PlantPathMarks Database

Statistics

Number of enzymes: 1

Total Number of designed primers: 6

Number of PGTM primers: 1

Number of PMTM primers: 5

Enzyme Id: K04718
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 103324637 NCBI Gene Symbol: LOC103324637
Gene Aliases
Gene description & Other designations	Description: sphingosine kinase 1-like Other designations: sphingosine kinase 1-like\|sphingosine kinase 2-like
Chromosome, Strand & Exon count	Chromosome: LG3 Strand: minus Exon count: 11
Gene Location within genomic sequence	Genomic accession No. NC_024128.1 Gene Start and end within genomic accession: 1596779 ...... 1600944
CDS Sequence	103324637
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Hexa Repeat sequence: (GATCAG)2
Repeat start & end within CDS	Repeat start: 177 Repeat end: 188
Forward primer	Primer sequence: AACGGAGCTTGATCGTGGAG Tm(°C): 60.109 GC (%): 55 Size: 20
Reverse primer	Primer sequence: AGCCTATGCGAGTCCTCAGA Tm(°C): 60.106 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 121 End: 292 Product size (bp): 172
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Enzyme Id: K04718
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 103324637 NCBI Gene Symbol: LOC103324637
Gene Aliases
Gene description & Other designations	Description: sphingosine kinase 1-like Other designations: sphingosine kinase 1-like\|sphingosine kinase 2-like
Chromosome, Strand & Exon count	Chromosome: LG3 Strand: minus Exon count: 11
Gene Location within genomic sequence	Genomic accession No. NC_024128.1 Gene Start and end within genomic accession: 1596779 ...... 1600944
CDS Sequence	103324637
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Di Repeat sequence: (CT)4
Repeat start & end within CDS	Repeat start: 269 Repeat end: 276
Forward primer	Primer sequence: AACGGAGCTTGATCGTGGAG Tm(°C): 60.109 GC (%): 55 Size: 20
Reverse primer	Primer sequence: CCGCCCCAGAGAATCAATGT Tm(°C): 60.107 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 121 End: 335 Product size (bp): 215
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Enzyme Id: K04718
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 103324637 NCBI Gene Symbol: LOC103324637
Gene Aliases
Gene description & Other designations	Description: sphingosine kinase 1-like Other designations: sphingosine kinase 1-like\|sphingosine kinase 2-like
Chromosome, Strand & Exon count	Chromosome: LG3 Strand: minus Exon count: 11
Gene Location within genomic sequence	Genomic accession No. NC_024128.1 Gene Start and end within genomic accession: 1596779 ...... 1600944
CDS Sequence	103324637
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Di Repeat sequence: (GA)4
Repeat start & end within CDS	Repeat start: 574 Repeat end: 581
Forward primer	Primer sequence: AGTGGGGATGGAATCTTGGT Tm(°C): 58.314 GC (%): 50 Size: 20
Reverse primer	Primer sequence: TCCCCTGCCATATGGTAGCT Tm(°C): 60.104 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 531 End: 756 Product size (bp): 226
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Enzyme Id: K04718
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 103324637 NCBI Gene Symbol: LOC103324637
Gene Aliases
Gene description & Other designations	Description: sphingosine kinase 1-like Other designations: sphingosine kinase 1-like\|sphingosine kinase 2-like
Chromosome, Strand & Exon count	Chromosome: LG3 Strand: minus Exon count: 11
Gene Location within genomic sequence	Genomic accession No. NC_024128.1 Gene Start and end within genomic accession: 1596779 ...... 1600944
CDS Sequence	103324637
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Penta Repeat sequence: (AATGG)2
Repeat start & end within CDS	Repeat start: 637 Repeat end: 646
Forward primer	Primer sequence: GAGAGGATTGGGAAGCTGCA Tm(°C): 59.746 GC (%): 55 Size: 20
Reverse primer	Primer sequence: TCCCCTGCCATATGGTAGCT Tm(°C): 60.104 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 577 End: 756 Product size (bp): 180
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Enzyme Id: K04718
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 103324637 NCBI Gene Symbol: LOC103324637
Gene Aliases
Gene description & Other designations	Description: sphingosine kinase 1-like Other designations: sphingosine kinase 1-like\|sphingosine kinase 2-like
Chromosome, Strand & Exon count	Chromosome: LG3 Strand: minus Exon count: 11
Gene Location within genomic sequence	Genomic accession No. NC_024128.1 Gene Start and end within genomic accession: 1596779 ...... 1600944
CDS Sequence	103324637
	Marker Information
Marker Type	PMTM
Repeat type & sequence	Repeat type: Tri Repeat sequence: (ACA)3
Repeat start & end within CDS	Repeat start: 959 Repeat end: 967
Forward primer	Primer sequence: TTGCATGGGGTTTAGTGGCA Tm(°C): 60.179 GC (%): 50 Size: 20
Reverse primer	Primer sequence: TGAAACGAATGGCCCACTGA Tm(°C): 59.89 GC (%): 50 Size: 20
Primer start, end within sequence and product size	Start: 784 End: 1106 Product size (bp): 323
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Enzyme Id: K04718
	Gene Information
NCBI Gene Accession Number & Symbol	Accession Number: 103324637 NCBI Gene Symbol: LOC103324637
Gene Aliases
Gene description & Other designations	Description: sphingosine kinase 1-like Other designations: sphingosine kinase 1-like\|sphingosine kinase 2-like
Chromosome, Strand & Exon count	Chromosome: LG3 Strand: minus Exon count: 11
Gene Location within genomic sequence	Genomic accession No. NC_024128.1 Gene Start and end within genomic accession: 1596779 ...... 1600944
CDS Sequence	103324637
	Marker Information
Marker Type	PGTM
Repeat type & sequence	Repeat type: Repeat sequence:
Repeat start & end within CDS	Repeat start: Repeat end:
Forward primer	Primer sequence: CCTTGGGGGAGTGAAGACAC Tm(°C): 59.963 GC (%): 60 Size: 20
Reverse primer	Primer sequence: CCTGTGCTCAACCCTGACAT Tm(°C): 59.963 GC (%): 55 Size: 20
Primer start, end within sequence and product size	Start: 1125 End: 1252 Product size (bp): 128
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