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Statistics

Number of enzymes: 1
Total Number of designed primers: 7
Number of PGTM primers:     2
Number of PMTM primers:     5

Enzyme Id:  K04708

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103344063     NCBI Gene Symbol: LOC103344063
Gene Aliases
Gene description & Other designations Description:   3-dehydrosphinganine reductase TSC10A-like      Other designations:   3-dehydrosphinganine reductase TSC10A-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103344063
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CTC)4
Repeat start & end within CDS Repeat start: 34     Repeat end: 45
Forward primer Primer sequence:   CCTCTCTTTCCTCGCCCTTC     Tm(°C): 59.823     GC (%): 60     Size: 20
Reverse primer Primer sequence:   CCTCCTCCACCGCTTTTGAT     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 14     End: 333     Product size (bp): 320
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Enzyme Id:  K04708

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103344063     NCBI Gene Symbol: LOC103344063
Gene Aliases
Gene description & Other designations Description:   3-dehydrosphinganine reductase TSC10A-like      Other designations:   3-dehydrosphinganine reductase TSC10A-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103344063
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TGGTT)2
Repeat start & end within CDS Repeat start: 897     Repeat end: 906
Forward primer Primer sequence:   ATAGCAGAGTCGTCAGGGGT     Tm(°C): 60.033     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAGGCCCTTTTGTTCTGTGC     Tm(°C): 59.967     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 717     End: 982     Product size (bp): 266
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Enzyme Id:  K04708

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103344063     NCBI Gene Symbol: LOC103344063
Gene Aliases
Gene description & Other designations Description:   3-dehydrosphinganine reductase TSC10A-like      Other designations:   3-dehydrosphinganine reductase TSC10A-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103344063
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GTTGAAGTGGTTGCTGCTGG     Tm(°C): 59.969     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAGGCCCTTTTGTTCTGTGC     Tm(°C): 59.967     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 879     End: 982     Product size (bp): 104
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Enzyme Id:  K04708

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103344249     NCBI Gene Symbol: LOC103344249
Gene Aliases
Gene description & Other designations Description:   3-dehydrosphinganine reductase TSC10A-like      Other designations:   3-dehydrosphinganine reductase TSC10A-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103344249
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CTC)4
Repeat start & end within CDS Repeat start: 34     Repeat end: 45
Forward primer Primer sequence:   CCTCTCTTTCCTCGCCCTTC     Tm(°C): 59.823     GC (%): 60     Size: 20
Reverse primer Primer sequence:   CCTCCTCCACCGCTTTTGAT     Tm(°C): 60.035     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 14     End: 333     Product size (bp): 320
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Enzyme Id:  K04708

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103344249     NCBI Gene Symbol: LOC103344249
Gene Aliases
Gene description & Other designations Description:   3-dehydrosphinganine reductase TSC10A-like      Other designations:   3-dehydrosphinganine reductase TSC10A-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103344249
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TGGTT)2
Repeat start & end within CDS Repeat start: 897     Repeat end: 906
Forward primer Primer sequence:   ATAGCAGAGTCGTCAGGGGT     Tm(°C): 60.033     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCATGCCACGTGTGTATACC     Tm(°C): 59.344     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 717     End: 964     Product size (bp): 248
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Enzyme Id:  K04708

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103344249     NCBI Gene Symbol: LOC103344249
Gene Aliases
Gene description & Other designations Description:   3-dehydrosphinganine reductase TSC10A-like      Other designations:   3-dehydrosphinganine reductase TSC10A-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 103344249
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   ATAGCAGAGTCGTCAGGGGT     Tm(°C): 60.033     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCAGCAGCAACCACTTCAAC     Tm(°C): 59.969     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 717     End: 898     Product size (bp): 182
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Enzyme Id:  K04708

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103331440     NCBI Gene Symbol: LOC103331440
Gene Aliases
Gene description & Other designations Description:   3-dehydrosphinganine reductase TSC10A-like      Other designations:   3-dehydrosphinganine reductase TSC10A-like
Chromosome, Strand & Exon count Chromosome:   LG1     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024126.1      Gene Start and end within genomic accession: 13557854 ...... 13559902
CDS Sequence 103331440
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (TGGTT)2
Repeat start & end within CDS Repeat start: 696     Repeat end: 705
Forward primer Primer sequence:   ATAGCAGAGTCGTCAGGGGT     Tm(°C): 60.033     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAGGCCCTTTTGTTCTGTGC     Tm(°C): 59.967     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 516     End: 781     Product size (bp): 266
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