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Statistics

Number of enzymes: 1
Total Number of designed primers: 2
Number of PGTM primers:     1
Number of PMTM primers:     1
Number of Failed designed PMTM primers: 1

Enzyme Id:  K03943

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103328064     NCBI Gene Symbol: LOC103328064
Gene Aliases
Gene description & Other designations Description:   NADH dehydrogenase [ubiquinone] flavoprotein 2; mitochondrial      Other designations:   NADH dehydrogenase [ubiquinone] flavoprotein 2; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 5734728 ...... 5738771
CDS Sequence 103328064
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CAG)3
Repeat start & end within CDS Repeat start: 229     Repeat end: 237
Forward primer Primer sequence:   TCGCTTCCAATCGTCTCCAC     Tm(°C): 60.109     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCCACCTTCGTCCGATTGAA     Tm(°C): 60.037     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 13     End: 355     Product size (bp): 343
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Enzyme Id:  K03943

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103328064     NCBI Gene Symbol: LOC103328064
Gene Aliases
Gene description & Other designations Description:   NADH dehydrogenase [ubiquinone] flavoprotein 2; mitochondrial      Other designations:   NADH dehydrogenase [ubiquinone] flavoprotein 2; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 5734728 ...... 5738771
CDS Sequence 103328064
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CTCGC)2
Repeat start & end within CDS Repeat start: 8     Repeat end: 17
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K03943

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103328064     NCBI Gene Symbol: LOC103328064
Gene Aliases
Gene description & Other designations Description:   NADH dehydrogenase [ubiquinone] flavoprotein 2; mitochondrial      Other designations:   NADH dehydrogenase [ubiquinone] flavoprotein 2; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   minus     Exon count:   10
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 5734728 ...... 5738771
CDS Sequence 103328064
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TTCAATCGGACGAAGGTGGG     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TCACACAGCTTCCCATGCAT     Tm(°C): 59.961     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 336     End: 516     Product size (bp): 181
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