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Statistics

Number of enzymes: 1
Total Number of designed primers: 5
Number of PGTM primers:     2
Number of PMTM primers:     3

Enzyme Id:  K04711

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103336548     NCBI Gene Symbol: LOC103336548
Gene Aliases
Gene description & Other designations Description:   alkaline ceramidase 3-like      Other designations:   alkaline ceramidase 3-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   4
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 20883801 ...... 20887518
CDS Sequence 103336548
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GCA)3
Repeat start & end within CDS Repeat start: 252     Repeat end: 260
Forward primer Primer sequence:   GCTTTGAGGCAACGCTTTGA     Tm(°C): 59.969     GC (%): 50     Size: 20
Reverse primer Primer sequence:   CAAGAAGGTGGGCATTGTGC     Tm(°C): 60.038     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 150     End: 356     Product size (bp): 207
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Enzyme Id:  K04711

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103336548     NCBI Gene Symbol: LOC103336548
Gene Aliases
Gene description & Other designations Description:   alkaline ceramidase 3-like      Other designations:   alkaline ceramidase 3-like
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   minus     Exon count:   4
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 20883801 ...... 20887518
CDS Sequence 103336548
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GCTTTGAGGCAACGCTTTGA     Tm(°C): 59.969     GC (%): 50     Size: 20
Reverse primer Primer sequence:   AGACTTCCCTGCACAAGACG     Tm(°C): 59.966     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 150     End: 582     Product size (bp): 433
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Enzyme Id:  K04711

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327002     NCBI Gene Symbol: LOC103327002
Gene Aliases
Gene description & Other designations Description:   alkaline ceramidase 3      Other designations:   alkaline ceramidase 3
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 20833175 ...... 20835924
CDS Sequence 103327002
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TCTTCC)2
Repeat start & end within CDS Repeat start: 353     Repeat end: 364
Forward primer Primer sequence:   AAGGCAGCGGTTTGAGAAGA     Tm(°C): 59.891     GC (%): 50     Size: 20
Reverse primer Primer sequence:   GCCACAGAATGAACAGCAGC     Tm(°C): 60.109     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 158     End: 400     Product size (bp): 243
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Enzyme Id:  K04711

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327002     NCBI Gene Symbol: LOC103327002
Gene Aliases
Gene description & Other designations Description:   alkaline ceramidase 3      Other designations:   alkaline ceramidase 3
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 20833175 ...... 20835924
CDS Sequence 103327002
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (TA)4
Repeat start & end within CDS Repeat start: 475     Repeat end: 482
Forward primer Primer sequence:   GCTGCTGTTCATTCTGTGGC     Tm(°C): 60.109     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GAGCCCGGCAAAACATCAAG     Tm(°C): 60.109     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 381     End: 684     Product size (bp): 304
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Enzyme Id:  K04711

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103327002     NCBI Gene Symbol: LOC103327002
Gene Aliases
Gene description & Other designations Description:   alkaline ceramidase 3      Other designations:   alkaline ceramidase 3
Chromosome, Strand & Exon count Chromosome:   LG3     Strand:   minus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024128.1      Gene Start and end within genomic accession: 20833175 ...... 20835924
CDS Sequence 103327002
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GGATCAAGCTTCTGGGGTCC     Tm(°C): 60.107     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TCTTCTCAAACCGCTGCCTT     Tm(°C): 59.891     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 12     End: 177     Product size (bp): 166
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