image


Statistics

Number of enzymes: 1
Total Number of designed primers: 7
Number of PGTM primers:     2
Number of PMTM primers:     5

Enzyme Id:  K03527

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   111470927     NCBI Gene Symbol: LOC111470927
Gene Aliases
Gene description & Other designations Description:   4-hydroxy-3-methylbut-2-enyl diphosphate reductase; chloroplastic      Other designations:   4-hydroxy-3-methylbut-2-enyl diphosphate reductase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 111470927
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Compound     Repeat sequence: (CTTCTG)2(CTT)4
Repeat start & end within CDS Repeat start: 125     Repeat end: 148
Forward primer Primer sequence:   GAGAGTTTCCCCGGAGTTCG     Tm(°C): 60.109     GC (%): 60     Size: 20
Reverse primer Primer sequence:   CATAAGCGCCAGAGTCTCGT     Tm(°C): 59.899     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 57     End: 266     Product size (bp): 210
JBrowse View      JBrowse

Enzyme Id:  K03527

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   111470927     NCBI Gene Symbol: LOC111470927
Gene Aliases
Gene description & Other designations Description:   4-hydroxy-3-methylbut-2-enyl diphosphate reductase; chloroplastic      Other designations:   4-hydroxy-3-methylbut-2-enyl diphosphate reductase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 111470927
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (TG)4
Repeat start & end within CDS Repeat start: 801     Repeat end: 808
Forward primer Primer sequence:   AGAACATGGCAGAGGCAACA     Tm(°C): 59.889     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TTTTCGACTCCATGCTTGCG     Tm(°C): 59.483     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 778     End: 1018     Product size (bp): 241
JBrowse View      JBrowse

Enzyme Id:  K03527

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   111470927     NCBI Gene Symbol: LOC111470927
Gene Aliases
Gene description & Other designations Description:   4-hydroxy-3-methylbut-2-enyl diphosphate reductase; chloroplastic      Other designations:   4-hydroxy-3-methylbut-2-enyl diphosphate reductase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 111470927
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AACCA)2
Repeat start & end within CDS Repeat start: 934     Repeat end: 943
Forward primer Primer sequence:   AGAACATGGCAGAGGCAACA     Tm(°C): 59.889     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TTTTCGACTCCATGCTTGCG     Tm(°C): 59.483     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 778     End: 1018     Product size (bp): 241
JBrowse View      JBrowse

Enzyme Id:  K03527

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   111470927     NCBI Gene Symbol: LOC111470927
Gene Aliases
Gene description & Other designations Description:   4-hydroxy-3-methylbut-2-enyl diphosphate reductase; chloroplastic      Other designations:   4-hydroxy-3-methylbut-2-enyl diphosphate reductase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 111470927
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CAATA)2
Repeat start & end within CDS Repeat start: 1044     Repeat end: 1053
Forward primer Primer sequence:   CGCAAGCATGGAGTCGAAAA     Tm(°C): 59.483     GC (%): 50     Size: 20
Reverse primer Primer sequence:   TCCTGGGCCTATTCTCTGCT     Tm(°C): 60.031     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 999     End: 1229     Product size (bp): 231
JBrowse View      JBrowse

Enzyme Id:  K03527

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   111470927     NCBI Gene Symbol: LOC111470927
Gene Aliases
Gene description & Other designations Description:   4-hydroxy-3-methylbut-2-enyl diphosphate reductase; chloroplastic      Other designations:   4-hydroxy-3-methylbut-2-enyl diphosphate reductase; chloroplastic
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 111470927
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TTCTTCTTCGTCGTCGGTGG     Tm(°C): 60.04     GC (%): 55     Size: 20
Reverse primer Primer sequence:   ACCCTTGCCAACCACTTCAA     Tm(°C): 60.033     GC (%): 50     Size: 20
Primer start, end within sequence and product size Start: 140     End: 554     Product size (bp): 415
JBrowse View      JBrowse

Enzyme Id:  K03527

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   111484238     NCBI Gene Symbol: LOC111484238
Gene Aliases
Gene description & Other designations Description:   4-hydroxy-3-methylbut-2-enyl diphosphate reductase; chloroplastic-like      Other designations:   4-hydroxy-3-methylbut-2-enyl diphosphate reductase; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 111484238
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (CGG)3
Repeat start & end within CDS Repeat start: 69     Repeat end: 77
Forward primer Primer sequence:   ATTTCTCTGCAGATCCCCCG     Tm(°C): 59.528     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CTGTGCACCGAACCAAAAGG     Tm(°C): 59.969     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 6     End: 117     Product size (bp): 112
JBrowse View      JBrowse

Enzyme Id:  K03527

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   111484238     NCBI Gene Symbol: LOC111484238
Gene Aliases
Gene description & Other designations Description:   4-hydroxy-3-methylbut-2-enyl diphosphate reductase; chloroplastic-like      Other designations:   4-hydroxy-3-methylbut-2-enyl diphosphate reductase; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   Un     Strand:        Exon count:   
Gene Location within genomic sequence Genomic accession No.      Gene Start and end within genomic accession: ......
CDS Sequence 111484238
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   CGAGGAATCCCGTCGTACTG     Tm(°C): 59.971     GC (%): 60     Size: 20
Reverse primer Primer sequence:   GCCCATCAGGTAGCCAGTTT     Tm(°C): 60.034     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 1149     End: 1266     Product size (bp): 118
JBrowse View      JBrowse