeat end: 896
Forward primer Primer sequence:   ACGAAGGATCTCCCACTCCA     Tm(°C): 59.959     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGACGAATACGCAAGCATACG     Tm(°C): 59.811     GC (%): 52.381     Size: 21
Primer start, end within sequence and product size Start: 769     End: 983     Product size (bp): 215
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Enzyme Id:  K05573

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   18668106     NCBI Gene Symbol: ndhB
Gene Aliases CP95_p005
Gene description & Other designations Description:   NADH-plastoquinone oxidoreductase subunit 2      Other designations:   
Chromosome, Strand & Exon count Chromosome:        Strand:   plus     Exon count:   0
Gene Location within genomic sequence Genomic accession No. NC_023798.1      Gene Start and end within genomic accession: 144587 ...... 146799
CDS Sequence 18668106
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (CT)4
Repeat start & end within CDS Repeat start: 1184     Repeat end: 1191
Forward primer Primer sequence:   TGGTCTACGTACCGGAACTG     Tm(°C): 58.832     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCTGCCATCCACACCAGAAT     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1115     End: 1278     Product size (bp): 164
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