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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     1
Number of PMTM primers:     2

Enzyme Id:  K03940

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103320537     NCBI Gene Symbol: LOC103320537
Gene Aliases
Gene description & Other designations Description:   NADH dehydrogenase [ubiquinone] iron-sulfur protein 7; mitochondrial      Other designations:   NADH dehydrogenase [ubiquinone] iron-sulfur protein 7; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 11272897 ...... 11274599
CDS Sequence 103320537
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CT)4ccacacgaccgtcccctcgctgtcg(TCC)3
Repeat start & end within CDS Repeat start: 58     Repeat end: 99
Forward primer Primer sequence:   ATCCCTCTCCAGCTCTCGG     Tm(°C): 60.153     GC (%): 63.158     Size: 19
Reverse primer Primer sequence:   GGCCCAGTTCATGAGATCGT     Tm(°C): 59.821     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 27     End: 224     Product size (bp): 198
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Enzyme Id:  K03940

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103320537     NCBI Gene Symbol: LOC103320537
Gene Aliases
Gene description & Other designations Description:   NADH dehydrogenase [ubiquinone] iron-sulfur protein 7; mitochondrial      Other designations:   NADH dehydrogenase [ubiquinone] iron-sulfur protein 7; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 11272897 ...... 11274599
CDS Sequence 103320537
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CTCCAG)2(AGA)3*
Repeat start & end within CDS Repeat start: 586     Repeat end: 604
Forward primer Primer sequence:   TCGGGGTTGTGACAGGATTG     Tm(°C): 59.964     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACTTCGTCCACCAAAGGAGG     Tm(°C): 59.603     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 503     End: 642     Product size (bp): 140
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Enzyme Id:  K03940

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103320537     NCBI Gene Symbol: LOC103320537
Gene Aliases
Gene description & Other designations Description:   NADH dehydrogenase [ubiquinone] iron-sulfur protein 7; mitochondrial      Other designations:   NADH dehydrogenase [ubiquinone] iron-sulfur protein 7; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   minus     Exon count:   2
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 11272897 ...... 11274599
CDS Sequence 103320537
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TGGGTCCATCTGGCCTATGA     Tm(°C): 60.03     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAATCCTGTCACAACCCCGA     Tm(°C): 59.964     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 230     End: 522     Product size (bp): 293
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