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Statistics

Number of enzymes: 1
Total Number of designed primers: 6
Number of PGTM primers:     2
Number of PMTM primers:     4
Number of Failed designed PMTM primers: 1

Enzyme Id:  K02265
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103328034     NCBI Gene Symbol: LOC103328034
Gene Aliases
Gene description & Other designations Description:   putative cytochrome c oxidase subunit 5b-like      Other designations:   putative cytochrome c oxidase subunit 5b-like
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 5580619 ...... 5584418
CDS Sequence 103328034
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (AGGGA)2
Repeat start & end within CDS Repeat start: 242     Repeat end: 251
Forward primer Primer sequence:   AGCTCAAGGCCCTAACCCTA     Tm(°C): 59.956     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CATCATGCTCATCCTCGCCT     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 28     End: 375     Product size (bp): 348
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Enzyme Id:  K02265
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103328034     NCBI Gene Symbol: LOC103328034
Gene Aliases
Gene description & Other designations Description:   putative cytochrome c oxidase subunit 5b-like      Other designations:   putative cytochrome c oxidase subunit 5b-like
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 5580619 ...... 5584418
CDS Sequence 103328034
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Hexa     Repeat sequence: (ATGACC)3
Repeat start & end within CDS Repeat start: 482     Repeat end: 499
Forward primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Reverse primer Primer sequence:   None     Tm(°C):      GC (%):      Size:
Primer start, end within sequence and product size Start:      End:      Product size (bp):
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Enzyme Id:  K02265
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103328034     NCBI Gene Symbol: LOC103328034
Gene Aliases
Gene description & Other designations Description:   putative cytochrome c oxidase subunit 5b-like      Other designations:   putative cytochrome c oxidase subunit 5b-like
Chromosome, Strand & Exon count Chromosome:   LG4     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024129.1      Gene Start and end within genomic accession: 5580619 ...... 5584418
CDS Sequence 103328034
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   AGCTCAAGGCCCTAACCCTA     Tm(°C): 59.956     GC (%): 55     Size: 20
Reverse primer Primer sequence:   CATCATGCTCATCCTCGCCT     Tm(°C): 59.965     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 28     End: 375     Product size (bp): 348
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Enzyme Id:  K02265
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103320065     NCBI Gene Symbol: LOC103320065
Gene Aliases
Gene description & Other designations Description:   cytochrome c oxidase subunit 5b-2; mitochondrial-like      Other designations:   cytochrome c oxidase subunit 5b-2; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 8767630 ...... 8770041
CDS Sequence 103320065
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (CTCAG)2
Repeat start & end within CDS Repeat start: 27     Repeat end: 36
Forward primer Primer sequence:   AGGCGAGTGGCATCTTCTTC     Tm(°C): 60.108     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TATCGCTGGTGCTTCCTTGG     Tm(°C): 60.108     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 6     End: 329     Product size (bp): 324
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Enzyme Id:  K02265
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103320065     NCBI Gene Symbol: LOC103320065
Gene Aliases
Gene description & Other designations Description:   cytochrome c oxidase subunit 5b-2; mitochondrial-like      Other designations:   cytochrome c oxidase subunit 5b-2; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 8767630 ...... 8770041
CDS Sequence 103320065
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Tri     Repeat sequence: (GAA)3
Repeat start & end within CDS Repeat start: 192     Repeat end: 200
Forward primer Primer sequence:   ATCTCACCCGTCGGTTCCTA     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TATCGCTGGTGCTTCCTTGG     Tm(°C): 60.108     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 79     End: 329     Product size (bp): 251
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Enzyme Id:  K02265
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103320065     NCBI Gene Symbol: LOC103320065
Gene Aliases
Gene description & Other designations Description:   cytochrome c oxidase subunit 5b-2; mitochondrial-like      Other designations:   cytochrome c oxidase subunit 5b-2; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 8767630 ...... 8770041
CDS Sequence 103320065
Marker Information
Marker Type PMTM
Repeat type & sequence Repeat type: Penta     Repeat sequence: (GAAAG)2
Repeat start & end within CDS Repeat start: 420     Repeat end: 429
Forward primer Primer sequence:   GCGCTTCCTCTTCTGCTTTG     Tm(°C): 59.832     GC (%): 55     Size: 20
Reverse primer Primer sequence:   GGGCCAACCACTTCCAACTT     Tm(°C): 60.759     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 172     End: 481     Product size (bp): 310
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Enzyme Id:  K02265
Gene Information
NCBI Gene Accession Number & Symbol Accession Number:   103320065     NCBI Gene Symbol: LOC103320065
Gene Aliases
Gene description & Other designations Description:   cytochrome c oxidase subunit 5b-2; mitochondrial-like      Other designations:   cytochrome c oxidase subunit 5b-2; mitochondrial-like
Chromosome, Strand & Exon count Chromosome:   LG2     Strand:   plus     Exon count:   6
Gene Location within genomic sequence Genomic accession No. NC_024127.1      Gene Start and end within genomic accession: 8767630 ...... 8770041
CDS Sequence 103320065
Marker Information
Marker Type PGTM
Repeat type & sequence Repeat type:      Repeat sequence:
Repeat start & end within CDS Repeat start:      Repeat end:
Forward primer Primer sequence:   ATCTCACCCGTCGGTTCCTA     Tm(°C): 60.034     GC (%): 55     Size: 20
Reverse primer Primer sequence:   TATCGCTGGTGCTTCCTTGG     Tm(°C): 60.108     GC (%): 55     Size: 20
Primer start, end within sequence and product size Start: 79     End: 329     Product size (bp): 251
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