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Statistics

Number of enzymes: 1
Total Number of designed primers: 2
Number of PGTM primers:     1
Number of PMTM primers:     1

Enzyme Id:  K03941

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335651     NCBI Gene Symbol: LOC103335651
Gene Aliases
Gene description & Other designations Description:   NADH dehydrogenase [ubiquinone] iron-sulfur protein 8; mitochondrial      Other designations:   NADH dehydrogenase [ubiquinone] iron-sulfur protein 8; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   plus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 11049657 ...... 11054181
CDS Sequence 103335651
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (TGAGGC)2acgagaagatggtagccgtaggactactaggta(TGACAT)2(TGA
CA)2*
Repeat start & end within CDS Repeat start: 420     Repeat end: 480
Forward primer Primer sequence:   TCGTGGTGAACATGCCCTAC     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TGTCTCCATTCTCGAGCAGC     Tm(°C): 59.826     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 320     End: 615     Product size (bp): 296
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Enzyme Id:  K03941

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103335651     NCBI Gene Symbol: LOC103335651
Gene Aliases
Gene description & Other designations Description:   NADH dehydrogenase [ubiquinone] iron-sulfur protein 8; mitochondrial      Other designations:   NADH dehydrogenase [ubiquinone] iron-sulfur protein 8; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG6     Strand:   plus     Exon count:   8
Gene Location within genomic sequence Genomic accession No. NC_024131.1      Gene Start and end within genomic accession: 11049657 ...... 11054181
CDS Sequence 103335651
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GAGGTCTGATGCTGACGCTT     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GTAGGGCATGTTCACCACGA     Tm(°C): 60.037     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 232     End: 339     Product size (bp): 108
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