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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     1
Number of PMTM primers:     2

Enzyme Id:  K03405

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108205276     NCBI Gene Symbol: LOC108205276
Gene Aliases DCAR_004025
Gene description & Other designations Description:   magnesium-chelatase subunit ChlI; chloroplastic-like      Other designations:   magnesium-chelatase subunit ChlI; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   1     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_030381.1      Gene Start and end within genomic accession: 44066539 ...... 44069715
CDS Sequence 108205276
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (ATGAA)2
Repeat start & end within CDS Repeat start: 278     Repeat end: 287
Forward primer Primer sequence:   AAATCAGCCCTGCCCAAAGT     Tm(°C): 60.179     GC (%): 50     Size: 20
Reverse primer Primer sequence:   ACGGGTCACCAACAACTACC     Tm(°C): 59.893     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 190     End: 426     Product size (bp): 237
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Enzyme Id:  K03405

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108205276     NCBI Gene Symbol: LOC108205276
Gene Aliases DCAR_004025
Gene description & Other designations Description:   magnesium-chelatase subunit ChlI; chloroplastic-like      Other designations:   magnesium-chelatase subunit ChlI; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   1     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_030381.1      Gene Start and end within genomic accession: 44066539 ...... 44069715
CDS Sequence 108205276
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Di     Repeat sequence: (GA)4
Repeat start & end within CDS Repeat start: 736     Repeat end: 743
Forward primer Primer sequence:   TTGGATTCTGCTGCCTCAGG     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   AGTTGCGGCCTAAGTTCTCC     Tm(°C): 60.036     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 702     End: 823     Product size (bp): 122
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Enzyme Id:  K03405

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   108205276     NCBI Gene Symbol: LOC108205276
Gene Aliases DCAR_004025
Gene description & Other designations Description:   magnesium-chelatase subunit ChlI; chloroplastic-like      Other designations:   magnesium-chelatase subunit ChlI; chloroplastic-like
Chromosome, Strand & Exon count Chromosome:   1     Strand:   plus     Exon count:   3
Gene Location within genomic sequence Genomic accession No. NC_030381.1      Gene Start and end within genomic accession: 44066539 ...... 44069715
CDS Sequence 108205276
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   TTGGATTCTGCTGCCTCAGG     Tm(°C): 60.035     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CCTTTGCTGCCCTGTTTGTC     Tm(°C): 59.967     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 702     End: 1107     Product size (bp): 406
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