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Statistics

Number of enzymes: 1
Total Number of designed primers: 2
Number of PGTM primers:     2

Enzyme Id:  K03937

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103336521     NCBI Gene Symbol: LOC103336521
Gene Aliases
Gene description & Other designations Description:   NADH dehydrogenase [ubiquinone] iron-sulfur protein 4; mitochondrial      Other designations:   NADH dehydrogenase [ubiquinone] iron-sulfur protein 4; mitochondrial
Chromosome, Strand & Exon count Chromosome:   LG1     Strand:   minus     Exon count:   5
Gene Location within genomic sequence Genomic accession No. NC_024126.1      Gene Start and end within genomic accession: 19048137 ...... 19051112
CDS Sequence 103336521
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CATCTTCGCAGGAGGGTTGT     Tm(°C): 60.036     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCATACGGGTCACCAGTTGA     Tm(°C): 60.037     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 144     End: 295     Product size (bp): 152
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Enzyme Id:  K03937

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103320475     NCBI Gene Symbol: LOC103320475
Gene Aliases
Gene description & Other designations Description:   NADH dehydrogenase [ubiquinone] iron-sulfur protein 4; mitochondrial-like      Other designations:   NADH dehydrogenase [ubiquinone] iron-sulfur protein 4; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   5
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 10887131 ...... 10889087
CDS Sequence 103320475
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CGAACTGCAACCCAACAAGG     Tm(°C): 59.969     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CAGTCCTGCCTCACCAACAT     Tm(°C): 59.963     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 177     End: 314     Product size (bp): 138
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