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Statistics

Number of enzymes: 1
Total Number of designed primers: 5
Number of PGTM primers:     1
Number of PMTM primers:     4

Enzyme Id:  K03942

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321985     NCBI Gene Symbol: LOC103321985
Gene Aliases
Gene description & Other designations Description:   NADH dehydrogenase [ubiquinone] flavoprotein 1; mitochondrial      Other designations:   NADH dehydrogenase [ubiquinone] flavoprotein 1; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 20051924 ...... 20055467
CDS Sequence 103321985
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (GATTG)2tc(AATGA)2agaagtctggcctccgtggac(GTG)3
Repeat start & end within CDS Repeat start: 289     Repeat end: 340
Forward primer Primer sequence:   CCCACCTCCAGAGAAAACCC     Tm(°C): 59.962     GC (%): 60     Size: 20
Reverse primer Primer sequence:   TGTGTGGATCATGGCGCATA     Tm(°C): 59.82     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 140     End: 477     Product size (bp): 338
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Enzyme Id:  K03942

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321985     NCBI Gene Symbol: LOC103321985
Gene Aliases
Gene description & Other designations Description:   NADH dehydrogenase [ubiquinone] flavoprotein 1; mitochondrial      Other designations:   NADH dehydrogenase [ubiquinone] flavoprotein 1; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 20051924 ...... 20055467
CDS Sequence 103321985
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (TGGTGC)2
Repeat start & end within CDS Repeat start: 666     Repeat end: 677
Forward primer Primer sequence:   ATGAAGCTGGGTTACTGGGC     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GAAACAGCCACCGTTTCCAC     Tm(°C): 59.97     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 598     End: 820     Product size (bp): 223
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Enzyme Id:  K03942

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321985     NCBI Gene Symbol: LOC103321985
Gene Aliases
Gene description & Other designations Description:   NADH dehydrogenase [ubiquinone] flavoprotein 1; mitochondrial      Other designations:   NADH dehydrogenase [ubiquinone] flavoprotein 1; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 20051924 ...... 20055467
CDS Sequence 103321985
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AGGGA)2
Repeat start & end within CDS Repeat start: 1225     Repeat end: 1234
Forward primer Primer sequence:   CTGAAGGCTGTCCAGTCAGG     Tm(°C): 60.037     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GCTCCCTGATCCTCCTCTCA     Tm(°C): 60.106     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 1092     End: 1425     Product size (bp): 334
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Enzyme Id:  K03942

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321985     NCBI Gene Symbol: LOC103321985
Gene Aliases
Gene description & Other designations Description:   NADH dehydrogenase [ubiquinone] flavoprotein 1; mitochondrial      Other designations:   NADH dehydrogenase [ubiquinone] flavoprotein 1; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 20051924 ...... 20055467
CDS Sequence 103321985
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (TGC)3(GCCTG)2*
Repeat start & end within CDS Repeat start: 1356     Repeat end: 1372
Forward primer Primer sequence:   CTGAAGGCTGTCCAGTCAGG     Tm(°C): 60.037     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GCTCCCTGATCCTCCTCTCA     Tm(°C): 60.106     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 1092     End: 1425     Product size (bp): 334
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Enzyme Id:  K03942

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103321985     NCBI Gene Symbol: LOC103321985
Gene Aliases
Gene description & Other designations Description:   NADH dehydrogenase [ubiquinone] flavoprotein 1; mitochondrial      Other designations:   NADH dehydrogenase [ubiquinone] flavoprotein 1; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 20051924 ...... 20055467
CDS Sequence 103321985
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CCCATCTGGCCTCAAATGGT     Tm(°C): 60.033     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GAAACAGCCACCGTTTCCAC     Tm(°C): 59.97     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 347     End: 820     Product size (bp): 474
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