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Statistics

Number of enzymes: 1
Total Number of designed primers: 3
Number of PGTM primers:     1
Number of PMTM primers:     2

Enzyme Id:  K03111

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   PHAVU_003G178400g     NCBI Gene Symbol: PHAVU_003G178400g
Gene Aliases PHAVU_003G178400g
Gene description & Other designations Description:   hypothetical protein      Other designations:   hypothetical protein
Chromosome, Strand & Exon count Chromosome:   3     Strand:   minus     Exon count:   4
Gene Location within genomic sequence Genomic accession No. NC_023757.1      Gene Start and end within genomic accession: 39035844 ...... 39038362
CDS Sequence PHAVU_003G178400g
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (CACCCG)2
Repeat start & end within CDS Repeat start: 164     Repeat end: 175
Forward primer Primer sequence:   GCGTATCACCACCTTCCACA     Tm(°C): 60.037     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GCCTCCGATTGTTGCGAATC     Tm(°C): 59.972     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 63     End: 360     Product size (bp): 298
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Enzyme Id:  K03111

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   PHAVU_003G178400g     NCBI Gene Symbol: PHAVU_003G178400g
Gene Aliases PHAVU_003G178400g
Gene description & Other designations Description:   hypothetical protein      Other designations:   hypothetical protein
Chromosome, Strand & Exon count Chromosome:   3     Strand:   minus     Exon count:   4
Gene Location within genomic sequence Genomic accession No. NC_023757.1      Gene Start and end within genomic accession: 39035844 ...... 39038362
CDS Sequence PHAVU_003G178400g
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (CGAGAA)2
Repeat start & end within CDS Repeat start: 366     Repeat end: 377
Forward primer Primer sequence:   GATTCGCAACAATCGGAGGC     Tm(°C): 59.972     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CTTTGGTCTCGAGGTTCCCC     Tm(°C): 60.036     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 341     End: 513     Product size (bp): 173
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Enzyme Id:  K03111

Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   PHAVU_003G178400g     NCBI Gene Symbol: PHAVU_003G178400g
Gene Aliases PHAVU_003G178400g
Gene description & Other designations Description:   hypothetical protein      Other designations:   hypothetical protein
Chromosome, Strand & Exon count Chromosome:   3     Strand:   minus     Exon count:   4
Gene Location within genomic sequence Genomic accession No. NC_023757.1      Gene Start and end within genomic accession: 39035844 ...... 39038362
CDS Sequence PHAVU_003G178400g
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   GATTCGCAACAATCGGAGGC     Tm(°C): 59.972     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CTTTGGTCTCGAGGTTCCCC     Tm(°C): 60.036     GC (%): 60     Size: 20
Primer start, end within sequence and product size Start: 341     End: 513     Product size (bp): 173
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